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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttattagtttcttggaaatgcttacttctattagtttctgatcttataagtaatcaaatgattaccttaccccaaggattaaacatttacataaattttattagttatctgctggatgtggtccttccaccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG

Basic information

species Zea mays
transcript GRMZM2G047894_T02
intron # 12
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G047894_T01 (Zea mays), 3'ss of exon 12
lower sequence: LOC_Os03g18080.2 (Oryza sativa), 3'ss of exon 12
--gttattagtttcttggaaat------gcttacttctattagtttctgatcttataagtaatcaaatgattaccttaccccaa-ggattaaacatttacataaattttattagttatctgctggatgtggtccttccaccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG
  | |    ||||| || ||||         | ||||||||  ||| ||||  || |||| || ||||   |  ||      ||  |  | | ||| || ||||| || || | || | |||||    || ||  |  | || |||||||| | |||||||||||||||||| | ||||||||||| |||||||| ||| |||||| ||| |||||||||||||| ||||||||||| || |||||
gtgcttgatgtttcgtgaaaatcataagatatccttctattgatttgtgattataaaagtgataaaatatctttctgggattaacaggattagcatgtatataaaatt-atcattt-tatgctga---tgttcactttatct-acagGTATGTGCATTTTGATTTCCATCATATCTGTCGTGGAGGGAACTTTGATAATCTGCAAGTTCTATACAATCAAATTGAAGAAGCTATTCAGAAACAAGG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|31348859|gb|CD433216.1|CD433216
EST:     GATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAG                         GTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
genomic: GATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
EST: gi|40335862|gb|CK369932.1|CK369932
EST:     GATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAG                         GTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
genomic: GATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
EST: gi|71311517|gb|DR792122.1|DR792122
EST:     GATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAG                         GTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
genomic: GATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
EST: gi|60343346|gb|DN210319.1|DN210319
EST:     ATGGACAGAATTCCTGGTGTCAG                         GTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA
genomic: ATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 97

CGAGGTGATGAAGGAGATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 124

CGAGGTGATGAAGGAGATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 156

CGAGGTGATGAAGGAGATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 169

CGAGGTGATGAAGGAGATTTGAGCAATGCATTTGCTGCTGAAATGGACAGAATTCCTGGTGTCAGgttattagtt ... caccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

acatttacataaattttattagttatctgctggatgtggtccttccaccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG
                                       tccttccaccttac  CT-rich tract
     tacataaattttatta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttattagtttcttggaaatgcttacttctattagtttctgatcttataagtaatcaaatgattaccttaccccaaggattaaacatttacataaattttattagttatctgctggatgtggtccttccaccttacagGTATATACATTTTGATTTCCATCATGTTTGTCGTGGAGGTAACTTTGACAATTTGCAAGCTCTCTACAATCAAATTGAGGAAGCTATTCATAAGCAAGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCAAG