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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtttattttgcagtatttttagcagcacatctttaacttctctgtaaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG

Basic information

species Selaginella moellendorffii
transcript EFJ33580
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: EFJ33580 (Selaginella moellendorffii), 3'ss of exon 8
lower sequence: GLYMA15G40110.1 (Glycine max), 3'ss of exon 8
-----------------------gtaagtttattttgcagtatttttagcagcacatctttaact-tctctgtaaat-tgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                        || |||  ||| | |     ||  | |   || |||   |  | | |||| || || |||||||||||||||||||||| ||  | || |||||||| || ||||| ||||||||| | ||||| || |||
gtaacagactcactgaaatggtaatatgttagtttggtacgccgttgggtatatcaacttgggcaatttttgtacatgtgtagGTTGGTGCACAAACTGAGACTGAGCTCAAGGAAAAGAAATTGAGAGTTGAAGATGCTCTTAATGCTACAAAG

upper sequence: EFJ33580 (Selaginella moellendorffii), 3'ss of exon 8
lower sequence: AT1G55490.2 (Arabidopsis thaliana), 3'ss of exon 9
----------------gtaagtttattttgcagtatt---------tttagcagcacatctttaacttctctgtaaattg----aagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                 |||||||  ||||    |||         || ||    |     ||||  ||  | |  ||||    |||||||| |||||||||||||||||| | ||||| |||||||| ||||| ||||||||| | ||||| || |||
gtaaactaaaaactatttaagtttcctttgtctgattaagatgtgtttcagagatattaagttaagctcgttatgtattggttaaagGTTGGAGCACAAACTGAGACAGAACTCAAAGAGAAGAAGTTGAGAGTTGAAGATGCTCTTAATGCAACAAAG

upper sequence: EFJ33580 (Selaginella moellendorffii), 3'ss of exon 8
lower sequence: AT3G13470.1 (Arabidopsis thaliana), 3'ss of exon 9
--------------------------gtaagtttattttgcag-----tatttttagcagcacatctttaacttctctgta--aattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                           |   ||| | |   ||     ||  || ||      |  |  |  || |   |     || |||||||| || ||||||||||||||  | |||||||||||| | ||||||||||||||| | ||||| || |||
gtaaaatagtaatcacatggactttcttggatttgtatgatagaagaataagttaagtgatctaattaaagtttttacatcttggttaaagGTTGGAGCTCAAACTGAGACAGAGCTCAAAGAAAAGAAGCTGAGAGTGGAAGATGCTCTTAATGCTACAAAG

upper sequence: EFJ33580 (Selaginella moellendorffii), 3'ss of exon 8
lower sequence: AT5G56500.2 (Arabidopsis thaliana), 3'ss of exon 9
--------------------------------gtaagtttattttgcagtatttttagcagcacatctttaacttctctgtaaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                                  |||  ||  ||   ||| |   || || | |   | ||| | | | ||   |  ||||||| |||||||||||||||||  | || || ||||| |||||||| ||||||||| | ||||| || |||
gtgagatactaagaaacaaatcacatgatgcaaaaagcgtagcttaaggtaata--agaagaataatctaaacgttt-tatattctttagGTTGGAGCACAAACTGAGACAGAGCTTAAGGAGAAGAAATTAAGAGTTGAAGATGCTCTTAATGCTACAAAG

upper sequence: EFJ33580 (Selaginella moellendorffii), 3'ss of exon 8
lower sequence: Vv06s0004g00240.t01 (Vitis vinifera), 3'ss of exon 9
----------------------------------gtaagtttatt-ttgcagtatttttagcagcacatctttaacttctctgtaaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                                   | | || ||  ||||||  |  |||| | |  ||   | | |  |       ||||||||||| |||||||||||||||||  | |||||||||||  | ||||| ||||| ||| | ||||| ||||||
gtaaattcttagttagtccagttaaaatttataaatgatttcatcattgcag-gtgcttagtatc-tattaattattcattgaacttttgaagGTTGGAGCACAAACTGAGACAGAGCTTAAAGAAAAGAAACTGAGAGTAGAAGACGCTCTCAATGCAACGAAG

upper sequence: EFJ33580 (Selaginella moellendorffii), 3'ss of exon 8
lower sequence: Vv09s0002g03930.t01 (Vitis vinifera), 3'ss of exon 8
-----------------------------------------------gtaagttt-attttgcagtatttttagcagcacatctttaacttctctgta--aattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                                               | || | | |   |   ||  ||||     |||   ||||| || | |||   |  |  |||| || ||||||||||| |||||| | ||||||||||| || |||||||| |||||  | ||||| || |||
gtaatttttttttttcttaaatacaatgtagctgttggttgatagatggaattctgaaagtattgttcttttctacccactattttaatttttgtgttgcacattcagGTCGGAGCACAAACTGAAACAGAACTTAAAGAAAAGAAATTGAGAGTGGAGGATGCCCTTAATGCAACAAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|169077167|gb|FE486093.1|FE486093
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169098700|gb|FE500238.1|FE500238
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAACTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169024290|gb|FE432551.1|FE432551
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAACTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169030365|gb|FE437828.1|FE437828
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAACTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169081608|gb|FE491262.1|FE491262
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169021473|gb|FE428560.1|FE428560
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAACTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169028066|gb|FE435424.1|FE435424
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAACTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
EST: gi|169024240|gb|FE435218.1|FE435218
EST:     AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAG                         GTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA
genomic: AATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 12

GCCACCGAGCAAGACTACGAAAGAGAAAAATTGAACGAAAGAATAGCTAAATTGTCTGGAGGAGTTGCTGTAATCCAGgtaagtttat ... taaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agtttattttgcagtatttttagcagcacatctttaacttctctgtaaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG
                               ctttaac  putative branch site (score: 12)
                                     cttctctgt  CT-rich tract
            agtattttta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtttattttgcagtatttttagcagcacatctttaacttctctgtaaattgaagGTTGGTGCACAAACTGAGACAGAATTAAAAGAAAAGAAGTTAAGAGTGGAAGATGCTTTGAATGCGACGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG