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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtagcgaacacactccgacactcgccacccccctctcgcccccttcgcccgcagctgccgtcgcatgaaattgacgaactctgcttcctcttctcgatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG

Basic information

species Zea mays
transcript GRMZM5G860137_T01
intron # 1
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211416631|gb|FL428585.1|FL428585
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211434558|gb|FL418498.1|FL418498
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|71298333|gb|DR785148.1|DR785148
EST:     CGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCCCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCACCTTC
genomic: CGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|89756408|gb|DY686458.1|DY686458
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|50337630|gb|CO532756.1|CO532756
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|149110543|gb|EE190473.2|EE190473
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|89760643|gb|DY688974.1|DY688974
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|149071725|gb|EE160115.2|EE160115
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211456419|gb|FL396787.1|FL396787
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|149000558|gb|EE037748.2|EE037748
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211023121|gb|FL428611.1|FL428611
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211376449|gb|FL382306.1|FL382306
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTKAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211245222|gb|FL396978.1|FL396978
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGAKGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211024306|gb|FL368563.1|FL368563
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|149029957|gb|EE286286.2|EE286286
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|37419864|gb|CF647618.1|CF647618
EST:     TCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: TCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211136636|gb|FL389507.1|FL389507
EST:     CCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|149087112|gb|EE176651.2|EE176651
EST:     GTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|74232957|gb|DT640871.1|DT640871
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|71426143|gb|DR807193.1|DR807193
EST:     CGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211158239|gb|FL401295.1|FL401295
EST:     GCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211199171|gb|FL385134.1|FL385134
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|78087866|gb|DV516259.1|DV516259
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|78078473|gb|DV506886.1|DV506886
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|89248149|gb|DY619935.1|DY619935
EST:     CAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|148947617|gb|EC893097.2|EC893097
EST:     CGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|149084568|gb|EE174037.2|EE174037
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211111975|gb|FL386052.1|FL386052
EST:     ACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: ACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|78085615|gb|DV514008.1|DV514008
EST:     CAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|71442414|gb|DR823464.1|DR823464
EST:     CGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|93016086|gb|EB641606.1|EB641606
EST:     CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: CGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211164630|gb|FL401351.1|FL401351
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|89248499|gb|DY620285.1|DY620285
EST:     GGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|71439486|gb|DR820536.1|DR820536
EST:     GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
EST: gi|211403233|gb|FL400076.1|FL400076
EST:     GTACTCGGAGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTG                         CTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC
genomic: GTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 36

GGTCGAGCCTCTAGAGAAGCGAGCCGTGCCCGTCTCTCCCAATCCACTCCGTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 234

GGTCGAGCCTCTAGAGAAGCGAGCCGTGCCCGTCTCTCCCAATCCACTCCGTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 86

GGTCGAGCCTCTAGAGAAGCGAGCCGTGCCCGTCTCTCCCAATCCACTCCGTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG


Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 74

GGTCGAGCCTCTAGAGAAGCGAGCCGTGCCCGTCTCTCCCAATCCACTCCGTACTCGGCGCTCCTCCCCCGTCGCCGCCGGAAATCAGGAAGCCCAAGTGgtagcgaaca ... gatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gcagctgccgtcgcatgaaattgacgaactctgcttcctcttctcgatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG
                  aattgac  putative branch site (score: 74)
                            ctctgcttcctcttct  putative PPT
              atgaaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtagcgaacacactccgacactcgccacccccctctcgcccccttcgcccgcagctgccgtcgcatgaaattgacgaactctgcttcctcttctcgatctcgcagCTCTCTTGGGTGCGCTCGTCGCCGGTGGCAGTGAGCCAGTGATGCATCTTCATCTCCGGCTTCCTTATTAGGATCAGCCCGTCGATCGATCATGGCGTCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -CGTCGCCG