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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tatgtcttggttcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC

Basic information

species Zea mays
transcript GRMZM2G142768_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G142768_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os02g57250.2 (Oryza sativa), 3'ss of exon 1
---tatgtcttggt-tcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC
   ||| | |  |   | ||     ||   |   | | |      ||  || |  ||| |   ||||||| |||    ||||||||||  ||||||   ||||||||||||||||||||||||||||| ||  ||   ||||||||||||||||| || || ||||| ||||| ||  |||| ||  | | | |||||||| ||
agatatatatcagcaccagagttgctgagctacacctacacatgtag--acttttcaatac--atttcaactaatcccaactgcatttcctggtttggcagcagCTTTGGAGTGGTGGGATGGCCACCGATCCGACAGTTCAGGATGAACAGCCTGTTCAATCAGGCTAAAGAAAACACATCTGAAACCGACACCAAGAAGACC

upper sequence: GRMZM2G142768_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os02g57250.1 (Oryza sativa), 3'ss of exon 1
------tatgtcttggt-tcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC
      ||| | |  |   | ||     ||   |   | | |      ||  || |  ||| |   ||||||| |||    ||||||||||  ||||||   ||||||||||||||||||||||||||||| ||  ||   ||||||||||||||||| || || ||||| ||||| ||  |||| ||  | | | ||||||||   
accagatatatatcagcaccagagttgctgagctacacctacacatgtag--acttttcaatac--atttcaactaatcccaactgcatttcctggtttggcagCAGCTTTGGAGTGGTGGGATGGCCACCGATCCGACAGTTCAGGATGAACAGCCTGTTCAATCAGGCTAAAGAAAACACATCTGAAACCGACACCAAGAAG---
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78078295|gb|DV506710.1|DV506710
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211356607|gb|FK980322.1|FK980322
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCC
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCC
EST: gi|211217174|gb|FL271883.1|FL271883
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|149083988|gb|EE173376.2|EE173376
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|211356602|gb|FK980317.1|FK980317
EST:     ATGCGGCGCCCAACTCGCCAGGGACGGGTCGCGTCTGGC-ACCCACAGAG                         CTTTTGAGGTGGTGGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGC
genomic: ATGCGGCGCCCAACTCGCCAGGGACGG-TCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAG-TGGTGGG-ATGGCCACCCATAAGAACTTTCAGGATGAACAGC
EST: gi|78114895|gb|DV533283.1|DV533283
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|91049936|gb|EB160354.1|EB160354
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71437812|gb|DR818862.1|DR818862
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|211250489|gb|FK980310.1|FK980310
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71301549|gb|DR786822.1|DR786822
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|211250491|gb|FK980312.1|FK980312
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGACTGGTGGGATGGCCACCCATAAGAACTTTCA
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCA
EST: gi|113702671|gb|EE680175.1|EE680175
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|211356600|gb|FK980315.1|FK980315
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
EST: gi|71433960|gb|DR815010.1|DR815010
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|93281748|gb|EB674012.1|EB674012
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|148961892|gb|EE012679.2|EE012679
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|78090139|gb|DV518513.1|DV518513
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|91050039|gb|EB160457.1|EB160457
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|71768773|gb|DR966710.1|DR966710
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|76018364|gb|DT945534.1|DT945534
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|76283166|gb|DV022734.1|DV022734
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|71418902|gb|DR804585.1|DR804585
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|149000117|gb|EE037243.2|EE037243
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|50333994|gb|CO529120.1|CO529120
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|71444561|gb|DR825611.1|DR825611
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|76020485|gb|DT947655.1|DT947655
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|211356608|gb|FK980323.1|FK980323
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGG-ATGGCCACCCATAAGAACTTTCA
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCA
EST: gi|78108266|gb|DV526684.1|DV526684
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCCGGATGAACAGCCT
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCT
EST: gi|211356605|gb|FK980320.1|FK980320
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATG
EST: gi|76924731|gb|DV170106.1|DV170106
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGCAG                       
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCAC--AG-AGgtgagcagca ... tttatagc
EST: gi|32800222|gb|CD952458.1|CD952458
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
EST: gi|148958908|gb|EE010470.2|EE010470
EST:     GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAG                         CAGCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG
genomic: GATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagC---TTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 46 (downstream exon)
Score: 18

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC


Block sizes: 46 (upstream exon), 46 (downstream exon)
Score: 10

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC


Block sizes: 41 (upstream exon), 43 (downstream exon)
Score: 8

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 18

CCCCCGCGGCAGGTGCAGGTACCGCGGCGCCAAGCAAGAGGGCCAAGGCGGATGCGGCGCCCAACTCGCCAGGGACGGTCGCGTCTGGCCACCCACAGAGgtgagcagca ... tttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

acctaccaaaattgatttcaagtaacattaactgcattttttggttttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC
                         cattaac  putative branch site (score: 18)
                                   attttttggttttata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tatgtcttggttcggaagaaatgtcattttcttgcttgccgagttacctaccaaaattgatttcaagtaacattaactgcattttttggttttatagcagCTTTGGAGTGGTGGGATGGCCACCCATAAGAACTTTCAGGATGAACAGCCTATTTAACCAGGCCAAAGAGAATGCATCGGAGGCTGGCGCCAAGAAGCCC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- - - - - - - gaagaaa
- - - - - - - - - - - - - - - - tgcttgc
- - - - - - - - - - - - - - - - - - - - - - - cctacca