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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagttcatagattgagatcttcttcttgcttctttagcctgagtgggagtaactgagattgtttcaaaaccagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC

Basic information

species Selaginella moellendorffii
transcript EFJ10353
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: EFJ10353 (Selaginella moellendorffii), 3'ss of exon 3
lower sequence: GRMZM2G124434_T01 (Zea mays), 3'ss of exon 4
------------gtgagttcatagattgagatctt-----cttcttgcttctttagcctgagtgggagtaactgagattgtttcaaaaccagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
                 ||  | |  || |    |     | |     | |    ||||||     |    ||    |||| |    | ||||||| || |||||||| |||||||| || ||  ||||||||| ||| ||||| ||   |||||||||||||||||||||  | |||||  | ||||||||
gtgaacctactaccagttttctgggctgtggctgtgaggtcataacaatccaacggcctgataaaaaaatgctctctttgtct----atcagGTGGCCGCGAGAAGGCACCGGCTGCCTTCTGCAGAAAGGCTCAGACATCCACGGAGAGGTTTGAGATGTGGGGCGATGGTCTCCAGACCAGGTCCTTCAC

upper sequence: EFJ10353 (Selaginella moellendorffii), 3'ss of exon 3
lower sequence: GLYMA19G43400.1 (Glycine max), 3'ss of exon 4
------------------------------------------gtgagttcatagattga--gatcttcttct-tgcttctttagcctgagtgggagtaactgagattgtttcaaaac-cagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
                                           |||||  ||| | |    ||  ||||| | |     ||| |  | |    |  |         |||||    |   ||||||| | ||||||||||| ||||| |||||||| ||| | || |||||||  |||   |||||||||||||| |||||| |||| ||  ||||||||||
gtatgttcttgactgaattgcaagtttttgcagttgttgtatttgagtcaataaactagtggacattcttttgtagccttttggatttaacaagtttgttgctttgtgttttgtcatatagGTGGAAGGGAGAAGGCTCCTGCTGCTTTTTGTCGCAAGACACTTACTTCCAAAGATCGATTTGAGATGTGGGGAGATGGATTGCAAACAAGATCCTTCAC

upper sequence: EFJ10353 (Selaginella moellendorffii), 3'ss of exon 3
lower sequence: GLYMA10G30400.1 (Glycine max), 3'ss of exon 3
------------------------------------------------gtgagttcatagattgagatcttct-tcttgct---tctttagcctgagtgggagtaactgagattgtttcaaaac-cagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
                                                 || ||  || | | |  || || | | ||| |     ||  |  | | |  |  |   |    ||||||    |  |||||||| | ||||||||||| ||||| |||||||| || |   | |||||||| |||   |||||||||||||| |||||| |||| || ||||| || ||
gcatgtttttcactgaattgaaagcttttatagttttgactttaccaaatgggtaaattggtagtcattttatattttggtaacctttcggatttaataagtttgtgttttgttgttttgtcatacagGTGGAAGGGAGAAGGCTCCTGCTGCTTTTTGTCGTAAAGTAATCACTTCCACTGATAGATTTGAGATGTGGGGAGATGGATTGCAAACACGATCATTTAC

upper sequence: EFJ10353 (Selaginella moellendorffii), 3'ss of exon 3
lower sequence: GLYMA20G36740.2 (Glycine max), 3'ss of exon 4
------------------------------------------------gtgagttcatagattgagatcttct-tcttgct---tctttagcctgagtgggagtaactgagattgtttcaaaac-cagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
                                                 || ||  || | | || || || | | ||| |     ||| |  | | |  |  |   |    ||||||    |  |||||||| | ||||||||||| ||||| |||||||| || | | | |||||| | |||   |||||||||||||| |||||| |||| || ||||| || ||
gcatgctttacactgaattgaaaacttttatagttttgtctttaccaaatgggtaaattggtagacattttatgttttggtaaccttttggatttaataagtttgtgttttgttgttttgtcatacagGTGGAAGGGAGAAGGCTCCTGCTGCTTTTTGTCGTAAAGTTATCACTTCCTCTGATAGATTTGAGATGTGGGGGGATGGATTGCAAACACGATCATTTAC

upper sequence: EFJ10353 (Selaginella moellendorffii), 3'ss of exon 3
lower sequence: AT5G28840.1 (Arabidopsis thaliana), 3'ss of exon 4
-------gtgagttcatagattgagatcttcttct---tgcttctttagcc-tgagtgggagtaac-tgagattgtttcaaaaccagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
            |  | || ||  |  |||  | |   || ||  ||       | ||||| |||  |  | |||  ||||    ||||||| | ||||||||||| ||||| || ||| | ||||||| ||||||||| |||  ||||||||||||||| |||||  | ||||| || || || ||
gtagtatacagataaacagtttccgtactttgtgtgaatgattagttgtttactattgggattaatatttgcttggatcaac-ttagGTGGAAGGGAGAAGGCTCCAGCTGCTTTCTGTAGGAAGGCTCAGACTTCCACTGATAGGTTTGAGATGTGGGGAGATGGGCTTCAGACCCGTTCTTTTAC

upper sequence: EFJ10353 (Selaginella moellendorffii), 3'ss of exon 3
lower sequence: Vv14s0030g02180.t01 (Vitis vinifera), 3'ss of exon 4
-----------------------------------------------------------------------------gtgagttcataga---ttgagatcttctt----cttgcttctttagcctgagtgggagtaactgagattgtttcaaaac--------cagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
                                                                                ||| || |||   || ||  |   ||      |   || |     ||  |   ||||| |  | | ||||    |          ||||||  ||||||||||||| ||||| ||||| ||||||||||| |||||||||||   |||||| |||||||| || ||| | || || || || |||||
gtatgctcttaaaagttatggtgattttctacatgtattcgtgattttgtctcttggtcaccgtcaatggtagggagccaagtgcagagaggtttaagcccgctttaaattctaaatcatatttttggatcttagtaattttg-tcgttttggtatatttgtcgtagGTGGGAGAGAGAAGGCTCCTGCTGCTTTTTGCCGAAAGGCTCTCACTTCCACCGACAAGTTTGAAATGTGGGGAGACGGACTTCAAACTCGTTCTTTCAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 33 (upstream exon), 30 (downstream exon)
Score: 4

TGCACTGAAGAGCTGTGCAAGCACTACACCAAGGATTTTGGGATCGAGTGCCGCATTGGACGATTCCACAACATCTATGGACCCTTTGGAACCTGGAAAGgtgagttcat ... tcaaaaccagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cttcttcttgcttctttagcctgagtgggagtaactgagattgtttcaaaaccagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC
                            gagtaac  putative branch site (score: 4)
                                        ttgtttc  putative PPT
                                           tttcaaaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagttcatagattgagatcttcttcttgcttctttagcctgagtgggagtaactgagattgtttcaaaaccagGTGGACGAGAGAAGGCTCCGGCTGCGTTTTGTCGAAAGGCTCTGACTTCCACCGATTCGTTTGAGATGTGGGGCGATGGAATGCAGACGCGATCCTTCAC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GATGGA