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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttagattgtcctctcgttgtcttatgcattccattatttggtacctctgcttatggttttggagtttactttatacctacgttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG

Basic information

species Zea mays
transcript GRMZM2G164743_T01
intron # 10
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G164743_T01 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os04g44430.1 (Oryza sativa), 3'ss of exon 8
gttagattgtcctctcgtt-------gtcttatgcattccattat--ttggtac-ctctgct-tatggttttggagtttactttatacctacgttatttca--gGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG
||| || | || |   | |       ||| |||  |    |||||   |||||   ||| || | |||   ||||||| | ||    |||  | | || ||  ||||| ||||| |||||||| ||||||||||| ||||||||||||||||| ||||| ||||| || ||||||||||||
gtttgagt-tcatactgctaaaaagagtcatatttaaattattatatctggtagattctactatttgggactggagttcatttc---cctgtgctgttccacagGTTACATCTCTTACAACTTTGTCATTGGTGCCTATTCCTACTGGGGCCCTAAGGCTGGTCAGGATATCTACAATATG

upper sequence: GRMZM2G164743_T01 (Zea mays), 3'ss of exon 10
lower sequence: GLYMA09G33680.1 (Glycine max), 3'ss of exon 7
---gttagattgtcctctcgttgtcttatgcattccatt---atttggtacctctgcttatggttttggagt--ttactttatacctacgttatt-tcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG
    | |  ||    | ||    | | ||   |   |||   ||||   |   |    |||||| || |  |  |||| | | |  |     |||  |||| || ||  | ||||| || |||||||| ||||| || ||||||||||||||||||||  |    || ||| |||||
tgaatgaatttcagttttcagaatttgatatttcatatttgtattttcaattccgaactatggtatttgtttctttacataagaatttaaacattgccagGGTACATAGCATACAATTTTGTCATTGGAGCTTACTCATACTGGGGCCCAAAGGCAGGATACAGCATTTACCATATG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|5325067|gb|AI783258.1|AI783258
EST:     AGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAG                         GTTATATTTTCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAA
genomic: AGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAA
EST: gi|6056590|gb|AW090995.1|AW090995
EST:     AGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGNTGTCTTAG                         GTTATATTTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAA
genomic: AGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAA
EST: gi|5846879|gb|AI999962.1|AI999962
EST:     AGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAG                         GTTATATTTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAA
genomic: AGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 108

GTCCAGTTTTTGCCATGTTCTCATGACTGAAATTGGTCGCTTCAGCAAAGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 132

GTCCAGTTTTTGCCATGTTCTCATGACTGAAATTGGTCGCTTCAGCAAAGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 123

GTCCAGTTTTTGCCATGTTCTCATGACTGAAATTGGTCGCTTCAGCAAAGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 91

GTCCAGTTTTTGCCATGTTCTCATGACTGAAATTGGTCGCTTCAGCAAAGATATGAAACAACTATTACAAGAGAAGGTTTTTGTCATTGTTGTCTTAGgttagattgt ... gttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atttggtacctctgcttatggttttggagtttactttatacctacgttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG
                                              ttatttc  CT-rich tract
                             tttactttata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttagattgtcctctcgttgtcttatgcattccattatttggtacctctgcttatggttttggagtttactttatacctacgttatttcagGTTATATCTCCTACAACTTCGTCATTGGTGCTTATTCCTACTGGGGCCCAAAGGCAGGTCAAGAGATCTACAATATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATC