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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttcgtgcaatattacttttgttgtgctgtcttaaattgcttctaactttctggttctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG

Basic information

species Zea mays
transcript GRMZM2G416120_T01
intron # 11
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: LOC_Os10g32550.1 (Oryza sativa), 3'ss of exon 12
gttcgtgcaatattacttttgttgtgctgtct-taaattgcttctaactttctggtt-ctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
|||||| ||| |||| ||   ||| | |     |  |||  ||  ||  |  |||||  |||||  ||||     ||  ||  ||  |||||||||||||||| ||| |||||   |||||||| |||||||||||||||||||| ||||| ||||||||||||||||| ||||||||||||||||| |||
gttcgtacaacattaattc-attgcgttactagtgcattattttgaaaatcgtggttgttgatagattat-----ataaatgctg--tgttgcagATTAGATCTGCAATTGAGCTAAGCACTTCTGATTATGACAAGGAAAAGCTACAGGAGCGGTTGGCAAAGCTCTCCGGAGGTGTTGCTGTTCTAAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA20G33910.1 (Glycine max), 3'ss of exon 12
---------------------gttcgtgcaatattacttttgttgtg--ctgtcttaaattgcttctaactttctggttctgataatttatggattatg--gattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
                      ||  |    ||  | | ||| |||   ||||||| |  | ||  || |||  || |  |  | | || |   || ||  || | |  |     ||||||||||||||| |||| || ||||||||||||||||||||||||||| | |||||| | || || ||||| ||||| |||||||| || ||||||
gtctgtttgaatcttacattattttttattctaccattgttggtgtattctgtcttcatgtactgata-cttattgatgtttttgacttgtttcttgtgatgaattttaacaa-acagATTAGATCAGCAATTGAAAATAGCACTTCAGATTATGACAAGGAAAAGTTACAGGAAAGATTAGCCAAGCTTTCTGGGGGTGTTGCAGTACTGAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA20G19980.1 (Glycine max), 3'ss of exon 12
gttcgtgc--aatattacttttgttgtgctgtcttaaattgct-tctaactttctg---gttctgataattta--tggattatggattatggatgttg-----cagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
||| | |   | |  ||| |||| |    ||| ||  ||| |  || ||   ||||   | | | || ||  |  |   || |   | ||| || |||     |||||||| |||||| |||| ||||| || ||||||||||||||||||||| | |||||| | || || ||||| ||||| |||||||| || || |||
gtttgagttgatttatacatttggtcccgtgtattctatttccatccaatgatctgtaaggtttaatgatgcacctaacttgtttgtgatgaattttgataaacagATTAGGTCAGCAATTGAAAACAGTACCTCAGATTATGACAAGGAAAAGTTACAGGAAAGATTAGCCAAGCTTTCTGGTGGTGTTGCAGTGCTTAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA10G25630.1 (Glycine max), 3'ss of exon 12
------gttcgt--gcaatattacttttgttgtgctgtcttaaattgcttcta--actttctggttctgataattta--tggattatggattatggatgttg-----cagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
      |||  |    |   ||  ||||    || | |  ||  |     |||  |  |    | | | || ||  |  ||  || |   | ||| || |||     |||||||| || ||| |||| |||||||| ||||||||||||||||||||| | |||||| | || || ||||| ||||| |||||||| || || |||
gtttgagttgatttatagatttggttttcccatggtattctatttccaccctaggatctgtaaggtttaatgatgcacctgacttgtttgtgatgaattttgataaacagATTAGGTCTGCAATTGAAAACAGCACCTCAGATTATGACAAGGAAAAGTTACAGGAAAGATTAGCCAAGCTTTCTGGTGGTGTTGCCGTGCTTAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA10G33680.1 (Glycine max), 3'ss of exon 12
---------------------------gttcgtgcaatattacttttgttgtgctgtcttaaattgcttctaactttctggttctgataatttatggattatg--gattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
                            ||  |    ||  | | ||| |||  | | ||  ||    |   ||||  || |  |  | | || |   || ||  || | |  |    |||||||||||||||| |||| || ||||||||||||||||||||||||||| | |||||| | || || ||||| ||||| |||||||| || ||||||
gtctgtttgaatcttccactattattaatttttattctaccattgttggtgtattctgtttcatgtactgatacttattgatgttttttacttgtttcttgtgatgaattttaacaa-gcagATTAGATCAGCAATTGAAAATAGCACTTCAGATTATGACAAGGAAAAGTTACAGGAAAGATTAGCCAAGCTTTCTGGGGGTGTTGCAGTACTGAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: AT2G33210.1 (Arabidopsis thaliana), 3'ss of exon 11
---------gttcgtgcaatattac--ttttgttgtgctgtcttaaattgcttctaactttctggtt-ctgataattt--atggattatggattat----ggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
          ||   ||  | |||   ||| || || | || | | ||  |||    |||| |     ||| || |||  ||   || || ||| |      |    ||||||  ||||    |||||    |||||||| |||||||||||||| ||| | || ||| ||||||| ||||| || || ||||||||||| || |||
gttagcgtgtttttcgcttttttatcatttagtggttcagtgtcatatacctttgcccttttttccaactggtagttttaatatgttttgaatttttattaaaccacgcagATACGATCCATGGTTGAAGCAAGCACTTCTGATTATGACAAGGAGAAGTTGCAAGAAAGGTTGGCTAAGCTTTCGGGTGGTGTTGCTGTACTAAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: AT3G23990.1 (Arabidopsis thaliana), 3'ss of exon 10
-----------gttcgtgcaatattacttttgtt-gtg-------ctgtcttaaattgcttct-aactttctggttctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
             |  | |  |  ||  || ||| ||         ||| |  ||    || | || | | ||    |  |   || |   |  | | |  || |  |  |||||||| |||||| ||||    ||||| |||||||||||||||||||| || || ||| ||||||| |||||||||||||||||||| ||  |||||
gtaatttttaaccttataccctgataaattggttagtaataaaatttgtatgcaagaaattatgaagtgtatgaaagtagttcgttctaattgcttggtattgcaactaacagATTAGGTCAGCAATTGAACTGAGCACCTCAGATTATGACAAGGAAAAACTGCAAGAAAGGTTGGCTAAGCTCTCTGGAGGTGTTGCAGTGTTGAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: Vv05s0051g00340.t01 (Vitis vinifera), 3'ss of exon 12
--------------------------------------------------------------------------------------gttcgtgcaatattacttttgttgtgctg----tcttaaattgcttctaactttctggttctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
                                                                                        |||     | || | ||  ||| |  |    |   |||||   | ||  ||| |  |  ||  | |  ||  |||  |    || |   | ||||||||| |||||  ||||    |||||||| |||||||||||||||||| | || ||| ||||||| ||||| ||||| |||||||||||  |||||
gtccatattactttcttctcacatgttatttagaatctcctgattctttattctttttctttcttttcttctctggatgattgatctgtcgattggtttttccttcattgcgaagcaaatgccaaattttatatatttttttcatgttgtaattggattcatttggttacattgggatggcagATTAGGTCAGCCATTGAATTGAGCACTTCTGATTATGACAAGGAAAAGTTGCAAGAAAGGTTGGCCAAGCTTTCTGGTGGTGTTGCTGTCTTGAAG

upper sequence: GRMZM2G416120_T01 (Zea mays), 3'ss of exon 11
lower sequence: EFJ32351 (Selaginella moellendorffii), 3'ss of exon 10
gttcgtgcaatattacttttgttgtgctgtcttaaattgcttctaactttct-ggttctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
                                           ||||   ||  | || ||      | |   ||         | | |||||||| |||  ||| ||  ||   ||||| ||||| |||||||||||||||||||| ||| || |||| ||||||||||||||||| || ||  |||||
------------------------------------------gtaacgcacttagctcagaggg---aagagatacctgacgccgttattgcagATCAGAGAAGCTGTGAAGTCTAGCACATCAGACTATGACAAGGAAAAGCTCCAAGAAAGGCTGGCCAAGCTCTCTGGAGGTGTAGCCGTCTTGAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|50328354|gb|CO523480.1|CO523480
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAA
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAA
EST: gi|31349659|gb|CD434016.1|CD434016
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|91875568|gb|EB405525.1|EB405525
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|71425566|gb|DR806758.1|DR806758
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|78078493|gb|DV506906.1|DV506906
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|32929020|gb|CF033832.1|CF033832
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACGGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|18450761|gb|BM429039.1|BM429039
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|87152342|gb|DY397131.1|DY397131
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|32944170|gb|CF048989.1|CF048989
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|32932633|gb|CF037445.1|CF037445
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|32931719|gb|CF036531.1|CF036531
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|6056565|gb|AW090970.1|AW090970
EST:     TTCTTGATGGCGCTGGAGACCAAAAGGCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAATTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|21432198|gb|BQ547688.1|BQ547688
EST:     TCTTGATGGCGCTGGTAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TCTTGATGGCGCTGG-AGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|32857688|gb|CD997369.1|CD997369
EST:     TCTTGATGGCGCTGGAGACAAAAAGTGCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGGTGAGAACTGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TCTTGATGGCGCTGGAGACAAAAAGT-CTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|33103385|gb|CF063345.1|CF063345
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
EST: gi|22489874|gb|BU049797.1|BU049797
EST:     TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAG                         ATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC
genomic: TTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 481

GTGACTGTCTCTAAGGATGACACTGTTATTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 212

GTGACTGTCTCTAAGGATGACACTGTTATTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 168

GTGACTGTCTCTAAGGATGACACTGTTATTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 307

GTGACTGTCTCTAAGGATGACACTGTTATTCTTGATGGCGCTGGAGACAAAAAGTCTATTGAAGAGAGGGCAGACCAGgttcgtgcaa ... gatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gcttctaactttctggttctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG
                     ataatttatggattat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttcgtgcaatattacttttgttgtgctgtcttaaattgcttctaactttctggttctgataatttatggattatggattatggatgttgcagATTAGATCAGCAGTTGAGAACAGCACTTCAGATTATGACAAGGAAAAGCTCCAGGAACGGTTGGCAAAGCTCTCTGGAGGTGTTGCTGTTCTGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG