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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gctttgtgtgcagtatatttcgtttcttctacaagcactattatctaaccacttttttttttttacttatttggactctattcgtttgttgctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG

Basic information

species Zea mays
transcript GRMZM5G809292_T02
intron # 1
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71301886|gb|DR787012.1|DR787012
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|76281110|gb|DV020678.1|DV020678
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|71766161|gb|DR964098.1|DR964098
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|211491318|gb|FL191820.1|FL191820
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|211491317|gb|FL191819.1|FL191819
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|78087661|gb|DV516054.1|DV516054
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         ----ATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|76916193|gb|DV166281.1|DV166281
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|32865447|gb|CF005129.1|CF005129
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGAGGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
EST: gi|101398323|gb|EB821034.1|EB821034
EST:     CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTT                         GCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
genomic: CGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 28

TCTGGGCCTCCTCCCGCCGCGCCACGCCGCACCGGGGTCGTCTCTCTTCCCGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 122

TCTGGGCCTCCTCCCGCCGCGCCACGCCGCACCGGGGTCGTCTCTCTTCCCGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 15

TCTGGGCCTCCTCCCGCCGCGCCACGCCGCACCGGGGTCGTCTCTCTTCCCGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 23

TCTGGGCCTCCTCCCGCCGCGCCACGCCGCACCGGGGTCGTCTCTCTTCCCGGCCGTTGCCCACCGCCCCTTTGCTGGAACCACCATCCATTTCTTCCTTgtaagttgtc ... gctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

taaccacttttttttttttacttatttggactctattcgtttgttgctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG
 atctaac  putative branch site (score: 23)
     acttttttttttttac  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gctttgtgtgcagtatatttcgtttcttctacaagcactattatctaaccacttttttttttttacttatttggactctattcgtttgttgctaaggaagGCAGATCATCAAAGAGAGGATGGGTCGTTTTTGCTTCAGAAAACGTACAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGATCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGATG
- - - tgtgcag
- - - - - - - - - - - - - - - -caagcac