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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaattcagtggtttgattggggtgcgatccgttgggtttttattcacatgttggtgttgttcttcactaacgcaacatttggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC

Basic information

species Zea mays
transcript GRMZM2G144372_T01
intron # 10
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G144372_T01 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os05g51530.2 (Oryza sativa), 3'ss of exon 10
gtaattcagtggtttgattggggtgcgatccgttgggtttttattcacatgttggtgttgttcttcactaacgcaacatttggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC
||||||||| | ||   || | || |  | | |||  ||  |    |||||    || | ||  ||| |||   | | | || |  ||||  | || |||||||| ||||||||||| || || |||||||||||||| ||||||||||| ||||| || || |||||||| ||||||||| ||||||  
gtaattcagcgtttgagtttgtgttcattgcattg--ttagtcacaacatgcacctgct-tttgtcattaattgagcctctgaattgcagTATTTACTGGAAATCTGAAGACGATGTTGGTGTAGCTGGTCTGGGAGGTGAAACCGAGGCTCACCCCTATGTTTCCTTCACAATAAATTTTATCTGAGCA

upper sequence: GRMZM2G144372_T01 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os05g51530.1 (Oryza sativa), 3'ss of exon 11
gtaattcagtggtttgattggggtgcgatccgttgggtttttattcacatgttggtgttgttcttcactaacgcaacatttggaacgcagCCTGTA-TTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCAC----------CATAAATTTTGTCTGAGAC
           ||   |  | ||  |   ||  ||   |||  ||||||  |   | || |        | | ||  || |     ||  |||||     |  |    |      || ||   || ||  | ||| |  | |||     ||| |   ||  || |   | |           | | |||| | |||     
-----------gtgaaaccgaggctcacccctatg---tttccttcaca-ataaattttatctg-----agcacagtatgttccttgctacctgtggccagGCAAGGCAGTGTTTTGGAGTAATTACTACTTTGGCAAACAAAAAACCAGCGAATGGTTCGGTGTATATTATAATTGTGATACGTCAATTCTTTCTTGATT

upper sequence: GRMZM2G144372_T01 (Zea mays), 3'ss of exon 10
lower sequence: Vv17s0000g00460.t01 (Vitis vinifera), 3'ss of exon 10
------------gtaattcagtggtttgattggggtgcgatccgttgggtttttattcacatgttggtgttgttcttcactaacgcaacatttggaacgcagCCTGTATTGGAAATCCGAAGACGA---TGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC
            ||            ||| |    ||   |  ||    |||||     ||   ||||  || |  |   |    ||    | | |  | |||   ||||||||||||||||| ||   || |||  | |   | || ||||| ||  | ||  | || |||||||||||||||||||||||| | ||||||   
atctgttttgttgtcgcaggaccacctgagtcttctgacttaggtcaaatttttgactgcaa--tggttctgattctatttgttacatgcatggaattgtagCACATATTGGAAATCCGAAGATGAACTTGGAGGTATGGGCAGCCTAGGAGGTGATGCTGATTCTCATCCTTACGTCTCCTTCACCATAAATCTGGTCTGA---

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60396077|gb|DN228946.1|DN228946
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|211035708|gb|FL440354.1|FL440354
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|32790927|gb|CD943163.1|CD943163
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|16377910|gb|BI992182.1|BI992182
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGGCGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|116823457|gb|EC867003.1|EC867003
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGATCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|60394318|gb|DN227188.1|DN227188
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|37387887|gb|CF631146.1|CF631146
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|40336015|gb|CK370085.1|CK370085
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGA
EST: gi|89248670|gb|DY620456.1|DY620456
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|76012957|gb|DT940127.1|DT940127
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|71428527|gb|DR809577.1|DR809577
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|211396248|gb|FL321770.1|FL321770
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|40335990|gb|CK370060.1|CK370060
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|61117885|gb|DN558846.1|DN558846
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|31664147|gb|CD573327.1|CD573327
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|61117883|gb|DN558844.1|DN558844
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|37397212|gb|CF635901.1|CF635901
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCTTAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|211430939|gb|FL367561.1|FL367561
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|5058953|gb|AI737429.1|AI737429
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGATCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|32910803|gb|CF015615.1|CF015615
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGATCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|71430058|gb|DR811108.1|DR811108
EST:     GAGAAGAAAGTAAGGAGCATTTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTTTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|37395039|gb|CF634799.1|CF634799
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|116819164|gb|EC862261.1|EC862261
EST:     TTAGACGAGCTCTCTGGGCGATG-CCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAAGCGTTGCTGGTCTGGGAGGCGA
genomic: TTAGACGAGCTCTCT-GGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|67010828|gb|CO439577.1|CO439577
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTANGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|31358589|gb|CD442946.1|CD442946
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|20811126|gb|BQ295604.1|BQ295604
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGATCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|40336709|gb|CK370779.1|CK370779
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|67022208|gb|CO450957.1|CO450957
EST:     GAGAGGAAAGTA-GGAGCATCTTATACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGANAG-CG
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGG-AGGCG
EST: gi|37390080|gb|CF632266.1|CF632266
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|60342510|gb|DN209483.1|DN209483
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
EST: gi|61117882|gb|DN558843.1|DN558843
EST:     GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAG                         CCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA
genomic: GAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 320

TCTGCTGTTCTTGCACCATCTACAAAGAGCGAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 319

TCTGCTGTTCTTGCACCATCTACAAAGAGCGAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 191

TCTGCTGTTCTTGCACCATCTACAAAGAGCGAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 395

TCTGCTGTTCTTGCACCATCTACAAAGAGCGAGAAGAAAGTAAGGAGCATCTTAGACGAGCTCTCTGGCGATGCCCATAGgtaattcagt ... tggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ggtttttattcacatgttggtgttgttcttcactaacgcaacatttggaacgcagCCTGTATTGGAAATCCGAAGACGATGTAGGCGTTGCTGGTCTGGGAGGCGAAACCGAGGCGCACCCTTACGTCTCCTTCACCATAAATTTTGTCTGAGAC
                              cactaac  putative branch site (score: 395)
     ttattca  TA-rich tract