ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtattttatttattatccacctctgcaactggagcttccaggagagataaatttgtttctcctcctgctgccactgtttatctggcccggtgctaaccttttgggattcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA

Basic information

species	Zea mays
transcript	GRMZM2G060800_T02
intron #	1
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G060800_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os11g08300.1 (Oryza sativa), 3'ss of exon 2

gtattttatttattatccacctctgcaactggagcttccaggagagataaatttgtttctcctcctgctgccactgtttatctggcccggtgctaaccttttgggattcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA
   |  || | | |||   | | ||||  |  ||  ||||| |   ||  |||      |  | ||  ||    |      ||   |   |||||||| |     ||   || |||||||||||| ||| | ||||||||||| ||  | || ||||| ||| | ||||| || || ||||| ||  |||||||||| ||||||||||||||| 
--gtagtact-actattt-cttttgcatgttcagtctccagtact-atccattaaaagtttattcttttgttgatcagatgctaatc--atgctaaccctcataaatg-atgatcagGTACCTGCCGCCCTCACTACATTCCCATCAACTGCACAAATTGTGTCAGAGCCTCTTGGTGTCGTCCTAGTCATCTCAGCATGGAACTACCCTTTCT

upper sequence: GRMZM2G060800_T02 (Zea mays), 3'ss of exon 1
lower sequence: EFJ32729 (Selaginella moellendorffii), 3'ss of exon 2

gtattttatttattatccacctctgcaactggagcttccaggagagataaatttgtttctcctcctgctgccactgtttatctggcccggtgctaaccttttgggattcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA
                                                                  | |   |  | |    |   |    | |   ||||||    | |    |||||  || |  | || |  |  || || || ||||   ||||||| || |||||||||||||| | |||| | ||||| |||||||||| ||||||  
---------------------------------------------------------------gtagttttgatcgatcgattttgcaaaagatcgtcttttgctgctgg----cagGTCTCTACATCGATGATCAGCTTTCCTTCATCCGGAGAAATCGTCCCAGAGCCCCTCGGCGTTGCGCTCGTTATCTCTGCCTGGAACTTCCCTTTTC

upper sequence: GRMZM2G060800_T02 (Zea mays), 3'ss of exon 1
lower sequence: EFJ20694 (Selaginella moellendorffii), 3'ss of exon 2

gtattttatttattatccacctctgcaactggagcttccaggagagataaatttgtttctcctcctgctgccactgtttatctggcccggtgctaaccttttgggattcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA
                                                                  | |   |  | |    |   |    | |   ||||||    | |    |||||  |  |  | || |  |  || || || ||||   ||||||| || |||||||||||||| | |||| | ||||| |||||||||| ||||||| 
---------------------------------------------------------------gtagttttgatcgatcgattttgcaaaagatcgtcttttgctgctgg----cagGTCTCGACATCGATGATCAGCTTTCCTTCATCCGGAGAAATCGTCCCAGAGCCCCTCGGCGTTGCGCTCGTTATCTCTGCCTGGAACTTCCCTTTCC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|58029607|gb|CX725034.1|CX725034

EST:     GGATGAAGCCCGAGAAG                         GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAG
genomic: GGATGAAGCCCGAGAAGgtattttatt ... tcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAG

EST: gi|78111538|gb|DV529935.1|DV529935

EST:     CGTGCAAGTTTGCGCTCAAGGGGCTCAAGAACTGGATGAAGCCCGAGAAG                         GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAG
genomic: CGTGCAAGTTTGCGCTCAAGGGGCTCAAGAACTGGATGAAGCCCGAGAAGgtattttatt ... tcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 187

CTGCTTCTCCTGTGTTGTGTTGGTGCCTCAGATATCACTTACAAAGTCGTCGTGCAAGTTTGCGCTCAAGGGGCTCAAGAACTGGATGAAGCCCGAGAAGgtattttatt ... tcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1021

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 982

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 856

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tcctgctgccactgtttatctggcccggtgctaaccttttgggattcgtgctcagGTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA