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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...taaatttctgctgtctctgtttcatgagcagcacatccttctacatctattgttttctgattaccatactctaagtttggccttgcaattttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG

Basic information

species Zea mays
transcript GRMZM2G137528_T02
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G137528_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os02g11050.1 (Oryza sativa), 3'ss of exon 1
------taaatttctgctgtctctgtttcatgagcagcacatccttctacatctattgttttctgattaccatactctaagtttggccttgcaattttaattt-cagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG
      |  |||| ||  || |||  || |||      | ||  |     |||| ||  ||  | | ||     |||||| | ||    |||||||| |  || ||||||||||||||||||||||| |||||||||||||||| |||||||| ||||||||||||||||||||||||||||| |||||||||||||| ||||||
ctatccttgatttgtggagt-tctacttgatgcatgtgaaatgtt-----atctttttcttctttactatttccctctaaatatg-ttgtgcaatttcatcttgcagTTAGAATGCTCAGGGAAGAGCTGCAGTTGCTTCAAGAACCTGGCTCGTATGTTGGTGAGGTGGTGAAGGTCATGGGTAAATCAAAGGTTCTAGTGAAG

upper sequence: GRMZM2G137528_T02 (Zea mays), 3'ss of exon 1
lower sequence: Vv04s0008g04690.t01 (Vitis vinifera), 3'ss of exon 1
taaatttctgctgtctctgtttcatgagcagcacatccttctacatctattgttttctgatta---ccatactctaagt--ttggccttg-caattttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG
    |  | | ||      |  ||| || |   | |  ||      |||||  || ||    |     | | | | | |  |   |||||    | |||  |||||| || |||||||| ||||| |||||| |||||||||||||||| |||||||||||||| || || || || ||||| ||||| |||||| |||| |||
--ggtcacagttgc----atagcattaggaattcttttttgctttcctattccttactagaaaaattgaaattttgattaatgaaccttggtggtgttacattcagTGAGGATGCTCAGAGAAGAATTGCAGCTGCTTCAAGAGCCTGGATCATATGTTGGTGAAGTAGTCAAAGTTATGGGAAAATCGAAGGTTTTGGTTAAG

upper sequence: GRMZM2G137528_T02 (Zea mays), 3'ss of exon 1
lower sequence: Vv09s0002g05800.t01 (Vitis vinifera), 3'ss of exon 1
---taaatttctgctgtctctgtttcatgagcagcacatccttctacatctattgttttctgattaccatactctaagtttggccttgcaattttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG
    | ||| ||| | |     ||     || |  | |  |||     |||     ||   |  |    ||   |         | |  | |||||||  |||| || ||||| || |||||||||||  | ||||| |||||||| |||||||||||||| || || || || |||||||| ||||||||  | || |||
gaccagattactgttctaagaattcagccagtaataaaatcttgagtttcttcaactt---gggtggtttatgatgttcacatgcatatatttttaatgacagTGAGGATGCTTAGAGAAGAGTTGCAACTTCTTCAGGAGCCTGGATCATATGTTGGTGAAGTTGTCAAAGTTATGGGGAAGTCAAAGGTCTTAGTTAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32828073|gb|CD967751.1|CD967751
EST:     CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAG                         TTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
genomic: CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
EST: gi|211028259|gb|FL437884.1|FL437884
EST:     CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAG                         TTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
genomic: CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
EST: gi|32805431|gb|CD957665.1|CD957665
EST:     CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAG                         TTAGAATGCTCAGGGAGGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
genomic: CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
EST: gi|9742895|gb|BE519043.1|BE519043
EST:     CTATATTCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAG                         TTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
genomic: CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG
EST: gi|32827753|gb|CD967431.1|CD967431
EST:     CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAG                         TTAGAATGCTCAGGGAAGAGTTGCAGTTGCTCCAAGAGCCTGGCTCATATG
genomic: CCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 395

TACTACCTGCAGCACATCCACGACCTGCAGCTCCAGATCCGGCAGAAGACCCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 358

TACTACCTGCAGCACATCCACGACCTGCAGCTCCAGATCCGGCAGAAGACCCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 211

TACTACCTGCAGCACATCCACGACCTGCAGCTCCAGATCCGGCAGAAGACCCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 437

TACTACCTGCAGCACATCCACGACCTGCAGCTCCAGATCCGGCAGAAGACCCACAACCTCAACCGTCTCGAGGCCCAGCGCAATGACCTCAACTCCCGAGgtagcatttc ... ttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tctattgttttctgattaccatactctaagtttggccttgcaattttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG
                                           ttttaat  putative branch site (score: 437)
                                     ttgcaattttaattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
taaatttctgctgtctctgtttcatgagcagcacatccttctacatctattgttttctgattaccatactctaagtttggccttgcaattttaatttcagTTAGAATGCTCAGGGAAGAGTTGCAGTTGCTTCAAGAGCCTGGCTCATATGTTGGTGAGGTGGTGAAGGTCATGGGGAAATCAAAGGTTCTGGTGAAG

- - - tctgctg
- - - - - - - - -tgtttca
- - - - - - - - - - - - - -gcagcac
- - - - - - - - - - - - - - - - - - - - ctacatc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - catactc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ccttgca