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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaccaataccaaccaacacgtcaaattcaccaggcatgccgatttgctagatctgagccgacctgttccgactttgttgtgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAGGAGAGGGACGGCGTCAAGCTGCCAAGCTACAGGGGCGACAACGTCAACG

Basic information

species Zea mays
transcript GRMZM2G117707_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G117707_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os07g42960.1 (Oryza sativa), 3'ss of exon 1
gtaccaata--ccaaccaacacgtcaaattcaccaggcatgccgatttgctagatctgagcc-gacctgttccgactttgttgtgcacggt-agGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAGGAGAGGGACGGCGTCAAGCTGCCAAGCTACAGGGGCGACAACGTCAACG
||||| | |  |||| |  ||   | |  ||| |   || || |||| || |||||||| || || ||| ||| | |||  || ||  | | |||| |||||||||||||||||||| |||||||| ||||| ||  |||| |||||||||||||| || |||||||| || |||||||| |||||| |||| |
gtacccaaaatccaaaccccaaaacgagctcatccttcacgc-gattcgcgagatctgatcccgatctgctccaaattttgtgcgcgagtttagGTCGGGAGGATGGCTGGCCAGTTCGCCAAGCCGAGGTCTGATTCGTTCGAGGAGAGGGACGGGGTTAAGCTGCCGAGTTACAGGGGAGACAACATCAATG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149112304|gb|EE295199.2|EE295199
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148929301|gb|EC875066.2|EC875066
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148986215|gb|EE029963.2|EE029963
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|89754386|gb|DY685168.1|DY685168
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|149109094|gb|EE293133.2|EE293133
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|211222136|gb|FK967457.1|FK967457
EST:     TGCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGGAGGATGGGCTGGC
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGG-AGGATGG-CTGGC
EST: gi|149008651|gb|EE040836.2|EE040836
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|31357672|gb|CD442029.1|CD442029
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGGGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148944292|gb|EC889388.2|EC889388
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148969287|gb|EE020057.2|EE020057
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148939050|gb|EC883443.2|EC883443
EST:     TCCAGGTGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148958081|gb|EC904269.2|EC904269
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148966969|gb|EE018111.2|EE018111
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|71314137|gb|DR793684.1|DR793684
EST:     CCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: CCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|149078125|gb|EE166874.2|EE166874
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148953403|gb|EE152901.2|EE152901
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|149046653|gb|EE048042.2|EE048042
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|149106192|gb|EE291880.2|EE291880
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148928301|gb|EE284920.2|EE284920
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148968299|gb|EE019487.2|EE019487
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCCAG                         GTGGGGAGGATGGCTGGCCCGTTTGCCAAGCCAAGGTCCCAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|37417141|gb|CF646223.1|CF646223
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGC
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGC
EST: gi|149082849|gb|EE172074.2|EE172074
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|37417143|gb|CF646224.1|CF646224
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGC
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGC
EST: gi|88754421|gb|DY538562.1|DY538562
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGC
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGC
EST: gi|149087936|gb|EE287198.2|EE287198
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|76291135|gb|DV030703.1|DV030703
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148931681|gb|EC874867.2|EC874867
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|78108037|gb|DV526455.1|DV526455
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148958869|gb|EE010449.2|EE010449
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAAGGATGGCTGGCCAGTTTGCCA-GCCAAGGTCCGAACCGTTGGA
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGA-GGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGA
EST: gi|148954653|gb|EC900100.2|EC900100
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|29576946|gb|CB617058.1|CB617058
EST:     TCCAGATGGGCGCCG-GCTCATGTTCGGCGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAG-TTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|148968571|gb|EE019575.2|EE019575
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
EST: gi|149075537|gb|EE164067.2|EE164067
EST:     TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAG                         GTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG
genomic: TCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 3 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 60

CTTCAAGGAGTTCCACGCCAACAACATCCGTGACACCTTCCGTATCCTGCTCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAGGAGAGGGACGGCGTCAAGCTGCCAAGCTACAGGGGCGACAACGTCAACG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 145

CTTCAAGGAGTTCCACGCCAACAACATCCGTGACACCTTCCGTATCCTGCTCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAGGAGAGGGACGGCGTCAAGCTGCCAAGCTACAGGGGCGACAACGTCAACG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 228

CTTCAAGGAGTTCCACGCCAACAACATCCGTGACACCTTCCGTATCCTGCTCCAGATGGGCGCCGTGCTCATGTTCGGTGGTCAGGTGCCGGTCGTCAAGgtaccaatac ... tgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAGGAGAGGGACGGCGTCAAGCTGCCAAGCTACAGGGGCGACAACGTCAACG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaccaataccaaccaacacgtcaaattcaccaggcatgccgatttgctagatctgagccgacctgttccgactttgttgtgcacggtagGTGGGGAGGATGGCTGGCCAGTTTGCCAAGCCAAGGTCCGAACCGTTGGAGGAGAGGGACGGCGTCAAGCTGCCAAGCTACAGGGGCGACAACGTCAACG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT