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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA

Basic information

species Selaginella moellendorffii
transcript EFJ35350
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: AC233895.1_FGT001 (Zea mays), 3'ss of exon 5
---------------gtttgta---gctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
                |||| |   |||    ||| |  |     | | |  | ||       | | |  | || |||||| ||| |  | || |||| |||||| ||  |||||||||||||||| |||||||  ||||| ||||||||||| || || |||||||| |||||||| |
gtaagcgattctgttatttgcatatgctgctcctttttttccctgtttgatcacgtctttgttgaatat-tcatggcagCCCAAGTTATTGGATGAGAAACAGACCACCATCTACAAAAGGGCCGTAGACAAGAATTATCACTTGAAGATGAAAGCGTCAAGGTTTATTTTCAGTGAGA

upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: GRMZM2G388234_T01 (Zea mays), 3'ss of exon 2
--------------------gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA------
                    |  |||     |   || |  |  |  | | |  |  |       | | |  | ||  || || ||| |  | || ||||  ||||| ||  ||||||||||||| || ||||||| |||| |  |||||||||| || || | |||||| ||||||||||      
gtaagaaattttgtttttttgcatgtgatgcttcttttttttccatgtatgaccacattttcgttgaatat-tcatgccaacccaagTTATTGGATGAGAAGCAGACCACTATCTACAAAAGGGCTGTAGACAAGAACTATAACCTGAAGATGAAAGCATCAACGTTTATTTTCAGTGAAATCAGCC

upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: GLYMA03G07420.1 (Glycine max), 3'ss of exon 6
---------------------gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
                      |||   | | |||   ||| |   |  ||  |    ||  ||||    |   | | |||||| |||||| | || || |  ||||||||  | || || || || || |||||||||||||| ||||||||||| || || ||||| || ||||||||||
gtaatatagttttattaatatatttaccgatatgatgcatcttcctatcctttgct--tagtaaactaccaatctcttgcagCCTAAGCTTTTGGATGAAAAGCAGACAACTATTTATAAGAGAGCTGTTGACAAGAGCTATCACTTGAAGATGAAAGCATCTAGGTTCATTTTCAGTGAAA

upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: GLYMA01G29420.1 (Glycine max), 3'ss of exon 6
---------------------gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
                      |||   | | |||   ||| |   ||||    |       |||  | ||  |  | |||||| |||||  | || ||||  ||||||||  | || || || || || ||||||||||||||||| |||||||| || || ||||| || ||||||||||
gtaatttggttttattaatatatttactgatatgatgcatcttcctaatcctttgcttagtaaaattccaatctc-ttgcagCCTAAGCTGTTGGATGAGAAGCAGACAACTATTTATAAGAGAGCTGTTGACAAGAGCTATCATTTAAAGATGAAAGCATCTAGGTTCATTTTCAGTGAAA

upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: GLYMA07G18500.1 (Glycine max), 3'ss of exon 6
-------------gtttgtagctttgacttatcgttgaaatctaag---gaagtaag--aaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
              |    |  |||        | |  |   || |   ||  |||   |||     |   | | |||||| |||||  | || ||||  ||||||||  |||| || ||||| || |||||||| ||||| ||||||||||| || || ||||| || ||||||||||
gtagttcaattatatcaacatatttactagtatggtagagcttatgcatgatataaagtaaattttcattctcttgcagCCTAAGCTGTTGGATGAGAAGCAGACAACTATCTATAAGAGGGCTGTTGACAAGAGTTATCACTTGAAGATGAAAGCGTCTAGGTTCATTTTCAGTGAAA

upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: GLYMA16G19310.1 (Glycine max), 3'ss of exon 3
-------------gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatc-----gcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
              |    |  |||        | |  |   || | | |  | |||   ||   | |||     |||||| |||||  | || ||||  ||||||||  |||| || ||||| || |||||||| ||||| ||||||||||| || || ||||| || ||||||||||
gtagttcaattatatcaacatatttactagtatggtagagcttatgcatgatataaagtaaattttcatcctcttgcagCCTAAGCTGTTGGATGAGAAGCAGACAACTATCTATAAGAGGGCTGTTGACAAGAGTTATCACTTGAAGATGAAAGCGTCTAGGTTCATTTTCAGTGAAA

upper sequence: EFJ35350 (Selaginella moellendorffii), 3'ss of exon 5
lower sequence: Vv08s0217g00020.t01 (Vitis vinifera), 3'ss of exon 5
-----------------------------gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
                              ||  |    |||||      ||    | |  |    |||     | || |   || |||||| | ||| || || ||||  ||||||||  | |||||||| || || |||||||||||||||||||||||||| || || || || || |||||||| |
gtaattatgggtctgattttaacccaaatattattcttattgaccacatcttactttttgtgctcataatttctggaatcaa-attgcagCCAAGGCTACTTGATGAGAAGCAGACAACTATTTACAAAAGAGCAGTCGACAAGAGCTATCATTTGAAGATGAAAGCTTCTAGATTCATTTTCAGTGAGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|169080642|gb|FE490972.1|FE490972
EST:     AGAACGAGGTCTACGCCATTGACATCGTCACAAGCACCGGTGAAGGAAAG                         CCGAAGCTTCTCGACGAGAGACAGAACA-CAGTCTAC
genomic: AGAACGAGGTCTATGCCATTGACATCGTCACAAGCACTGGTGAAGGAAAGgtttgtagct ... gttatcgcagCCGAAGCTTCTCGACGAGAGACAGA-CAACAGTCTAC
EST: gi|169069244|gb|FE480029.1|FE480029
EST:     AGAACGAGGTCTACGCCATTGACATCGTCACAAGCACCGGTGAAGGAAAG                         CCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGG
genomic: AGAACGAGGTCTATGCCATTGACATCGTCACAAGCACTGGTGAAGGAAAGgtttgtagct ... gttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGG
EST: gi|169055673|gb|FE465304.1|FE465304
EST:     AGAACGAGGTCTACGCCATTGACATCGTCACAAGCACCGGTGAAGGAAAG                         CCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGAC
genomic: AGAACGAGGTCTATGCCATTGACATCGTCACAAGCACTGGTGAAGGAAAGgtttgtagct ... gttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGAC
EST: gi|169065059|gb|FE474507.1|FE474507
EST:     AGAACGAGGTCTATGCCATTGACATCGTCACAAGCACTGGTGAAGGANAG                         CCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGAC
genomic: AGAACGAGGTCTATGCCATTGACATCGTCACAAGCACTGGTGAAGGAAAGgtttgtagct ... gttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 33 (upstream exon), 35 (downstream exon)
Score: 30

ACTGAGTGTTTCGAATCCGGAAACCCGCGTGGACGAGTCAGAGTTCGAGGAGAACGAGGTCTATGCCATTGACATCGTCACAAGCACTGGTGAAGGAAAGgtttgtagct ... gttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA
  ctttgac  putative branch site (score: 30)
                  aaatctaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtttgtagctttgacttatcgttgaaatctaaggaagtaagaaacgcaaacgttatcgcagCCGAAGCTTCTCGACGAGAGACAGACAACAGTCTACAAAAGGGCCGTGGACAAGAGCTATCATTTGAAGATGAAGGCCTCGAGGTTTATATTCAGTGAAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG