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 5'  3'   		2  
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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...cattatgtcttggttcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG

Basic information

species Zea mays
transcript GRMZM2G142768_T02
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G142768_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os02g57250.2 (Oryza sativa), 3'ss of exon 1
cattatgtcttggt-tcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG---
   ||| | |  |   | ||     ||   |   | | |      ||  || |  ||| |   ||||||| |||    ||||||||||  ||||||   ||||||||||||||||||||||||||||| ||  ||   ||||||||||||||||| || || ||||| ||||| ||  |||| ||  | | | ||||||||   
agatatatatcagcaccagagttgctgagctacacctacacatgtag--acttttcaatac--atttcaactaatcccaactgcatttcctggtttggcagcagCTTTGGAGTGGTGGGATGGCCACCGATCCGACAGTTCAGGATGAACAGCCTGTTCAATCAGGCTAAAGAAAACACATCTGAAACCGACACCAAGAAGACC

upper sequence: GRMZM2G142768_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os02g57250.1 (Oryza sativa), 3'ss of exon 1
cattatgtcttggttcggaagaaatgtcattttct-tgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG
 |  |  | |   |  | |  | |  |  |   ||   | | |  |  ||        |||  ||||||| |||    ||||||||||  ||||||   ||||||||||||||||||||||||||||| ||  ||   ||||||||||||||||| || || ||||| ||||| ||  |||| ||  | | | ||||||||
-accagatatatatcagcaccagagttgctgagctacacctacacatgtagacttttcaatacatttcaactaatcccaactgcatttcctggtttggcagCAGCTTTGGAGTGGTGGGATGGCCACCGATCCGACAGTTCAGGATGAACAGCCTGTTCAATCAGGCTAAAGAAAACACATCTGAAACCGACACCAAGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76018364|gb|DT945534.1|DT945534
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71444561|gb|DR825611.1|DR825611
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211356600|gb|FK980315.1|FK980315
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
EST: gi|211356605|gb|FK980320.1|FK980320
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
EST: gi|71437812|gb|DR818862.1|DR818862
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|149083988|gb|EE173376.2|EE173376
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|91049936|gb|EB160354.1|EB160354
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211356607|gb|FK980322.1|FK980322
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCC
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCC
EST: gi|78108266|gb|DV526684.1|DV526684
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCCGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|50333994|gb|CO529120.1|CO529120
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|76924731|gb|DV170106.1|DV170106
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71418902|gb|DR804585.1|DR804585
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|148961892|gb|EE012679.2|EE012679
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211250491|gb|FK980312.1|FK980312
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGACTGGTGGGATGGCCACCCATAAGAACTTTCA
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCA
EST: gi|93281748|gb|EB674012.1|EB674012
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|78090139|gb|DV518513.1|DV518513
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCAC--AG                         -AGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|148958908|gb|EE010470.2|EE010470
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71301549|gb|DR786822.1|DR786822
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71433960|gb|DR815010.1|DR815010
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71768773|gb|DR966710.1|DR966710
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|76283166|gb|DV022734.1|DV022734
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|91050039|gb|EB160457.1|EB160457
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211356608|gb|FK980323.1|FK980323
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGG-ATGGCCACCCATAAGAACTTTCA
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCA
EST: gi|78114895|gb|DV533283.1|DV533283
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211217174|gb|FL271883.1|FL271883
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|113702671|gb|EE680175.1|EE680175
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCAC--AG                         -AGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211250489|gb|FK980310.1|FK980310
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|76020485|gb|DT947655.1|DT947655
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|78078295|gb|DV506710.1|DV506710
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|149000117|gb|EE037243.2|EE037243
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         C---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|32800222|gb|CD952458.1|CD952458
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 21

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG


Block sizes: 42 (upstream exon), 49 (downstream exon)
Score: 4

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG


Block sizes: 47 (upstream exon), 45 (downstream exon)
Score: 8

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG


Block sizes: 47 (upstream exon), 42 (downstream exon)
Score: 7

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... ggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG
                            cattaac  putative branch site (score: 7)
                                       ttttttg  putative PPT
                                      attttttggttttata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cattatgtcttggttcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagCAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - -gaagaaa
- - - - - - - - - - - - - - - - - -tgcttgc
- - - - - - - - - - - - - - - - - - - - - - - - -cctacca