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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ataacacactattattcagtgatgtattctgtctacaaaatgtacttacataatctatttcttaccaatagtttgttgataatcgccagctatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG

Basic information

species Zea mays
transcript GRMZM2G098434_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G098434_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os12g38930.1 (Oryza sativa), 3'ss of exon 1
ataacacactattattcagtgatgtattctgtctacaaaatgtacttacataatctatttcttaccaatagtttgttgataat-cgccagctatt--tttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG
      |   ||   ||| | || ||| |    |  ||  ||| | ||  | |  | | | |   ||||   ||| |  |||| |   || | ||  | |||||||||||||| ||||| |||||||| || |||||||||||||| |||||||| ||||||
--gctgcctgatccatca-taatttatacatgttcaaatttgttcatagttgaaatttgtgtggtcaatgacttgctactaattcatgagttgttactatcagATTGTCTAGGCAGACGGTTAGGTCCACGGCTTCTTGGTAGGGGCAACGATGGCTCCATG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149078797|gb|EE167605.2|EE167605
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCCCGCCTTC
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTC
EST: gi|71436946|gb|DR817996.1|DR817996
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
EST: gi|67040219|gb|CO466474.1|CO466474
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGG-AAACGATG
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
EST: gi|148947655|gb|EC893135.2|EC893135
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
EST: gi|148966012|gb|EE016944.2|EE016944
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTTCT-GGTAGGGGAAACGAT
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTT-CTTGGTAGGGGAAACGAT
EST: gi|76286619|gb|DV026187.1|DV026187
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
EST: gi|148956132|gb|EC901900.2|EC901900
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
EST: gi|148995862|gb|EE035098.2|EE035098
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCCTTCT-GGTAGGGGAAACGAT
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCC-TTCTTGGTAGGGGAAACGAT
EST: gi|148965944|gb|EE016860.2|EE016860
EST:     ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAG                         ATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG
genomic: ACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 28

GTCTTCCACACCGTCCTCATCCTCGCCTTCCTCCTGCCCTTCGTCTTCATACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG


Block sizes: 45 (upstream exon), 43 (downstream exon)
Score: 30

GTCTTCCACACCGTCCTCATCCTCGCCTTCCTCCTGCCCTTCGTCTTCATACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 47

GTCTTCCACACCGTCCTCATCCTCGCCTTCCTCCTGCCCTTCGTCTTCATACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 62

GTCTTCCACACCGTCCTCATCCTCGCCTTCCTCCTGCCCTTCGTCTTCATACTCACCGCCATCATGACGCTAGAGGGCTTCAACAAGTGCTCCTCACTAGgtacatacgt ... tatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttacataatctatttcttaccaatagtttgttgataatcgccagctatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG
                                            ctatttttc  CT-rich tract
     taatctatttctta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ataacacactattattcagtgatgtattctgtctacaaaatgtacttacataatctatttcttaccaatagtttgttgataatcgccagctatttttcagATTGTCTAGGAAGACGTTTAGGTCCGCGCCTTCTTGGTAGGGGAAACGATGGTTCCATG

- aacacac
- - - - - - - - - -tgatgta
- - - - - - - - - - - - - - - - ctacaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgttgat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -cgccagc