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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...cctatagctgttttggtattatgttgataataagaattgaaacagatatgattgttcatactatttctgatacaggctgctgtatattttattcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA

Basic information

species Zea mays
transcript GRMZM2G079668_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G079668_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os05g23740.1 (Oryza sativa), 3'ss of exon 1
cctatagc-tgttttggtattatgttgataataagaat-tgaaacagatatgattgttcatactatttctgatacaggctgctgtatattttat-tcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA
   ||||| |||    | |  |||||     || |  |   ||| |  | | || ||||| | | ||| | || |  | || ||   ||||||| |  |  ||||||| ||||||||||||||||||||||||||||| ||||| || || |||||||||||||||||||||||||| || || ||||| || ||||||||||
-aaatagcatgtgcatgcaa-atgttttatgtatgcctgcaaaataagttttatggttcaaattgtttttaatccgag-tgttgatcattttatgttctccagGTGCTTAGAAAGCTGGTGGATGATGCATCAAAGTTCTTGAACGATAAAGTCACAAAGGCAGTGATCACAGTCCCAGCATATTTCAATGACTCACAAAGGA

upper sequence: GRMZM2G079668_T01 (Zea mays), 3'ss of exon 1
lower sequence: PP1S153_153V6.1 (Physcomitrella patens), 3'ss of exon 1
-------------------------------------------------------------cctatagctgttttggtattatgttgataataagaattgaaacagatatgattgttcatactatttctgat-acaggctgctgtatattttattcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA
                                                              | |  |  |  | |   |   |     |||  |  ||               |           ||||||    || |  ||   || || |   || ||||| |||| ||  |||||||||| || || ||||||||||| ||||||||| | ||||| ||| | || ||||| || |||||||||||  | || ||||
gtagggcgtgtgtggtggagattgcggaggggcgggtgggcgtgaattggtattgggattggcgaggggggcgtggaggtggcgggaggaatgggtgtttccgggtgaggaggaggaagaggagggtctgattgtggggttttgggaatgttgtgagtgcagGTGTTGAGGAAATTGGTGGATGACGCTTCGAAGTTTTTGAACGACAAGGTCGCGAAGGCCGTGGTGACGGTCCCGGCGTACTTCAACGACGCCCAGAGGA

upper sequence: GRMZM2G079668_T01 (Zea mays), 3'ss of exon 1
lower sequence: EFJ09963 (Selaginella moellendorffii), 3'ss of exon 2
cctatagctgttttggtattatgttgataata-agaattgaaacagatatgattgttcatactatttctgatacaggctgctgtatattttattcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA
                      ||   ||||  |   ||      | | ||  |||| |   |   |  ||||| |  |  | | | | || || | | ||||  ||||||||||||| || || || || ||||||||||| ||||| || |  ||||| || ||||| || |||||||||||||| ||||| || || |
----------------------gtgagtaatctattcttctctttggtctg-ctgttaacgttgagt--gatac-gttttttctttctcttgtt-tggcagGTTTTGAGAAAGCTGGTCGACGACGCTTCCAAGTTTTTGAACGACAAAGTGAACAAGGCTGTCATCACTGTTCCTGCTTACTTCAATGATTCCCAGAGAA

upper sequence: GRMZM2G079668_T01 (Zea mays), 3'ss of exon 1
lower sequence: EFJ23703 (Selaginella moellendorffii), 3'ss of exon 1
cctatagctgttttggtattatgttgataataagaattgaaacagatatgattgttcatactatt-tctgatacaggctgctgtatattttattcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA
                         |  | |||    |    |   |  |  || |  ||   || | |||||| |  || | | | | || || | | ||||  ||||||||||||| || || || || ||||||||||| ||||| || |  ||||| || ||||| || |||||||||||||| ||||| || || |
-------------------------gtgagtaaactgttcttctctttggtctgctgttaacgttgtgtgatac-gtttgttctttctcttgtt-tggcagGTTTTGAGAAAGCTGGTCGACGACGCTTCCAAGTTTTTGAACGACAAAGTGAACAAGGCTGTCATCACTGTTCCTGCTTACTTCAATGATTCCCAGAGAA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149079084|gb|EE167988.2|EE167988
EST:     ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAG                         GTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
genomic: ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
EST: gi|149109009|gb|EE293094.2|EE293094
EST:     ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAG                         GTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
genomic: ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
EST: gi|71446645|gb|DR827695.1|DR827695
EST:     ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAG                         GTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
genomic: ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
EST: gi|18965171|gb|BM661431.1|BM661431
EST:     ATTGCCCGGCCATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAG                         GTGCTGAGAAAGCTGGTGGATGATGCATCAAAG
genomic: ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAG
EST: gi|87153250|gb|DY398039.1|DY398039
EST:     CAGCTGAGGAGATCTCCGCACAG                         GTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
genomic: CAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
EST: gi|71761337|gb|DR959274.1|DR959274
EST:     ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAG                         GTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC
genomic: ATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 67

GCAGGTCTCGTATCTCGTCATCAGGGACGACAACGGCAACGTCAAGCTCGATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 216

GCAGGTCTCGTATCTCGTCATCAGGGACGACAACGGCAACGTCAAGCTCGATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA


Block sizes: 44 (upstream exon), 46 (downstream exon)
Score: 17

GCAGGTCTCGTATCTCGTCATCAGGGACGACAACGGCAACGTCAAGCTCGATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA


Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 152

GCAGGTCTCGTATCTCGTCATCAGGGACGACAACGGCAACGTCAAGCTCGATTGCCCGGCGATTGGCAAGCAGTTCGCAGCTGAGGAGATCTCCGCACAGgtttaacatt ... attcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atatgattgttcatactatttctgatacaggctgctgtatattttattcttgtagGTGCTGAGAAAGCTGGTGGATGATGCATCAAAGTTTTTGAATGACAAGGTCACAAAGGCAGTGATCACAGTCCCTGCTTACTTCAACGATTCACAAAGGA
                   ttctgat  putative branch site (score: 152)
                                         ttttattctt  putative PPT
                                     tatattttattcttgt  TA-rich tract