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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgatgcatcctcggccgcttcgattctccccaccccaaccccgatccgattcggctaggttttgttgatcatctgacgtgtggcggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC

Basic information

species Zea mays
transcript GRMZM2G078933_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G078933_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os03g18180.1 (Oryza sativa), 3'ss of exon 1
gtgatgcatcctcggccgcttcgattct-ccccaccccaaccccgatccgattcggctaggttttgttga-tcatctgacgtgtggcggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC
|| |  |  |  |  |  | ||||| |  ||||   || | | |   | | || |    |    ||  || || | |    | |||   |   |||||||| ||||||| ||||| ||||||||||||| |||||| ||||| ||  | ||||| ||||| ||||||||||| |||||||||||||||| |||||||
gtaagccgcctccccctccctcgatccagccccgatccgatctcccccggctttgatctgagcctgacgactcgttt--tttttggatttcccgcagGAAGTCGAAGCTGTACCGCTTCGACAAGGAGGGGAACCAATGGAAGGAGAGAGGCACCGGCACGGTGAAGCTGCTGAAGCACAAGGAGACCGGCAAGGTC

upper sequence: GRMZM2G078933_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA01G29410.1 (Glycine max), 3'ss of exon 1
gtgatgcatcctcggccgcttcgattctccccaccccaaccccgatccgattcggctaggttttgttgatcatctgacgtgtggcggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC
        | |    || |    |  |||    | |    |  ||   ||||  |   | |||   ||   ||    | ||| |  |||||  |||||||||||||| || ||||| |||||||||| |||||||||||| || ||||||   || |  || |||||||| || || ||||  ||||  ||||| 
-------gtacgaaaccacacacacactct---ctctgttcttgaattgattttgt--gttttgaatgtgaattgagcttgttgttgttat--agGAAGGCGAAGCTGTATCGGTTTGACAAGGAGGGGAACCAGTGGAAGGAGCGTGGCGGCGGGAACGTTAAGCTGCTGAAACATAAGGTCACCGGGAAGGTG

upper sequence: GRMZM2G078933_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA10G36500.1 (Glycine max), 3'ss of exon 1
gtgatgcatcctcggccgcttcgattctccccaccccaac--cccgatccgattcggctaggttttgttgat-catctgacgtgtgg-----cggttat-gcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC
|| | || || |        ||| || || ||       |  ||||||   | |    |     |  ||| | |   ||| |  ||      || | || |||| ||  ||||||| ||||||||||||||||| | |||||||||||| || |||||| | |||||||||||| | || ||||| ||||   ||| |||||| 
gtaacgc-tctttctttctctcgtttttctccttttttgcttcccgattttactgttttgccactgattggtgcggttgatgcttgttttctcgctgatcgcagCAAATCGAAGCTTTACCGGTTCGACAAGGACGGGAACCAGTGGAAGGAGCGTGGCGCCGGCACTGTGAAGTTCCTGAAGCATAAGGCTTCCGGCAAGGTT

upper sequence: GRMZM2G078933_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA20G31070.1 (Glycine max), 3'ss of exon 1
gtgatgcatcctcggccgcttcgatt-ctccccaccccaaccccgatccgattcggctaggttttgttgat-catctgacgtgtg------gcggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC
|| |  |    ||   | ||    || |||| |        | ||||   | |    |     |  | | | |   ||| |  ||      |||| |  |||| ||  ||||||| ||||||||||||||||| | |||||||||||| || || ||| | ||||| |||||| | || ||||| ||||  || | |||||| 
gtaactctctttccttctctctattttctcctcttttgcttctcgattttactgttttgccactgatcggtgcggttgatgcttgttcctcgcggat-cgcagCAAATCGAAGCTTTACCGGTTCGACAAGGATGGGAACCAGTGGAAGGAGCGAGGCGCGGGCACCGTGAAGTTCCTGAAGCATAAGGCTACGGGCAAGGTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60354018|gb|DN220991.1|DN220991
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|23199554|gb|BU582119.1|BU582119
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270789|gb|FL137290.1|FL137290
EST:     GAGGTCGCCGTTACCA-CGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270777|gb|FL137278.1|FL137278
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270790|gb|FL137291.1|FL137291
EST:     GAGGTCGCCGTTACCAC-GGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|5268837|gb|AI770801.1|AI770801
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211524509|gb|FK953544.1|FK953544
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|29167985|gb|CB411245.1|CB411245
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270785|gb|FL137286.1|FL137286
EST:     GAGGTCGCCGTTACCA-CGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|60349763|gb|DN216736.1|DN216736
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270780|gb|FL137281.1|FL137281
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|31440969|gb|CD510400.1|CD510400
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|29544477|gb|CB604857.1|CB604857
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|30306517|gb|CD001190.1|CD001190
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|22472860|gb|BU037340.1|BU037340
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|31349368|gb|CD433725.1|CD433725
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270788|gb|FL137289.1|FL137289
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270784|gb|FL137285.1|FL137285
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|149110451|gb|EE190423.2|EE190423
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|5555559|gb|AI881510.1|AI881510
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270786|gb|FL137287.1|FL137287
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|29130800|gb|CB381504.1|CB381504
EST:     GAGG-CGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|22935712|gb|BU571987.1|BU571987
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|31910180|gb|CD651295.1|CD651295
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
EST: gi|211270779|gb|FL137280.1|FL137280
EST:     GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACAT                         GAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG
genomic: GAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 88

GCGAGGATGAGGACACCGGCGCTCAGGTCGCGCCCATCGTGAAGCTGGAGGAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 174

GCGAGGATGAGGACACCGGCGCTCAGGTCGCGCCCATCGTGAAGCTGGAGGAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC


Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 115

GCGAGGATGAGGACACCGGCGCTCAGGTCGCGCCCATCGTGAAGCTGGAGGAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC


Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 103

GCGAGGATGAGGACACCGGCGCTCAGGTCGCGCCCATCGTGAAGCTGGAGGAGGTCGCCGTTACCACCGGAGAGGAGGACGAGGACGTACTTGTCGACATgtgatgcatc ... ggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cccgatccgattcggctaggttttgttgatcatctgacgtgtggcggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC
                               atctgac  putative branch site (score: 103)
                    ttttgtt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgatgcatcctcggccgcttcgattctccccaccccaaccccgatccgattcggctaggttttgttgatcatctgacgtgtggcggttatgcagGAAGGCGAAGCTCTACCGGTTCGACAAGGAGGCGAACCAGTGGAAAGAACGTGGCACAGGCACTGTGAAGCTGCTTAAGCACAAGGAGACCGCCAAGGTC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGAGA