ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

gtaagcagcgcccgcccgggtccctcttcccgatctgtgccgttcgcgttagtggtggagccaaggtttccgggatctattcggagggttccctgttcgtgcgggcgggctaagcggttgggttctgtgcgagtccctgaaaccagtggtcgacttcgtggtctcagGCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGCGCCGTGCCGATGCACAAGGTCAACTTCGACGCCAAGACGGAGTACGACA

Basic information

species	Zea mays
transcript	GRMZM2G163409_T01
intron #	1
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G163409_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os10g35580.1 (Oryza sativa), 3'ss of exon 1

gtaagcagcgcccgcccgggtccctcttcccgatctgtgccgttcgcgttagtggtggagccaaggtttccgggatctattcggagggttccctgttcgtgcgggcgggctaagcggttgggttctgtgcgagtccctgaaaccagtggtcgacttcgtggtctcagGCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGCGCCGTGCCGATGCACAAGGTCAACTTCGACGCCAAGACGGAGTACGACA
||||   |||| |  ||   |||| |||   |   | ||   |||| ||| || ||       |  | |    |  |    ||  |  ||   | |||  |      |     ||| || | ||      | ||              || |  ||||||     |||| ||||| || ||||| |||||||| ||||||||||||||||| || || || |||||||||||||||||||||||||||||||||||||||||||| |
gtaaatcgcgctcttcc---tccccctt---gggttttg--attcg-gttggttgtttgttggatttcttgatgcgcggggcgtggattttttttttctcggttcttgatgtggcgatttgattt-----gggt--------------gtgggtttcgtg----tagGCTGCGTCGGGGGCGGTGGCGTGCCAGCTGATGGACGCGGCGCACCCGGGGGCGGTGCCGATGCACAAGGTCAACTTCGACGCCAAGACGGAGTACGAGA

upper sequence: GRMZM2G163409_T02 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA01G39720.1 (Glycine max), 3'ss of exon 1

gtaagcagcgcccgcccgggtccctcttcccgatctgtgccgttcgcgttagtggtggagccaaggtttccgggatctattcggagggttccctgttcgtgcgggcgggctaagcggttgggttctgtgcgagtccctgaaaccagtggtcgacttcgtggtctcagGCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGCGCCGTGCCGATGCACAAGGTCAACTTCGACGCCAAGACGGAGTACGACA
                                                                | | |   |   |||||        || |  ||   |        |  | || |||  ||||       ||    |      |||    | |  |  ||||||||    || |||||||||   ||||| ||  | || || || ||||| ||   |||||| |||||||||||||| |||||||||||||  ||||| || |
----------------------------------------------------------------gttatttcgatcctatt--------tctccatttaggtttaatttccgatcg-ttgatttcttactagatcttctattttcttggat--cgttatactctcagGCATGTTCCGGCGCGGTTCACTGCCAGCTTCTCGATGCCGCTCATCCAGGGATCGTGCCTATGCACAAGGTCAATTTCGACGCCAAGAGCGAGTATGAGA

upper sequence: GRMZM2G163409_T02 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA11G05560.1 (Glycine max), 3'ss of exon 1

gtaagcagcgcccgcccgggtccctcttcccgatctgtgccgttcgcgttagtggtggagccaaggtttccgggatctattcggagggttccctgttcgtgcgggcgggctaagcggttgggttctgtgcgagtccctgaaaccagtggtcgacttcgtggtctcagGCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGCGCCGTGCCGATGCACAAGGTCAACTTCGACGCCAAGACGGAGTACGACA
                                             |  ||| |  | || || |  | |||   |   ||||     | |    ||| |||        |  | || |  | ||   | |  | || |  |     | | |  | |  |  | ||||||    || ||||| |||   || || ||  | || || || ||||| ||   |||||| |||||||||||||| |||||||||||||  |||||||| |
---------------------------------------------gttttattt-tcgatcctatttctccatcaa--attcaatcaggtttaggtttgtgtttaatttccgatcgat--gatttcttactggaccttctattttcttg-catcgttatactttcagGCTTGTTCCGGCGCCGTTCATTGTCAGCTTCTCGATGCCGCTCATCCAGGGATCGTGCCTATGCACAAGGTCAATTTCGACGCCAAGAGCGAGTACGAGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|26558995|gb|CA831230.1|CA831230

EST:     CGCGTGGGATCAACACTACGCTGCACCTCGGCCTCTCCAAGGTGGAGGAG                         GCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGC
genomic: CGCGT-GGATCAACACTACGCTGCAGCTCGGCCTCTCCAAGGTGGAGGAGgtaagcagcg ... gtggtctcagGCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 356

CAACATCGGGATGATGGACGGAGCCTACTTCGTCGGGCGCAACGAGATCCTCGCGTGGATCAACACTACGCTGCAGCTCGGCCTCTCCAAGGTGGAGGAGgtaagcagcg ... gtggtctcagGCGGCGTCAGGCGCGGTTGCGTGCCAACTGATGGACGCGGCGCATCCCGGCGCCGTGCCGATGCACAAGGTCAACTTCGACGCCAAGACGGAGTACGACA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 37

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 33