ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...cataatcgaattccttggtattaatgctgctcactatctgtttgtttcatgtatatgttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT

Basic information

species	Zea mays
transcript	GRMZM2G060284_T01
intron #	1
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G060284_T02 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os05g43450.1 (Oryza sativa), 3'ss of exon 1

cataat--cgaattccttggtattaatgctgctcactatctgtttgtttcatgtatatgttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT
 ||  |  |   |||  || || |       |||| | ||| |||| ||   ||   | |  |  || |   |||| | ||||  ||||||||  ||| ||||||||||||||  |||||||||||||||| || |||||||| ||||| ||||| |||||||||| ||| ||||| || || |||||||||||||| || |
-attgttccccgttctatgttagtttcatcactcatt-tctttttgcttgtggtgcctatagttacatcattcattataacatggtgctattggcatggcagTTCAAGATGGAGATGGCTCAAACTAACACTGGCAATACACCAAAGAGTTACTCCTTGAATATGTCCAAGGATTTTGTACCAATGTGTGTTTTCTCTGAGT

upper sequence: GRMZM2G060284_T02 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA04G03030.1 (Glycine max), 3'ss of exon 1

cataatcgaatt-ccttggtattaatgc-tgctcactatc--tgtttgtttcatgtatatgttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT
  |  | |||||  |   ||||  ||   ||||    ||   | |||||| | |||||   | |  |    |  ||||  |  || ||   ||    ||| ||||| |  |||||  |||||    |  |  | | |||||  || || |  || || ||||||||||| ||||| || |||||||||||||| ||||||||  
-cttctagaattgtcacagtatagatatgtgcttctgattaatatttgttgcttgtattgatgtaagagagt--cattgatttataat-tcataattatgtcagTTTACAATGGAGATGGCTGGCTCCGAAGCTGTGAATATGCCAAAAACTTATTCATTGAATATGTTTAAAGACTTTGTTCCTATGTGTGTCTTCTCCGAGA

upper sequence: GRMZM2G060284_T02 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA06G03070.1 (Glycine max), 3'ss of exon 1

--cataatcgaattccttggtat-taatgctgctcactatctgtttgtttcatgtatatgttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT
   ||  || |     | | ||  |  | ||| | | ||| ||||  ||      || |    | |    |   |||  ||  ||||   ||    ||| ||||| |  |||||  ||||    ||  |  | | |||||   | || |  || || ||||| ||||| ||||| ||||||||||||||||| ||||| ||  
agaattgtcaacaatatagataagtgcttctgattaatatttgttgattgtgctgatgtaagatagtcattg--atttatagctcat-tcataattatgccagTTTACAATGGAGATGGCAGGCACAGAAGCTGTGAATATGTCCAAAACTTATTCATTGAACATGTTTAAAGACTTCGTTCCTATGTGTGTCTTCTCTGAGA

upper sequence: GRMZM2G060284_T02 (Zea mays), 3'ss of exon 1
lower sequence: Vv18s0001g13370.t01 (Vitis vinifera), 3'ss of exon 1

--cataatcgaattccttggtattaatgctgctcactatctgtttgtttcatgtatat-gttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT
   ||  | | |  | ||| |  |  | ||  |   |    | || |||  |||  || || ||| |||||  |   |||||||  | || |    ||  |||||||  |||||| || ||   |||    |    ||||  || || || ||||| |||||||||||||||||||| ||||||||||||||||| || ||  
agaatttttgcacccattgttggttgttctaatatgttagagattatttgttgtgaattgtcttgccaccttgc---cttacatgcttctttaatgatagcagTTCACAATGGAAATGACTGGCACTGGGGCTCCTAATATGCCAAAAAGTTACTCCTTGAATATGTTCAAAGATTTTGTTCCTATGTGTGTTTTTTCTGAGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|116816147|gb|EC858853.1|EC858853

EST:     CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

EST: gi|28187707|gb|CB179317.1|CB179317

EST:     CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

EST: gi|211224212|gb|FK968311.1|FK968311

EST:     GTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: GTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

EST: gi|149085565|gb|EE175077.2|EE175077

EST:     CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

EST: gi|76937460|gb|DV175147.1|DV175147

EST:     CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

EST: gi|211224215|gb|FK968314.1|FK968314

EST:     CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: CCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

EST: gi|211224214|gb|FK968313.1|FK968313

EST:     GACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAG                         TTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC
genomic: GACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 36 (upstream exon), 49 (downstream exon)
Score: 49

TTCCTCCGATGCTGCCAACGCCAACCCCGTCGTTGCCAAGGTCGTCCTTTCCCTTGACCTGTTGCGCCAAGAAGAACGCCCGGAAGAGCCTACGCTCCAGgcaagtaccc ... gcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 111

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 128

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 95

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttcatgtatatgttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT

                           ttcttac  putative branch site (score: 95)
      tatatgtttt  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cataatcgaattccttggtattaatgctgctcactatctgtttgtttcatgtatatgttttggcacctaccattcttacatcatgctattgcaatgacagTTCAAGATGGAATTGGCTCAAACTAACACCGGGAATACACCTAAGAGCTACTCTTTGAATATGTTCAAAGATTTCGTTCCTATGTGTGTTTTCTCCGAAT

- - - - - - - - - - - - -gctgctc
- - - - - - - - - - - - - - - - - - - - - - - - - - - -tgttttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - gcaccta
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ccattct
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -catgcta