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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG

Basic information

species Vitis vinifera
transcript Vv12s0057g01480.t01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: LOC_Os09g12660.2 (Oryza sativa), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                              ||||| |||  |     | | |  |  | | |    || |   |   |  || | ||| |  |   |  ||  |  | |      | |    ||  |||| || |||||||| || || ||||||  |||||| || || ||| |||||||||||||||||||||| || |||||  | ||||||||||||||| |  
-----------------------------gtaattgatgagtc---atccgttgagaccata----tgcaacct--gatctgttgaagcatacatttcagtgattgtctgcatgttttgatcttgcagGTTGACACTACCATACTTGGCCTTGATACTGAGAGGGCAAAGGAATTGCCTTACATTGCTAGTATGGGAATCTATGTTTTTAGCAAAGATGTGATGCTTA

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: LOC_Os08g25734.2 (Oryza sativa), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
    |||  |  |||  |   | ||| |  ||| || || | |   |||     ||    || || |        | | ||   ||  ||  ||    | ||||  |  ||  | | |    ||  |||| || |||||||| || || ||||| || | ||| || |||||||||||||| |||||||| |||||||| |||||  | || ||| |||| ||| |  
--gtatg-caaatgacgtgc-ctttcttgctaacttgtgatatacagttgtgagatat--cagttattacaccacatacttccatatcaaaact----cattttgttgcct--attttcgtcttgtagGTTGACACTACCATACTTGGCCTTGACGACGTGAGGGCAAAAGAAATGCCTTATATTGCTAGCATGGGTATCTATGTGATTAGTAAAAATGTAATGCTTC

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: GRMZM2G163437_T01 (Zea mays), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaattt---atgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
||| |    |||||   |   | |||    ||| ||   || | |||||   |    |||     |   |  | | ||        || | || ||   | | | |    ||     ||| |   | || ||| || || ||||| || || |||||||| ||||| || || ||||||||||| |||||||| ||||||||||||||  | ||||| ||||| ||| |  
gtacacataatttgcctttt-ctttcaagctaacttgacatacatttgttgacaatcatttagtgaacctaaaatatt----cccctttaaaatgcc--ttcatctcatatttctgtctgattgatgta-gGTTGACACCACCATACTTGGCCTTGATGACGAGAGGGCAAAGGAAATGCCTTATATTGCTAGCATGGGTATATATGTTTTTAGCAAGGATGTAATGCTTC

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: GRMZM2G068506_T03 (Zea mays), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaattta-tgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                       |   ||  | |||| | |||    ||||||| ||| |    || | |  |   || |     | | || ||  || |   |    || |    || |   | || ||| || || ||||| || || |||||||| | ||| || || ||||||||||| |||||||| |||||||| |||||  ||||||||||||| ||| |  
----------------------ttctataggattttaatccttcaaagttcctccatttt----ttctttgttccaaaggagcccttaaaatgc--attcacatcatattgttgtttgattgatgta-gGTTGACACCACCATACTTGGCCTTGATGACGTGAGGGCAAAGGAAATGCCTTATATTGCTAGCATGGGTATCTATGTTTTCAGCAAAGATGTAATGCTTC

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: GRMZM2G106213_T02 (Zea mays), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
       | |  ||  |||||| ||    || |     | | || |||  |   ||| |   |  |  |   |||    |||   |      | | || ||||| |||       | | |||||| |||| || || || ||  |||| ||||||  ||| || || |||||| ||||||| |||||||||||||| || |||||  | ||||||||||||||| |  
-----gtacaaatgtgataaacttt----ttgaccctagttcttatgtctcctgattgttagagctcct---tttgcgattg--cattttttcagttaagttttgctt-----gttttaccttttcagGTAGACACCACTATATTGGGCCTTGATCCTGAAAGGGCCAAAGAACTGCCTTATATTGCTAGTATGGGAATCTATGTTTTTAGCAAAGATGTGATGCTTC

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: GLYMA14G01290.1 (Glycine max), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctat-tctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                    |    | ||| ||  | |  || | ||||  | | | |||  || ||  ||  |  | | ||| |||| || ||                    ||| ||| |||  |||| |||||||| ||  ||||||||||||| |||||||| |||||||||||||| |||||||| |||||||||||||| || |||||| ||||||||||||
------------------gcaagcccatat-aagatgta-ttatatgttgttatttatctggaaattattacctgaacaattgcttaaaaatgcc-------------------acttttcttttctagGTTGATACTACAATTTTGGGTCTTGATGACGAGAGAGCAAAAGAAATGCCTTATATTGCTAGCATGGGTATATATGTTGTTAGCAAAAATGTGATGTTAG

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: GLYMA02G47460.1 (Glycine max), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                                   || ||   | |   | | |||| ||   | || |   | |  |  |  |||     ||   | |         |   || ||   ||    |||| |||||||| ||  ||||||||||||| |||||||| || ||| ||||||| |||||||| |||||||||||||| || |||||| | ||||||||||
----------------------------------gtaagcccatataa-catgtattatatgttgttatttatctggaaattattacctgagcaattgc---------ttaaaaatgccact----agGTTGATACTACAATTTTGGGTCTTGATGACGAGAGAGCAAAGGAATTGCCTTATATTGCTAGCATGGGTATATATGTTGTTAGCAAAAACGTGATGTTAG

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: AT5G48300.1 (Arabidopsis thaliana), 3'ss of exon 2
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                              || | ||  ||||    | | ||| | || || |  ||  |  ||   ||   |||| ||  ||    || |||| ||        | ||||    |||| || || || || || |||||||||||| ||||||| || || ||||||| |||||||||||||||||| ||||| || |||| |||||| ||| |||
-----------------------------gtactataaactttacgctacttctctgcttca-tcatgcggcctt---gtaaattaaaaaatct----cgatttcttt--------gctcct----agGTTGACACAACAATTCTAGGTCTTGATGATCAGAGAGCCAAGGAGATGCCTTTCATTGCTAGTATGGGTATTTATGTTGTAAGCAGAGATGTAATGCTAG

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: PP1S239_45V6.1 (Physcomitrella patens), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                         || | |      ||   |  | | | ||| ||  |  | ||        | |      |||   ||  |     |   |  |||||||  | |     || |||| || || ||  || | ||||| ||||  |  ||||| ||||||||||||||||| || ||||||||||| || ||||||||||| || |  ||| |  
-----------------------gacggaatcggcggtgagctaatttacttctgttgaaggaatt-----acgtgagaaccattagttagtatacagtgacatattttctgatgtttggtgattcagGTGGACACAACTGTCTTAGGTCTAGATGCCGCTAGAGCCAAAGAAATGCCTTACATCGCGAGTATGGGTATTTACGTCGTCAGCAAGGAGGCTATGATCT

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: PP1S76_105V6.1 (Physcomitrella patens), 3'ss of exon 3
---gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgtt----cctctattctacaattttg-tagttttaaagttggttaagaaggctgatacgttttcctttctgaac----tgttcctt--ttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
     | ||| | |      ||||   |    ||  || | | ||   | ||    | | ||       ||  || |  || |     |  ||  |     ||||   || |  | |  |  |      ||  ||  || |||| || || ||  |  ||||| | |||| ||| ||||| ||||||||||| |||||||| ||||||||||| || || || || ||||| |  ||| |  
gtatgaaatgttctccatgttaaaggatta-gttggttgaagattgattattaccacttgtagaggcagatactgctgattgtcggtctatttcggctttttgattttttctaatctacgtgcacggcatttattgattcagGTGGACACGACAGTTTTGGGTTTGGATGCTGAAAGAGCCAAAGAAATGCCCTACATTGCAAGTATGGGTATTTACGTAGTGAGTAAAGAGGCTATGATCT

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: PP1S2_392V6.1 (Physcomitrella patens), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaat-ttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                         |   ||||| |||||      | ||    | ||  ||| |    |   ||  |  |||  ||||    |||||    |  |   |  |   | |      | |||| || || ||  |  ||||| | ||||  ||||| || ||||||||||| |||||||| ||||||||||| || ||||||||||| || |  ||| |  
-------------------------caagttaatcttatggaggattattgagttgtt--acagtgagatctcttctacattg--taagttttctgatgatctacctcatacggtgtatgatgaatcagGTGGACACGACAGTTTTGGGTTTAGATGCCGAGAGGGCCAAAGAAATGCCCTACATTGCAAGTATGGGTATTTACGTCGTCAGCAAGGAGGCTATGATCA

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: EFJ17026 (Selaginella moellendorffii), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttcctttta-agGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                                                             |  ||| | ||  |    ||| ||  |  | ||   |      |  |  ||   || | || ||  |||| || || || || || ||||| |||   || ||||||||||| |||||||||||||| || |||||||| ||||| || || || ||||| |||    
-----------------------------------------------------------gtatatttattgtcgt----ttgtttgcgctgactagaatcgaaacaataatgctatgctgctcttgcagGTGGACACGACAATTCTTGGTCTGGATCCGGAAAGAGCTAAAGAGATGCCTTACATTGCAAGCATGGGTATTTATGTGGTGAGTAAGGATGTCATGAGCA

upper sequence: Vv12s0057g01480.t01 (Vitis vinifera), 3'ss of exon 3
lower sequence: EFJ16157 (Selaginella moellendorffii), 3'ss of exon 3
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaatt-ttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                                                          || | || |||| ||||     | | | |  |     || ||  |     | ||   ||| || |    |||| || || || || || ||||| |||   || ||||||||||| |||||||||||||| || |||||||| ||||| || || || ||||| |||    
---------------------------------------------------------gtatatttattgtcgttt-gttttcgctgactagaatcgaaacaata---atgctatgctgctcttgc--agGTGGACACGACAATTCTTGGTCTGGATCCGGAAAGAGCTAAAGAGATGCCTTACATTGCAAGCATGGGTATTTATGTGGTGAGTAAGGATGTCATGAGCA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33409675|gb|CF215302.1|CF215302
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|71861397|gb|DT010452.1|DT010452
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATC
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATC
EST: gi|161718175|gb|FC065663.1|FC065663
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|110689182|gb|EE065182.1|EE065182
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|27584163|gb|CB006858.1|CB006858
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|30325486|gb|CD008748.1|CD008748
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|30324817|gb|CD008079.1|CD008079
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|71863688|gb|DT012743.1|DT012743
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|30324290|gb|CD007552.1|CD007552
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|33403137|gb|CF208764.1|CF208764
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|30253354|gb|CB970905.1|CB970905
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|110703129|gb|EE079706.1|EE079706
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
EST: gi|27579307|gb|CB002002.1|CB002002
EST:     TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAG                         GTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT
genomic: TAATTGAATTTGCAGAGAAACCAAAAGGAGAGCAATTGAAAGCTATGAAGgtagatgata ... tccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCT


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG
                 ggctgat  putative branch site (score: 4)
                                         ctgttcctttt  putative PPT
                                                ttttaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtagatgatatttgaaataaacattcgtattaatttatgcttttgtgttcctctattctacaattttgtagttttaaagttggttaagaaggctgatacgttttcctttctgaactgttccttttaagGTCGATACTACCATCCTGGGTCTTGATGATGAGAGAGCTAAAGAAATGCCTTACATTGCTAGTATGGGTATATATGTCGTCAGCAAAGATGTGATGTTAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG