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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaaccctgaaaatatgcttacaaaatgggaaaaaaaagaaaccgaaaactaaagaactttatgaaatggttggagttctatgattatcttcacgtgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG

Basic information

species Vitis vinifera
transcript Vv14s0060g00820.t01
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: Vv14s0060g00820.t01 (Vitis vinifera), 3'ss of exon 8
lower sequence: LOC_Os12g23180.1 (Oryza sativa), 3'ss of exon 8
gtaaccctgaaaatatgcttacaaaatgggaaaaaaaagaaaccgaaaactaa---agaactttatgaaatggttggagttctatgattatcttcacgtgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG
               | | |   |     |||     ||   || | || | |    | |||||  |  | | |||     | ||||  |||||   |||||||   | ||  || |  ||| ||  || || || ||||| ||||| || ||||||||||||||||| |||||||| || || ||||| || ||||| ||| | ||||||
--------------gtacgtggcatcaacgaagctgtagcggccaagaaatgacatgggactttccggcaggattg-----ccatga--atcttggtgtgtttgaaatttg-atcatgcagGCTGGAGGATTCCCCGAGCCGGAGATCGTCCACTACAACCCCAAGGACTTCGATTTCGGGAAGAAGAAGGCCTTCCCCTTCAGAGACCAG

upper sequence: Vv14s0060g00820.t01 (Vitis vinifera), 3'ss of exon 8
lower sequence: GLYMA03G38930.1 (Glycine max), 3'ss of exon 8
----gtaaccctgaaaatatgct-tacaaaatggg-aaaaaaaagaaaccgaaaactaaagaactttatgaaatggttggagttc-tatgattatcttcacg--tgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG
    || |  |     || |  | |  ||  ||   || | |||| |   | ||| |     | | | | ||    ||  | ||  ||    |||  | |    ||||| ||||| |  | ||  ||| ||  ||||| || ||||| |||||  ||||||||||||| || || || |||||||| ||||||||| |||| |||||||||||||||
gtaagtcattcatgccatgtcatatttaaggtgaccaatagaaagcagaaggaaaatgccttaatatgtcaagcaattaaatttgatacatgtattgtaatttttgtttattttg-gcattttccagGCTGGTGGGTTCCCAGAGCCAGAGATCATTCACTACAACCCTAAAGATTTTGATTTTGGGAAAAAGAAATCATTTCCATTCCGTGACCAG

upper sequence: Vv14s0060g00820.t01 (Vitis vinifera), 3'ss of exon 8
lower sequence: GLYMA19G41540.2 (Glycine max), 3'ss of exon 8
------------------gtaaccctgaaaat-atgcttacaaaatgggaaaaaaaagaaaccgaaaactaaagaactttatgaaatggttggagttctatgattatcttcacgtgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG
                  || |   | ||  | |    || | |   |    |||| |       ||   ||| || |  ||   |    || | || ||  ||| |  |    ||||||||||| |  | ||  ||| ||  ||||| || ||||| |||||  ||||||||||||| || || || || ||||| ||||||||| |||| |||||||||||||||
gtaagtcattcttgccttgtcatatttaaggtgaccaatagagagcagtgggaaaatgccttaataagtgaaacaattaaatttgagacatgta-ttttagtattgtaatttt-tgtttgttttg-gcattttccagGCTGGTGGGTTCCCAGAGCCAGAGATCATTCACTACAACCCTAAAGATTTTGACTTTGGGAAAAAGAAATCATTTCCATTCCGTGACCAG

upper sequence: Vv14s0060g00820.t01 (Vitis vinifera), 3'ss of exon 8
lower sequence: AT1G09340.1 (Arabidopsis thaliana), 3'ss of exon 8
-----------------------------------gtaaccctgaaaatatgcttacaaaatgggaaaaaaaagaaaccgaaaactaaagaactttatgaaatggttgga--gttctatgattatcttcacgtgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG
                                     || ||  |||   |    |  | ||                 | ||   | |  |   | |||  |  |||||   ||   ||| ||   | |    | | |||    | |||   |||  |  |||||||| ||||| ||||||||||| |||||||| || |||||||| ||||| || ||||| |||||||| |||||||| |||
gtacattcttttctattggctttattgttgtctcatcaatcc--aaatcgtttcaagtacatcctggtgtggtccgtttgtaatagatatcatactctgagctttttggatcattgcttgagtaaacttattcatgt-tttcaactctctctcagGCCGGTGGGTTTCCGGAGCCAGAGATTGTTCATTACAACCCGAAAGAGTTCGACTTTGGGAAGAAGAAGGCATTCCCTTTCCGTGATCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|110377806|gb|EC942895.1|EC942895
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349817662|gb|FQ407651.1|FQ407651
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCTCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110382725|gb|EC947045.1|EC947045
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351052362|gb|FQ455534.1|FQ455534
EST:     CTGGTGAAAAATACGTCGCTTTTGATGGATTGGCAAGGGCATGTGCAAGG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110417826|gb|EC983913.1|EC983913
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349825173|gb|FQ415720.1|FQ415720
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351052958|gb|FQ453945.1|FQ453945
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCTCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110380012|gb|EC945247.1|EC945247
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|352773974|gb|FQ473106.1|FQ473106
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110363102|gb|EC926672.1|EC926672
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|26259188|gb|CA810251.1|CA810251
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTT-
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349814545|gb|FQ399185.1|FQ399185
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351035171|gb|FQ458528.1|FQ458528
EST:     TGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATT
genomic: TGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAA-Ggtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATT
EST: gi|351012098|gb|FQ429904.1|FQ429904
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCTCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110367551|gb|EC931488.1|EC931488
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110378072|gb|EC943185.1|EC943185
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351057074|gb|FQ450781.1|FQ450781
EST:     CTGGTGAAAAATACGTCGCTNTTGATGGATTGGCAAGGGCATGTGCAAGG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351016507|gb|FQ423934.1|FQ423934
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGGGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|352763325|gb|FQ471637.1|FQ471637
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349823731|gb|FQ415142.1|FQ415142
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCTCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110721160|gb|EE097838.1|EE097838
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349825174|gb|FQ415721.1|FQ415721
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351047890|gb|FQ459110.1|FQ459110
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTTCTGAGCCTGAGATTGGTCACTACAACCCC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCC
EST: gi|26259994|gb|CA811057.1|CA811057
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTT-
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|110373613|gb|EC937864.1|EC937864
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349821496|gb|FQ408055.1|FQ408055
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351039971|gb|FQ465009.1|FQ465009
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|349807769|gb|FQ402160.1|FQ402160
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCTCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351055049|gb|FQ440388.1|FQ440388
EST:     CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAG                         GGTGCTGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCTCAAGGAGTTC
genomic: CTGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATGTGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTC
EST: gi|351016399|gb|FQ423826.1|FQ423826
EST:     TGGTGAAAA-TACGTCACTTTTGATGGATTGGCAAGGGCATGGTGTAAAG                         GGTGCTGGGTTTCCTGA
genomic: TGGTGAAAAATACGTCACTTTTGATGGATTGGCAAGGGCATG-TGCAAAGgtaaccctga ... tgtcttatagGGTGCCGGGTTTCCTGA


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gaaatggttggagttctatgattatcttcacgtgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG
                tatgattat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaaccctgaaaatatgcttacaaaatgggaaaaaaaagaaaccgaaaactaaagaactttatgaaatggttggagttctatgattatcttcacgtgtttgttttgtgtgtcttatagGGTGCCGGGTTTCCTGAGCCTGAGATTGTTCACTACAACCCCAAGGAGTTCGATTTTGGCAAAAAGAAAGCATTCCCATTCCGTGACCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA