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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...aatacacgatgggtagatgcaaagattcaccacgttcttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT

Basic information

species Zea mays
transcript GRMZM2G143469_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os01g40710.1 (Oryza sativa), 3'ss of exon 1
aatacacgatgggtag---atgcaaagattcaccacgttcttgagtaggttaaggaa-gctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
|||| | |  ||||     |  |  || |||      || |||| |||| ||||    |||  | |      | | |||| | | | |     ||| || |||| ||||| ||||||||||||||||||||||||||||||||||||||||| ||||| ||||||||||| ||| ||||||||||||| |||||||| || |||
aataaaagcagggtgacctagacttaggttcggtgttttgttgaatagggtaagatttgcttgtttactaatggtgattc-tgatcatca---aaatctcgcagATGGGCTATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACCGGCTCGGATTTTGTTCAACAAGTGAAGATCCTTCTCTCCAATTTTGGGATT

upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA20G30940.1 (Glycine max), 3'ss of exon 1
aatacacgatgggtagatgcaaagattcaccacgttcttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctacaaacctt-------gcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
   | | |||   |   |     | ||||     |   ||  ||    | |  || ||  ||||      | |||   |   ||  |    || ||        ||| ||||| |||||||| ||||| || ||  ||||||||||||| |||||||| || ||||||||| | ||| | ||||||||||| || ||||| || || 
---atatgataccttagtt----gtttcattgaatatgtgcatactcaatgctagttctgctctgcctatttatttgactttttaatctggacttttgtttcaacagATGGGCTATGTTTGGTTTTGCTGTTGGATTGCTAACAGAGTATGCAACAGGATCAGATTTTGTTGATCAAGTAAAGATCCTTCTATCAAATTTTGGGATA

upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA16G27850.1 (Glycine max), 3'ss of exon 1
----aatacacgatggg-tagatgcaaagattcaccacgttcttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
      | || | |  | ||| |    |  ||       || |    | ||||       ||  | | | |   || | | |||||   | |   |    | ||| ||||| |||||||| ||||| |||||| ||||||||||||| |||||||| || || |||||| | ||| ||||||||||||| || ||||| || || 
ttgttgtgcaagttctgctagttttttattttttttttatttt---atcggttgcaagttctgctgtattttgacttactaatcagtaatttc--atttctacagATGGGCAATGTTTGGTTTTGCTGTAGGGTTGCTAACAGAGTATGCAACAGGTTCAGACTTTGTTGATCAAGTGAAGATCCTTCTATCGAATTTTGGGATA

upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA02G08740.1 (Glycine max), 3'ss of exon 1
---aatacacgatgggtagatgcaaagattcaccacgttcttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctaca--aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
     ||     ||  ||  ||  |   || |     | |        |||  | ||| ||     | |    | | | |  |   |   |    |    | ||| ||||| |||||||| ||||| || ||| ||||||||||||| |||||||| || || |||||| | ||| | ||||||||||| || ||||| ||||| 
catcctatttattgcatacctgtcacacttaattgaatcccca---ttgttgtgcaagttc--tgatgtattttgacttactaatatgtaatttcatttctacagATGGGCAATGTTTGGTTTTGCTGTTGGGTTGCTAACAGAGTATGCAACAGGTTCGGACTTTGTTGATCAAGTAAAGATCCTTCTATCGAATTTTGGAATA

upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA10G36650.1 (Glycine max), 3'ss of exon 1
aatacacgatgggtagatgcaaagattcaccacgttcttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctacaaacctt--gcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
  |  | ||    |          ||  |  | || | |   || | ||     ||||  |  | |    | | |  | |   |   |      ||   ||| ||||| |||||||| ||||| || ||  ||||||||||||| ||||| || || ||||||||| | ||| | ||||||||||| || ||||| ||||| 
--tttatgacaccttagctttttcatggaatatgtgcatatttatgctagttctgctctgcctttttatttgactttttaatctggaattttgtttcaacagATGGGCAATGTTTGGTTTTGCTGTTGGATTGCTAACAGAGTATGCAACGGGATCAGATTTTGTTGATCAAGTAAAGATCCTTCTCTCGAATTTTGGAATA

upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: AT1G34000.1 (Arabidopsis thaliana), 3'ss of exon 1
aatacacgatgggt-agatgcaaagattcaccacgttc--ttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
 ||  | | |   | | || || | ||  |   | |    ||    |  |    |  | |  |  |||  |   |  | |    || | |       ||| |||||||| |||||||| || ||||| || ||||| |||||||| |||||||| || ||  ||||| | ||| | ||||||||||| || |||||||| |||
-attgatgtttcttcaaattcagacatatatagcctaaagtttcacacttgtcagttggtactgcctagacaagagataact--ctgtgtgttttgtttgtagGTGGGCTATGTTTGGTTTCGCAGTTGGTATGCTCACAGAGTATGCAACAGGATCAGACCTTGTTGATCAAGTTAAGATCCTTCTCTCAAATTTCGGCATT

upper sequence: GRMZM2G143469_T01 (Zea mays), 3'ss of exon 1
lower sequence: Vv18s0072g00120.t01 (Vitis vinifera), 3'ss of exon 1
aatacacgatgggtagatgcaaagattcaccacgttcttgagtaggttaagga---agctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
  | |   ||    |  |   | | |   | |  ||  ||  |   || |  |   |    | ||   | |     |   ||    |     |       ||| ||||| ||||| || ||||| || |||||||||||||||||||||||||||||||| |||||| | ||| | ||||||||||| |||||||| || || 
--tcctttataaacat-tattatgttggcctatattaatgtatttcttcatcagccaatctagcttcttacttgggtctgtctctgtctgggatgttaatcagATGGGCAATGTTCGGTTTTGCTGTTGGGATGCTAACAGAGTACGCAACAGGCTCTGACTTTGTTGATCAAGTAAAGATCCTTCTCTCCAATTTTGGGATA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211334184|gb|FL266782.1|FL266782
EST:     CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
genomic: CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
EST: gi|211332029|gb|FL266778.1|FL266778
EST:     CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
genomic: CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
EST: gi|211334188|gb|FL266786.1|FL266786
EST:     CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
genomic: CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
EST: gi|28983103|gb|BQ539447.2|BQ539447
EST:     CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTACAATAGGGATGCTAACAGAGTACGCAACAGG
genomic: CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG
EST: gi|211334187|gb|FL266785.1|FL266785
EST:     CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAG                         GTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACACG
genomic: CAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 3 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 62

CCAAGGACCTGCAAGACTACTTCAAGAAGAAGAAGCTGGACGAGGCCGACCAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT


Block sizes: 24 (upstream exon), 38 (downstream exon)
Score: 4

CCAAGGACCTGCAAGACTACTTCAAGAAGAAGAAGCTGGACGAGGCCGACCAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT


Block sizes: 46 (upstream exon), 43 (downstream exon)
Score: 45

CCAAGGACCTGCAAGACTACTTCAAGAAGAAGAAGCTGGACGAGGCCGACCAGGGCCCATTCTTTGGGTTCGTGCCCAAGAATGAGATTTCCAACGGAAGgtacgttcgc ... aaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gttaaggaagctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT
                      cgctaat  putative branch site (score: 45)
                         taattcat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
aatacacgatgggtagatgcaaagattcaccacgttcttgagtaggttaaggaagctcatctctatgcgctaattcatcagctttctacaaaccttgcagGTGGGCCATGTTTGGGTTTGCAGTAGGGATGCTAACAGAGTACGCAACAGGCTCTGATTTTGTTCAGCAATTGAAGATCCTTCTTTCCAATTTCGGAATT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - -gatgcaa
- - - - - - - - - - - - - -caccacg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -catcagc