ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...cctaatgcttcattgggcgcgcaagatgtttttgttgttgtttctggatgtgcaggggattttaggactgacaggttgtatttatcaatctcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCGAAGAGGTTGGCCCAGTCGTATCCTTCAG

Basic information

species	Zea mays
transcript	GRMZM2G174919_T02
intron #	1
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G174919_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os11g07490.1 (Oryza sativa), 3'ss of exon 1

cctaatgcttcattgggcgcgcaagatgtttttgt-tgttgttt-ctggatgtgcagg--ggattttaggactgacaggttgtatttatcaatctcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCGAAGAGGTTGGCCCAGTCGTATCCTTCAG
    | | || | ||   |  |||  || ||| || |   |||| |||   ||||      ||||   |||       ||||  ||  | ||| |   || |||| || ||||| || ||||||||||| |||||||||||||||||||||||||| || || ||||| || || || |||||
---gagggtttagtgtttgaccaatttggtttggtctagggtttactgctcgtgccctttcgattgacggatgcgtttgttg-gttgttgaattttggttgcagTCGGGAACATTCCTTACGATGCGACGGAGGAGCAGCTCGTGCAGATTTGCGAGGAAGTCGGCCCTGTGGTCTCATTCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211229884|gb|FL170973.1|FL170973

EST:     GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCG                         TTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG
genomic: GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG

EST: gi|211231934|gb|FL170974.1|FL170974

EST:     GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCG                         TTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG
genomic: GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG

EST: gi|161589532|gb|EY957879.1|EY957879

EST:     GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCG                         TTGGCAACATACCATACGATG
genomic: GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATG

EST: gi|211229883|gb|FL170972.1|FL170972

EST:     GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCG                         TTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG
genomic: GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG

EST: gi|31360834|gb|CD445191.1|CD445191

EST:     GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCG                         TTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG
genomic: GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG

EST: gi|16916231|gb|BM072752.1|BM072752

EST:     GTTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCG                         TTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG
genomic: GCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 2 RNA-Seq libraries.

Block sizes: 43 (upstream exon), 23 (downstream exon)
Score: 3

TCTCACGGCGGAAGCAGCCAAGCAGCCGGCCGGGCGCCTCCCGTGCCGCCGCTGAGCATGGCCGCTGCTCCCGTGGGCGCTCAGAATCGCTGCGTCTTCGgtacgccttc ... tcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCGAAGAGGTTGGCCCAGTCGTATCCTTCAG

Block sizes: 15 (upstream exon), 35 (downstream exon)
Score: 2

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ggatgtgcaggggattttaggactgacaggttgtatttatcaatctcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCGAAGAGGTTGGCCCAGTCGTATCCTTCAG

                    gactgac  putative branch site (score: 2)
                              ttgtatttatcaat  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cctaatgcttcattgggcgcgcaagatgtttttgttgttgtttctggatgtgcaggggattttaggactgacaggttgtatttatcaatctcaattacagTTGGCAACATACCATACGATGCGACAGAGGAGCAGCTCGTGCAGATTTGCGAAGAGGTTGGCCCAGTCGTATCCTTCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGG
- - -tgcttca
- - - - - - - - - - - - - - - - tgttgtt
- - - - - - - - - - - - - - - - - - - - - - - - -gtgcagg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgtattt