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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtcgctgcccgatccgatcccactcgcgtttagtctagtttgttccgttgcctctttttttttttgactcgatgcgtttatgtactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG

Basic information

species Zea mays
transcript GRMZM2G086489_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G086489_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os04g55960.1 (Oryza sativa), 3'ss of exon 1
-------------------------------------gtcgctgcccgatccgatcccactcgcgtttagtctagtttgttccgttgcctctttttttttttgactcgatgcg-tttatgtactcat----cagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG
                                       | || |    | |  |      |||| ||   |   |||  ||        |||  |   || || ||||| || ||| | |     |    ||| | ||||| ||||||||||||||||||||||||||||| ||||||||||| |||||||||
gtcgctgttcgatccgatccgcctccgatccaaaagttcccctcctttgttcttttttttgcgcgattttgcctttttaatcttcgtgtacttactcgattcgattcgattcggtttgtattgctttgctgcagGCGATGGCAGAGGAGGCGAGGGCGCGGTACGAGAAGGCGGTGTTCAAAGTTGTGGATCTG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148941319|gb|EC886225.2|EC886225
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
EST: gi|22472411|gb|BU036891.1|BU036891
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
EST: gi|148961269|gb|EE011991.2|EE011991
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCCGTGTTCAAAGT
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCC-GTGTTCAAAGT
EST: gi|26558402|gb|CA830637.1|CA830637
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
EST: gi|211095653|gb|FK967022.1|FK967022
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
EST: gi|211098118|gb|FK967027.1|FK967027
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
EST: gi|148940766|gb|EC885587.2|EC885587
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
EST: gi|148970820|gb|EE021584.2|EE021584
EST:     TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAG                         TCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG
genomic: TCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 40 (upstream exon), 49 (downstream exon)
Score: 125

GCCGGTCAAGGTGGACGCCGACGCCGACGTTGATGACGGCCGGAAGCGCGTCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 201

GCCGGTCAAGGTGGACGCCGACGCCGACGTTGATGACGGCCGGAAGCGCGTCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 171

GCCGGTCAAGGTGGACGCCGACGCCGACGTTGATGACGGCCGGAAGCGCGTCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 212

GCCGGTCAAGGTGGACGCCGACGCCGACGTTGATGACGGCCGGAAGCGCGTCACCATCTTCTTCGGCACACAGACTGGCACCGCCGAGGGGTTCGCAAAGgtcgctgccc ... tactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtttgttccgttgcctctttttttttttgactcgatgcgtttatgtactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG
                        ttttgac  putative branch site (score: 212)
               tctttttttttttga  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtcgctgcccgatccgatcccactcgcgtttagtctagtttgttccgttgcctctttttttttttgactcgatgcgtttatgtactcatcagTCCATGGCGGAGGAGGCGAGGGCGCGGTACGAGAAGGCCGTGTTCAAAGTGGTGGATCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGTGGA