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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagcatttagttctacatgtcacttcgtcaccattttcctgttttatacgtatagactatatttttatccgatataaaagcagtaaacccttgtttttctgatgcctactatatgctcatggaagttctgctatccggctatgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG

Basic information

species Zea mays
transcript GRMZM2G031572_T02
intron # 10
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G031572_T02 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os10g36760.1 (Oryza sativa), 3'ss of exon 11
gtgagcatttagttctacatgtcacttcgtcaccattttcctgttttatacgtatag-actatatttttatccgatataaaagcagtaaacccttgtttttctgatgcctactatatgctcatggaagttctg-ctatccggctatgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG
|||||||| |     ||||       || |  ||||||   ||||| |     |||| ||| | ||| ||||  |||| |     || ||    |||   |    |   ||    ||  | ||     | |    |||    | |||  | ||||||| |||||| ||||||||||||  || |||||||| ||||||||||| |||||||||||||||||||||||| | |||||||
gtgagcatctg----tacag------tcctggccatttctttgtttca-----atagcactgtttttgtatctcatatgagcatggttaaatagtgtgaatgcattttttatggcattatgatatgcttacaaattatttttcaatgggtacagTGCCGTCATACTTAATTGAGTGCAGCAATTGCGGACATTGCAGTGATCTATCTGGATGCCCTCAAGCTCCTTCACATATTGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76291128|gb|DV030696.1|DV030696
EST:     ATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAAT                         TGCCATCATACTTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCT
genomic: ATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCT
EST: gi|211191456|gb|FL473730.1|FL473730
EST:     ATCTCAAGACCCATGGCGCCACGCTTYTAAGCAGAAATCATCAGACGAAT                         TGCCATCATACTTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCT
genomic: ATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCT
EST: gi|76291129|gb|DV030697.1|DV030697
EST:     ATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAAT                         TGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCT
genomic: ATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCT
EST: gi|32827533|gb|CD967211.1|CD967211
EST:     ATCTCAAGACCCATGGCGCCATGCTTCTAAGCAGAAATCATCAGACGAAT                         TGCCATCATAC
genomic: ATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 119

GTTCTAAAATTGTTTTTGCAAATGGATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 139

GTTCTAAAATTGTTTTTGCAAATGGATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 85

GTTCTAAAATTGTTTTTGCAAATGGATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 82

GTTCTAAAATTGTTTTTGCAAATGGATCTCAAGACCCATGGCGCCACGCTTCTAAGCAGAAATCATCAGACGAATgtgagcattt ... atgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttctgatgcctactatatgctcatggaagttctgctatccggctatgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG
 ttctgat  putative branch site (score: 82)
           tactatat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagcatttagttctacatgtcacttcgtcaccattttcctgttttatacgtatagactatatttttatccgatataaaagcagtaaacccttgtttttctgatgcctactatatgctcatggaagttctgctatccggctatgcctgcagTGCCATCATACCTAATTGAGTGCAAGAACTGCGGACACTGCAGTGATCTCTCTGGATGCCCTCAAGCTCCTTCAAACATTGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG