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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtttagtttgtgaaatggtttaaaagaaattaacagttagaattatgtaggttgtctattcagttctcctactctgatatttatgaaccgtacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG

Basic information

species Zea mays
transcript GRMZM2G020002_T01
intron # 10
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|74240936|gb|DT648850.1|DT648850
EST:     GTC-TGAGGGAACGGATGCATATACTTTGGGACAGCATGGTGGAG                       
genomic: GTCTTGA-GGAACGGATGCATATACTTTGGGACAGCATGGT---Ggtaagtttag ... tacacagg
EST: gi|113703820|gb|EE681324.1|EE681324
EST:     GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGGAG                       
genomic: GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGT---Ggtaagtttag ... tacacagg
EST: gi|211361841|gb|FL432810.1|FL432810
EST:     GAACGGATGCATATACTTTGGGACAGCATGGTG                         ACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG
genomic: GAACGGATGCATATACTTTGGGACAGCATGGTGgtaagtttag ... tacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG
EST: gi|78079711|gb|DV508123.1|DV508123
EST:     GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGGAG                       
genomic: GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGT---Ggtaagtttag ... tacacagg
EST: gi|148929734|gb|EC876616.2|EC876616
EST:     GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGGAG                       
genomic: GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGT---Ggtaagtttag ... tacacagg

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 34

GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGgtaagtttag ... tacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 134

GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGgtaagtttag ... tacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 97

GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGgtaagtttag ... tacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 78

GTCTTGAGGAACGGATGCATATACTTTGGGACAGCATGGTGgtaagtttag ... tacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtaggttgtctattcagttctcctactctgatatttatgaaccgtacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG
                          ctctgat  putative branch site (score: 78)
                               atatttatgaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtttagtttgtgaaatggtttaaaagaaattaacagttagaattatgtaggttgtctattcagttctcctactctgatatttatgaaccgtacacaggagACTGGCAAGATTTCTGTAACCGACTATGTCAGAGTGACAAGCACAGAATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGA