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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtattgcaagttttagcaggccacgcccccgccgcgttcttggcttgtgttttagcagacaagttaatgcctgcgtgatcggtgctctgcttgttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG

Basic information

species Zea mays
transcript GRMZM2G162531_T03
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G162531_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os06g42800.1 (Oryza sativa), 3'ss of exon 1
gtattgcaagttttagcaggccacgcc--cccgccgcgttcttggcttgtgttttagcagacaagtta-atgcctgcgtgatcggtgctctgctt---gttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG
||| |   | ||  ||||   ||| ||        |  |||||||  || ||     | |     | | ||| ||||  |   | |    |||||   |||||||||| ||||||  |||||||||| |||  ||||||||| |||||||| ||||||||||||||||| ||| ||||||||||||| ||||| || ||||||  |||
gtaatatcaattgcagcat-tcaccccaagttcttggtttcttggaatgcgtgcgtactgtttgatgatatgtctgcacggatgatcagttgcttttggttcttgcagCCGCGGCGCTGGGGAATCTGTACTCCTCCTGGAATAGCCCGGCTCAGCTCACCGGCTGGTCGGCCGGCGGCGGCGACCCTTGCGGTGCCGCGTGGATGGG
















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 41 (downstream exon)
Score: 20

ATGAAGTCGAGAACTGCGACATTGGCGGTCGGCATCCTCCTCCTCGTCGCGCTTGTGGTGGTGGCGGAAGCCGACACCGACGCCGGCGATGgtattgcaag ... gttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG


Block sizes: 44 (upstream exon), 47 (downstream exon)
Score: 12

ATGAAGTCGAGAACTGCGACATTGGCGGTCGGCATCCTCCTCCTCGTCGCGCTTGTGGTGGTGGCGGAAGCCGACACCGACGCCGGCGATGgtattgcaag ... gttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG


Block sizes: 27 (upstream exon), 29 (downstream exon)
Score: 2

ATGAAGTCGAGAACTGCGACATTGGCGGTCGGCATCCTCCTCCTCGTCGCGCTTGTGGTGGTGGCGGAAGCCGACACCGACGCCGGCGATGgtattgcaag ... gttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 142

ATGAAGTCGAGAACTGCGACATTGGCGGTCGGCATCCTCCTCCTCGTCGCGCTTGTGGTGGTGGCGGAAGCCGACACCGACGCCGGCGATGgtattgcaag ... gttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgttttagcagacaagttaatgcctgcgtgatcggtgctctgcttgttcttgcagTCGCGGCCTTGGGGAATCTCTACAGCTCCTGGAACAGCCCGGCGCAGCTCACCGGCTGGTCCGCCAGCGGCGGCGACCCCTGCGGCGCTGCGTGGGCGGG
              agttaat  putative branch site (score: 142)
                                     ctctgcttgttctt  putative PPT
             aagttaat  TA-rich tract