ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...acataatcatgtttctgtcagcctcatgtaatgtcaatctgtaattttccccaaacaacttgtaatagtaatatcatgtttatgtgtgtctgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAATGATCAAAAAGGGATAATACCTCGTTCTTTGG

Basic information

species	Zea mays
transcript	GRMZM2G114861_T01
intron #	10
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G114861_T01 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os04g53760.2 (Oryza sativa), 3'ss of exon 11

acataatcatgtttctgtcagcctcatgtaatgtcaatctgtaatttt--ccccaaacaacttgtaatagtaatatcatgtttatgtgtgtctgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAATGATCAAAAAGGGATAATACCTCGTTCTTTGG
  || | |||  |  ||  |  || |  | ||||   |   | ||||        ||  |||||| |   |  |||  |     | | | |    ||| ||||| ||||||||||| ||||||||||| || |||||||||||||||||||||||||||  ||||||   |||||| ||||| || ||||||||||||||||
-aatcaccattatcttgaaactctgac-tgatgtttctagtttatttcagttttgaagtacttgtcaatttggtatgcttaagttatttttgcatgatgcagGTTTGCATATTTGCTTATGGTCAAACAGGGTCTGGTAAAACTTATACAATGATGGGTCCACCAGGGCGTGATCAGAAAGGCATCATACCTCGTTCTTTGG

upper sequence: GRMZM2G114861_T01 (Zea mays), 3'ss of exon 10
lower sequence: AT4G27180.1 (Arabidopsis thaliana), 3'ss of exon 11

----------------------------------------------acataatcatgtttctgtcagcctca---tgtaat---gtcaatctgt--aattttccccaaacaacttgtaat-----agtaatatcatgtttatgtgtgtctgc---ga----------------------tacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAAT---GATCAAAAAGGGATAATACCTCGTTCTTTGG
                                               ||| ||    || ||   |   |||   |||| |   || || ||||  || |   |   || | |   | |      | |||| | ||||||   |||  || |   ||                      | ||||| ||||| || |||||||| ||||| |||||||| ||||||||||||||||||||||  ||||||||    ||| |||||||  | || ||||| | ||   
gtactatctgctttagcttagtttaactccctgtttacttatgtcttcattattcgattcctcaaattttcagagtgtattttggtaaaactgtctaactggtcaataaaagcacatgaatgatgaataatgttatgtttcactgtaccttcttggactaaaggttctattacacaattttcagGTTTGCATTTTCGCATATGGACAAACAGGATCTGGAAAAACTTATACAATGATGGGTAGGCCAGGAAACCCGGATGAAAAAGGACTGATTCCTCGATGTT---

upper sequence: GRMZM2G114861_T01 (Zea mays), 3'ss of exon 10
lower sequence: AT5G54670.1 (Arabidopsis thaliana), 3'ss of exon 11

acataatcatgtttctgtcagcctcatgtaatgtcaatctgtaattttccccaaacaacttgtaatagtaatatcatgtttatgtgtgtctgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAATG---ATCAAAAAGGGATAATACCTCGTTCTTTGG
   |||  |  |  |||  |    ||   | |   |   |||   ||||     ||   ||||   | |||    | | | ||  |  ||     || |||| ||||| ||||||||||| ||||||||||| || |||||||| || ||||||||||  |||||||| |   |  |||||||  | || || || | ||   
--gtaactaaat--ctgcaaatggcaaaaagtacta---tgtgcgtttc-----actgtttgttggaataac--taagctgata-gaatccattttatagGTGTGCATTTTTGCATATGGACAAACTGGATCGGGAAAAACTTACACCATGATGGGTAGGCCAGGAAACGTCGAGGAAAAAGGTCTGATCCCGCGATGTT---

upper sequence: GRMZM2G114861_T01 (Zea mays), 3'ss of exon 10
lower sequence: Vv00s0532g00020.t01 (Vitis vinifera), 3'ss of exon 11

acataatcat-gtttctgtcagcctcatgtaatgtcaat-ctgtaattttcccc-aaacaacttgtaatagtaatatcatgtttatgtgt-gtctgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAAT---GATCAAAAAGGGATAATACCTCGTTCTTTGG
      | || | ||      | || ||    | | |||  |||||  | |    ||| |  |||||  | |   ||    ||  ||| | ||   |  |  |||| |||||||||||||||||||| || || ||||||||||| |||||||||||||| |  |||| |  |   ||| | |||||  | |||||||| || |   
----gcttattggttgcaattgtctgattacctcttaatgttgtaaaatgcaatgaaatattttgtataattctcatttgattcttgttttgttctctttgtagGTTTGCATATTTGCATATGGTCAGACAGGTTCTGGTAAAACCTATACAATGATGGGAAGGCCAGAAGCTTCTGATGAGAAAGGCCTGATACCTCGGTCAT---

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|22520949|gb|BU079760.1|BU079760

EST:     TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAG                         GTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG
genomic: TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG

EST: gi|67019346|gb|CO448095.1|CO448095

EST:     TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAG                         GTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG
genomic: TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG

EST: gi|87155658|gb|DY400447.1|DY400447

EST:     TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAG                         GTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG
genomic: TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG

EST: gi|67014413|gb|CO443162.1|CO443162

EST:     TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAG                         GTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG
genomic: TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG

EST: gi|78085386|gb|DV513779.1|DV513779

EST:     GGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAG                         GTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG
genomic: GGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG

EST: gi|26559172|gb|CA831407.1|CA831407

EST:     TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAG                         GTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG
genomic: TGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 238

CTCCTTCTCATATGACAAGGTTTTTGACCATGGTGCGTCACAGGAAGATGTGTTTGTGGAAATATCGCAACTAGTCCAAAGTGCCCTTGATGGGTACAAGgtactaactt ... tgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAATGATCAAAAAGGGATAATACCTCGTTCTTTGG

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 61

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 63

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 394

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tttccccaaacaacttgtaatagtaatatcatgtttatgtgtgtctgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAATGATCAAAAAGGGATAATACCTCGTTCTTTGG

                 taatagtaatatcatg  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
acataatcatgtttctgtcagcctcatgtaatgtcaatctgtaattttccccaaacaacttgtaatagtaatatcatgtttatgtgtgtctgcgatacagGTATGCATATTTGCATATGGTCAAACTGGATCTGGTAAAACTTATACAATGATGGGTAAACCAGGAAATGATCAAAAAGGGATAATACCTCGTTCTTTGG

- - - -catgttt
- - - - -tgtttct
- - - - - - - - - - - - catgtaa
- - - - - - - - - - - - - - - - - - - - - - - - - - -aacaact