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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtccgtgccgcgccacgcccccgcgcagatcgggggtggatcggatctcctgttggggtccgctgggctagcgttagggttttatttgatcggttcgttctacggattgatcctggctgtggattgtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG

Basic information

species Zea mays
transcript GRMZM2G169095_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G169095_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os11g33330.1 (Oryza sativa), 3'ss of exon 1
gtccgtgccgc-gccacgccc----ccgcg------cagatcgg-gggtggatcggatctcctgttggggtccgctgggctagcgttagggtttt----atttgatcggttcgttctacggattgatcctggctgtggattgtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG
||| || |||| ||| | |||    |||||       ||||||| ||| |    ||||     | |    ||| |  |  | |  |   |||  |    || |  | | ||     |  ||  || ||   ||||||   || | ||||||||||||||||||||||||| ||| || |||||| ||||| ||||||||||||| |||||||| |
gtcagttccgccgccgccccctcctccgcggcggactagatcggtgggggtgctggattctgcgatccc-tcctcctgtttggtttgttggtcgtggtgatgttgttgattgaggatttggtgtggtctgtgctgtgctgtgct-gtgcagAGCGAGTACGTGTCGGAGCGGGACAAGCGGCGGCAGTTCGTCTCCGGGTTCACCGGGAGCGCCG

upper sequence: GRMZM2G169095_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os06g45360.1 (Oryza sativa), 3'ss of exon 1
----gtccgtgccgcgccacgcccccgcgcaga-tcg---ggggtggatcggatctc--ctgttggggtccgctgggctagcgttagggttttatttgatcggttcgttctacggat--tgatc------ctggctgtgg---------------attgtttg--tgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG
    ||||| ||| |||  |  || | ||| || |||    |    ||||||   |    | |||  ||       | | |  ||   |||||    ||||  ||| | ||   |||  || |        ||| |||||               |||| |||  ||||||||||||| |||||||||| ||| || |||||| ||||| ||||||| ||||| ||||||||||
gtccgtccgcgcc-cgctgccacctcccgcggagtcgatcagatcagatcgggcttggatttttgttgttgttgttgttggattttccgttttgcgcgatctcttcttcctctcgatgatggttgaggatttgggtgtggttttgtgggtgattgattgattgattgcagAGCGAGTATGTGTCGGAGCGGGACAAGCGGCGGCAGTTCGTCTCCGGCTTCACCGGGAGCGCTG

upper sequence: GRMZM2G169095_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os11g33340.1 (Oryza sativa), 3'ss of exon 1
----------------------------------------------gtccgtgccgcgccacgcccccgcgcagatcgggggtggatcggatctcctgttggggtccgc----tgggctagcgttagggt--tttatttgatcggttcgttctacggattgatcctggctgtggattgtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG---------
                                              |  || | || | |  |        |    ||   | |   |   | |  ||||||  |  ||    |  |||| |  |       ||  |||  | ||||||| ||   ||||||   | |  | |  ||||  |||||||||||||| ||| |||||| ||| || |||| | ||||||||||||||||||| |||| |||           
gtctgctcttggcgccgcctcgtccgcggaatggggggaggcgattgatcg-gtcgggtcgggattagatccgatccgaccgagctccattttttttgttggattttgcgttttcagctatctctcctcctgttggttttgttggttcgtgctggtgattgaggattggcgcgatttgtgggtgcagAGCGAGTATGTGGCGGAGCGGGACAAGCGGCTGCAGTTCATCTCCGGGTTCACCGGGATCGCCCGCAGCGCTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211311977|gb|FK981217.1|FK981217
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|211311973|gb|FK981213.1|FK981213
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|148948656|gb|EC898570.2|EC898570
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|21987275|gb|BQ778803.1|BQ778803
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|211311976|gb|FK981216.1|FK981216
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|211311972|gb|FK981212.1|FK981212
EST:     ACTYKCCGCCGCTCSACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTAYGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|78113783|gb|DV532172.1|DV532172
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|211311975|gb|FK981215.1|FK981215
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|149085325|gb|EE174831.2|EE174831
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
EST: gi|211311974|gb|FK981214.1|FK981214
EST:     ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAG                         AGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC
genomic: ACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 258

GTCGGCGAGGGACGAGCATCTTGATGGGATTCGCGCGCTGATGGCGGCCCACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 225

GTCGGCGAGGGACGAGCATCTTGATGGGATTCGCGCGCTGATGGCGGCCCACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 99

GTCGGCGAGGGACGAGCATCTTGATGGGATTCGCGCGCTGATGGCGGCCCACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 305

GTCGGCGAGGGACGAGCATCTTGATGGGATTCGCGCGCTGATGGCGGCCCACTCGCCGCCGCTCCACGGGCTGGTCGTCCCCTCCGAGGATGCCCACCAGgtccgtgccg ... gtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttatttgatcggttcgttctacggattgatcctggctgtggattgtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG
                        gattgat  putative branch site (score: 305)
                                          attgttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtccgtgccgcgccacgcccccgcgcagatcgggggtggatcggatctcctgttggggtccgctgggctagcgttagggttttatttgatcggttcgttctacggattgatcctggctgtggattgtttgtgcagAGCGAGTACGTGTCGGAGCAGGATAAACGGCGGGAGTTCATCTCCGGGTTCACAGGGAGCGCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCAG