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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgccactctatcatcagaaacagcatcttttcctctttccccaaaactgacactaaagtgtaatatcatggttcttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA

Basic information

species Vitis vinifera
transcript Vv12s0057g01200.t01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: Vv12s0057g01200.t01 (Vitis vinifera), 3'ss of exon 4
lower sequence: LOC_Os09g07830.1 (Oryza sativa), 3'ss of exon 4
gtgccactctatcatcagaaacagcatcttttcctctttccccaaaactgacactaaagtgtaatatcatgg--ttcttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
  |  ||  | |||| ||  |     ||| |   |  |   | | || | | | | |  ||    |||||||  || |||| ||  |  |   |||||| |||||| | |||||||||   || |  |||||  ||||||||||||||||  | ||||| |||||||||||||||||||  || |||||| | || ||
--gttacagt-tcatgaggcagcctttctataaattctgttcaagaaattatatttacctg----atcatgggcttgtttttat-ttcctttctgcagCGACAATGTTTGCAGCACAGTCAATTCTATTGGGCATCAATGATATTGTTGTAGCCGGTGGCATGGAAAGCATGTCTAATGCTCCAAAATACATTGCTGA

upper sequence: Vv12s0057g01200.t01 (Vitis vinifera), 3'ss of exon 4
lower sequence: GRMZM5G866758_T02 (Zea mays), 3'ss of exon 4
gtgccactctatcatcagaaacagcatcttttcctctttccccaaaactgacactaaagtgtaat------atcatggttct-tttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
||   | || |   |      | |   ||| |   || | | || |  | |||  |  | |  ||      | |||| |  | |||     |  ||  |||||| || ||| | |||||||||   || || |||||| ||||||||||||||||| ||||||| |||||||||||||| ||||  || || ||| | || ||
gtttgaatcgaatttatctgtctgt-ccttgtgtactctgctcagagttcacagaagtgagagattacctgaccatgctcttgttttcctttcctatatgcagCTACTATGTTTGCAGCACAGTCAATTCAATTGGGTATCAATGATATTGTTGTGGCTGGTGGCATGGAAAGCATGTCCAATGCCCCAAAGTACATTGCTGA

upper sequence: Vv12s0057g01200.t01 (Vitis vinifera), 3'ss of exon 4
lower sequence: GRMZM2G085474_T04 (Zea mays), 3'ss of exon 4
-----------------------gtgccactcta-tcatcagaaacagcatcttttcctctttccccaaaactgacactaaagtgta---atatcatg-gttcttttaatgctggta---attgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
                        ||    | || |              |   ||  | ||| | ||          | | ||||     ||||||  |  |  ||| |    | |     |||||| || ||| |||| ||||||   || ||| ||||| ||||||||||||||||| | ||||| |||||||||||||| ||||  || || ||| | || ||
gtctgaagctaattcatctatccatgtttgtataattgatctgcttgctgttcattgttttttacacatgtgaagtgttcaggtgtgtgtgtatcatctgaccaattactctctgcacactctgcagCTACTATGTTCGCCGCACAGTCAATTCAGCTGGGTATCAATGATATTGTTGTGGCAGGTGGCATGGAAAGCATGTCCAATGCCCCAAAGTACATTGCTGA

upper sequence: Vv12s0057g01200.t01 (Vitis vinifera), 3'ss of exon 4
lower sequence: GRMZM5G830250_T01 (Zea mays), 3'ss of exon 2
gtgccactctatcatcagaaacagcatcttttcctctttccccaaaactgaca----ctaaagtgtaatatcatggtt---cttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
                               | ||||   | ||   |  | |      | | | |   |||||| |    |||||  | |   ||  |||||| || ||| | |||||||||   || ||  ||||| ||||||||||||||||| | ||||| |||||||||||||| ||||  || || ||| | || ||
-nnnnnnnnnnnnnnnnnnnnnnnnnnnnaatactctgctcacaggtcgcagaagtgacagattatctgatcatgctcaggcttttctttcctata--tgcagCTACTATGTTTGCAGCACAGTCAATTCAACTGGGTATCAATGATATTGTTGTGGCAGGTGGCATGGAAAGCATGTCCAATGCCCCAAAGTACATTGCTGA

upper sequence: Vv12s0057g01200.t01 (Vitis vinifera), 3'ss of exon 4
lower sequence: GLYMA02G47860.2 (Glycine max), 3'ss of exon 4
gtgccactctatcatcaga--aacagcatcttttcctctttccccaaaactgacactaaagtgtaatatcatggttcttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
  |    | ||| || | |  ||    || | || || | |           | | || ||   ||    ||    |||    | ||||| ||||||||  ||||||| || ||||||||||| || ||  |  | |||||| |||||||| |||||||||||||||||||||||||||| || || ||||| || ||
--gtttgtttattataaaattaattctatttgtttctttgtattttgtttaaaaaatataggaaaaggatattaaactt---gttctggttattgcagCTGCAATGCTTGCTGCACAGAGTATTCAATTAAGCATAAATGATGTTGTTGTGGCTGGTGGTATGGAAAGCATGTCTAATGTACCCAAGTACCTGGCTGA

upper sequence: Vv12s0057g01200.t01 (Vitis vinifera), 3'ss of exon 4
lower sequence: GLYMA14G00760.1 (Glycine max), 3'ss of exon 4
gtgccactctatcatcagaaacagcatcttttcctctttccccaaaactgacactaaagtgtaatatcatggttcttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
|||    | ||  |  |  ||    || | || || | |      |  | | | || ||   ||    ||    |||    | ||||| ||||||||  ||||||| || ||||||||||| || || ||    |||||| |||||||| |||||||||||||||||||||||||||| || || ||||| || ||
gtgtgtttataataaaaacaattctatttgtttctttgtatttttatttaaaaatatagaaaaaggatattaacctt---gttctggttattgcagCTGCAATGCTTGCTGCACAGAGTATTCAATTAGGCACAAATGATGTTGTTGTGGCTGGTGGTATGGAAAGCATGTCTAATGTACCCAAGTACCTGGCTGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|110398909|gb|EC965389.1|EC965389
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110699364|gb|EE076300.1|EE076300
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110403296|gb|EC964774.1|EC964774
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|71859096|gb|DT008151.1|DT008151
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110701385|gb|EE078893.1|EE078893
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|161716985|gb|FC066530.1|FC066530
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|161717757|gb|FC064725.1|FC064725
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|71866622|gb|DT015677.1|DT015677
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|161712803|gb|FC062326.1|FC062326
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110398035|gb|EC964108.1|EC964108
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|32269649|gb|CD718808.1|CD718808
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|30254555|gb|CB971300.1|CB971300
EST:     GTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: GTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|71875811|gb|DT024866.1|DT024866
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110723077|gb|EE099979.1|EE099979
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110686757|gb|EE064757.1|EE064757
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|161720962|gb|FC070684.1|FC070684
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110698167|gb|EE075418.1|EE075418
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110685325|gb|EE063616.1|EE063616
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110723249|gb|EE100343.1|EE100343
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|71855093|gb|DT004148.1|DT004148
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110371396|gb|EC935385.1|EC935385
EST:     CTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: CTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|71859317|gb|DT008372.1|DT008372
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|34543095|gb|CF511327.1|CF511327
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|77587675|gb|DV223012.1|DV223012
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|30299187|gb|CB975981.1|CB975981
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110702702|gb|EE078902.1|EE078902
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|110364569|gb|EC927918.1|EC927918
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
EST: gi|77586011|gb|DV222101.1|DV222101
EST:     TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAG                         CAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG
genomic: TTCAGTGGTCTGTACCACCATCAACAAAGTTTGTGCATCAGGAATGAAAGgtgccactct ... gtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTG


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ccaaaactgacactaaagtgtaatatcatggttcttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA
                               ttcttttaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgccactctatcatcagaaacagcatcttttcctctttccccaaaactgacactaaagtgtaatatcatggttcttttaatgctggtaattgcagCAACAATGCTCGCAGCACAGAGTATCCAGTTGGGTGTCAATGATATTGTTGTGTCTGGTGGTATGGAAAGCATGTCTAATGTGCCTAAATACCTAGCAGA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG