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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...cttgcattgctggttttcatttttaacattattcttccatgctcatcagtgaaaaaaattgcttgatgtggatgtctgatatgtttttattgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA

Basic information

species Zea mays
transcript GRMZM5G827505_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G827505_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os01g51390.1 (Oryza sativa), 3'ss of exon 1
------cttgcattgctggttt-tcatttttaacatt--attcttccatgctcatcagtgaaaaaaattgcttgatgtggatgtctgatatgtttttattgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA
      |  |      | | || |||  | ||| ||   ||   |  ||  |  |  ||| |      |||||||| ||     || | |||| ||||| |  | | |||||||||| |||||||| |||||||||||||||||||||||||| |||||||||||||| ||||| || || ||||||||||||||||||| ||| ||||
ctcgatcccgtgcaattagcttatcagatctaatatacaatatatatattgtgtttcgtgga------tgcttgatctgtg---cttacatgtgtttatggttagacagGTGCAAGGAATGATGGACGAGGTGATATTTGATCACTTGCATGCCGCAGCATTCCAAGGTCATCCGCTGGGTGACACAATATTAGGTCCTGTAGAGAATA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148945033|gb|EC890217.2|EC890217
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|91055519|gb|EB165937.1|EB165937
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAA                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGC
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGC
EST: gi|148983190|gb|EE029136.2|EE029136
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|101389579|gb|EB816722.1|EB816722
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCAT
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCAT
EST: gi|71430235|gb|DR811285.1|DR811285
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|149009597|gb|EE041558.2|EE041558
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|74244154|gb|DT652068.1|DT652068
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|148959580|gb|EC905539.2|EC905539
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAACA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|91056228|gb|EB166646.1|EB166646
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|71436989|gb|DR818039.1|DR818039
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
EST: gi|149078045|gb|EE166785.2|EE166785
EST:     AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAG                         GTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA
genomic: AAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 44

GGCGGCACTCGACGTCCTCAGCGACATCCTACAGCACCCGCGATTCCCCGAAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA


Block sizes: 43 (upstream exon), 49 (downstream exon)
Score: 44

GGCGGCACTCGACGTCCTCAGCGACATCCTACAGCACCCGCGATTCCCCGAAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 38

GGCGGCACTCGACGTCCTCAGCGACATCCTACAGCACCCGCGATTCCCCGAAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 41

GGCGGCACTCGACGTCCTCAGCGACATCCTACAGCACCCGCGATTCCCCGAAAGGGCTATCCAGCGGGAGCGCGGCGTCATCCTCCGTGAGATGGAGGAGgtgagctttt ... tgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcagtgaaaaaaattgcttgatgtggatgtctgatatgtttttattgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA
                            gtctgat  putative branch site (score: 41)
                                      tttttatt  putative PPT
                               tgatatgtttttattg  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cttgcattgctggttttcatttttaacattattcttccatgctcatcagtgaaaaaaattgcttgatgtggatgtctgatatgtttttattgaaaaatagGTGCAAGGTATGATGGAAGAGGTGATATTTGATCACTTGCATGCAGCAGCATTCCAAGGCCATCCACTAGGGGACACAATATTAGGTCCTGAAGAAAATA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA
- - -attgctg
- - - - - - - tttcatt
- - - - - - - - - - - - - - - - - -tccatgc
- - - - - - - - - - - - - - - - - - - - - - - - - -aaaaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgcttga
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gtggatg