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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtctgtgaacctgccatctagaagccactgtcgtttcttaaaattttgattgttttggcagctgctctaaaaattcctccttttgattattctttgacctggaaccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA

Basic information

species Zea mays
transcript GRMZM2G398500_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G398500_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os02g43710.1 (Oryza sativa), 3'ss of exon 1
gtctgtgaacctgccatctagaagccactgtcgtttcttaaaattttgattgttttggcagctgctctaaaaattcctccttttgattattctttgacctggaaccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA
         |||     ||| ||    |||  |  || |    ||||  ||  |     || ||||||||||||||  |||| |     ||| |  |  ||          |||  ||| ||||||||||||||||||| ||||| |||||||||||||| || |||||    |||||| |||||||| | |   | |||||| || |||| ||
--------gcct-ttgccta-aattttctgaagaatcct----tttttttttctgaaagagttgctctaaaaattctcccttgttgcggttcctaaatttgtgggtggaaacagAAATCGTAGAGCTGAGGCTGGAGCGTCCGGAGGTCAAGAACGCCATCAACTGGGAAGCCATGAGGCTGCTGCGGGGTGTGGTCGAGAAGGTTGAGGCTGA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211224733|gb|FL338371.1|FL338371
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|74232150|gb|DT640064.1|DT640064
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|211446507|gb|FL092865.1|FL092865
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|89756234|gb|DY686335.1|DY686335
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|211253387|gb|FL355069.1|FL355069
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|211446509|gb|FL092867.1|FL092867
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|211522191|gb|FL317001.1|FL317001
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|211078098|gb|FL332537.1|FL332537
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|74244449|gb|DT652363.1|DT652363
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|71439182|gb|DR820232.1|DR820232
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|5566932|gb|AI881843.1|AI881843
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAACGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|101400934|gb|EB822151.1|EB822151
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
EST: gi|78110970|gb|DV529368.1|DV529368
EST:     AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCG                         GGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT
genomic: AGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 49

GTGTCCACCACTGGCCACAACTCCCTACTCGCTCGCACCTTCCAAATCCTAGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 86

GTGTCCACCACTGGCCACAACTCCCTACTCGCTCGCACCTTCCAAATCCTAGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 34

GTGTCCACCACTGGCCACAACTCCCTACTCGCTCGCACCTTCCAAATCCTAGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 78

GTGTCCACCACTGGCCACAACTCCCTACTCGCTCGCACCTTCCAAATCCTAGCCCAGCCGGAGTCCGTCCGTCTGCAGAAGCACCCGGCACCCAACTCCGgtctgtgaac ... ccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agctgctctaaaaattcctccttttgattattctttgacctggaaccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA
                                             ccccgttc  CT-rich tract
                     ttttgattattcttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtctgtgaacctgccatctagaagccactgtcgtttcttaaaattttgattgttttggcagctgctctaaaaattcctccttttgattattctttgacctggaaccccgttcagGGATCTTAGAGCTGAGGCTGGAGCGGCCGGAAGTCAAGAACGCCATTAATTGGGATATGATGAGGATGCTGCGGAGCGCCATAGAGAAGATTCAGGCCGA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC