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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gaactttacattttgactgttccattgcctaatgctttaatattaatcgacaaaatacgcctagtccttgtatttgaccccagtctattcctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC

Basic information

species Zea mays
transcript GRMZM2G103345_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G103345_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os09g39462.1 (Oryza sativa), 3'ss of exon 1
gaactttacattttgactgttccattgcctaatgctttaatattaatcgacaaaatacgcctagtc-cttgtatttgaccccagtctattcctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC
 |   ||  || || |   ||   |  | |  |   |  |  ||    |   | | | || | ||  ||||||  | ||  |||||| |  ||   || ||||||||| ||||||||||| |||||||||||||||||||||||||  |  ||||||| ||| ||| |||| ||||| ||||| || ||||||||||||||
tactgttgtatcttcatgattgactacctttctaactacaagttgtaagttgagaaatgcttcgtggcttgtaccttacagcagtct-tatctcattgtagTGCTAAGGATGGAGCAAGACATTTTGAAGTTCATTCATGACCCTACGCAGACAGAGTTTGACTTCCATGGTCTTCCTACTTCTTACCTGCGTCTTGCTGC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149010836|gb|EE042218.2|EE042218
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|149079416|gb|EE168394.2|EE168394
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|149112744|gb|EE295566.2|EE295566
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|91053358|gb|EB163776.1|EB163776
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|211525651|gb|FL218421.1|FL218421
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|211525649|gb|FL218419.1|FL218419
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|18173981|gb|BM349369.1|BM349369
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|148954293|gb|EC899700.2|EC899700
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTAGAAGTTCATTTCATG
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATT-CATG
EST: gi|149059480|gb|EE156965.2|EE156965
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|211117939|gb|FL431800.1|FL431800
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|101387725|gb|EB815877.1|EB815877
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
EST: gi|211525648|gb|FL218418.1|FL218418
EST:     CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTG                         TGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA
genomic: CGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 214

GGCGCGCCTTCGGCTCCCTCAGATGCGCGCGAGGATGTGGTAGCCCAGGTCGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 198

GGCGCGCCTTCGGCTCCCTCAGATGCGCGCGAGGATGTGGTAGCCCAGGTCGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 249

GGCGCGCCTTCGGCTCCCTCAGATGCGCGCGAGGATGTGGTAGCCCAGGTCGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 155

GGCGCGCCTTCGGCTCCCTCAGATGCGCGCGAGGATGTGGTAGCCCAGGTCGATCAGTTCCTACGAGAGGCCCTCGAGAAGCCAAGGGAGAGGCTTTCTGgtaaaattaa ... ctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atcgacaaaatacgcctagtccttgtatttgaccccagtctattcctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC
                                      tctattcctttctgc  CT-rich tract
                      ttgtattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gaactttacattttgactgttccattgcctaatgctttaatattaatcgacaaaatacgcctagtccttgtatttgaccccagtctattcctttctgcagTGCTAAGAATGGAGCAAGAAATTTTGAAGTTCATTCATGACCCTAGTCGTACAGAGTATGAATTCAATGGCCTTCCAACTTCGTATCTGCGTCTTGCTGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - tacattt
- - - - - - - - - - -ccattgc
- - - - - - - - - - - - - - - aatgctt
- - - - - - - - - - - - - - - - - - - - - - - -cgacaaa