Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   		6  
 5'  3'   		7  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT

Basic information

species Selaginella moellendorffii
transcript EFJ25874
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: LOC_Os12g14100.1 (Oryza sativa), 3'ss of exon 1
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
             | |  ||   | ||||      |   ||||   ||  || |||  || | |         | | |     |||||||||  ||  ||||| || |||||   ||   |  |  | |||||||||||||| || |||||||||||||| |||||||| ||||||||    || ||||||  | | || 
------------gtacataatt-cattcatcatttctccttctc--catttcattt--catcgtccatggatgagtgttcgattttgtttttttttgtagGGGTCCGGGGGTGGCGGCGATGATCATGTCGTGGGTGATCACGCTGTACACGCTGTGGCAGATGGTGGAGATGCACGAGTGCGTGCCGGGGCGGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: LOC_Os08g03350.1 (Oryza sativa), 3'ss of exon 2
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttt-tcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
         |  |  |  |  | |||   || |  ||| |    ||  ||        | |       ||  ||  | |  || || ||   |  || ||||||||| ||  ||  ||| ||  | || || ||| | ||||| ||||||||||||||||| |||||||| |||||||| ||||| || |||||| | || 
---------gtcagtcctactatcttcttcatcttcatctgaacgaagaacacacgaacgaaccaagaaattaatctaactgaattcttgaatttttgcagGGGACCTGGCATCGCGGTGCTGATCCTGTCATGGATCATCACGCTCTACACGCTGTGGCAGATGGTGGAGATGCACGAGATGGTGCCCGGGAAGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G180659_T02 (Zea mays), 3'ss of exon 3
--------------gtgtcacccgca-atcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgt---ttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
              | | ||       || ||  |||    ||   | |   |     |    | |||| |  | ||  | ||      |  | | |   |||   | | |  | |||||| || ||  ||  ||| || ||  | |||||||||||||| || |||||||||||||| |||||||| |||||||| |||||||| |||||| | || 
gtacgtacgtgtccgagccatggatggatgctggagagagctc---ggctggtacatatatatagtcgtctgacgaccctaccccatccccgtcatgtcatgtcgatctgcactgcagGGGCCCTGGCATCGCGGTGCTGGTGGTGTCGTGGGTGATCACGCTGTACACGCTGTGGCAGATGGTGGAGATGCACGAGATGGTCCCCGGGAAGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G127328_T01 (Zea mays), 3'ss of exon 2
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
||  | | ||||    ||||  | |    ||   ||||  |  ||| |    ||      |||| ||      | | |||  | |    | | | | ||||||  | ||  ||  | |  |  | || |||||||||||||| || |||||||||||||| |||||||| |||||||| ||||| ||||||||| | || 
gta-cgcacgcatcaatcaatcagtcat-catttccattctgatgatagga-gt------acttgtc---atgaatgtaactactg--ctgtggattcagGGGTGCCGGCATCACGATCATGCTCCTGTCGTGGGTGATCACGCTGTACACGCTGTGGCAGATGGTGGAGATGCACGAGATGGTGCCGGGGAAGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G046743_T01 (Zea mays), 3'ss of exon 2
--gtgtcacccgca-atcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
    |    | ||| ||| ||  |  | || || |    | |      |||||     |   |||||||    |    |  || |   | | |       |||||| || ||  ||   || || ||  | |||||||||||||| || |||||||||||||| |||||||| |||||||| ||||| || || ||| | || 
gtacgcacacggcacatcttcgcgcgcgtt-acggtt--acggcgaccacaaacaaaatg--aacttttttttgttg-tcaaatggcatatatg------cagGGGCCCTGGCATCGCCGTGCTGGTGGTGTCGTGGGTGATCACGCTGTACACGCTGTGGCAGATGGTGGAGATGCACGAGATGGTGCCCGGAAAGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G127338_T02 (Zea mays), 3'ss of exon 1
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
|||    |   |     | ||   | |  | |   | ||       |  | ||   |  || |      | ||  | | | |   ||  |     | |||||| || ||  ||  ||| ||  | || |||||| | ||||| || |||||||||||||| |||||||| |||||||| ||||||||||| ||| | || 
gtgcgtacgctagcttgctagctgtgtgcctgtgtc-tgactgactctgagcgcgcttaaattagatgtatca--tatcatgccatgcatgcatatgcagGGGCCCCGGCATCGCGGTGCTGCTGCTGTCGTGGATCATCACGCTGTACACGCTGTGGCAGATGGTGGAGATGCACGAGATGGTCCCGGGCAAGCGCTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G127294_T01 (Zea mays), 3'ss of exon 2
------gtgtcacccg--caatcctcaagaactttcacagccccatgttc--ttgacaaatcgttttccaacttttccaagcaactttaaagatt--tgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
        |||  |||     | ||| |  | |  |||   |    | ||  |  |  |  | |  |||| || | | |  |   | | ||  |    |  |  | || |||||     || |||  ||| || || || | |||||  ||||| ||||||||||||||||| |||||||| ||||||||    |||||||||||| | || 
gtccgtccgtcgtccgactcgtgctctactaatcacaccatcgatcgatcgatccatcagccatcatccatctgt-cgatccggttctcaatctcgacggatcgccttgcagGGAGGTCGGCGTCACGGTGCTGGTTCTGTTGTGGGCCATCACGCTCTACACGCTGTGGCAGATGGTGGAGATGCACGAGTGCGTCCCGGGGAAGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G175321_T01 (Zea mays), 3'ss of exon 1
-------------------------------------------------------------gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttc-caagcaactttaaagatttgtttttcgt-tccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
                                                              |||  |  |||  | ||          |  |  |||| |   |||   || | | |     | | |    |  |    |   |          |  | |||||| || || ||  | |   | || || || ||||||||||| || |||||||||||||| ||||| || ||||||||    ||||| |||||| | || 
gtacgtggcatccatctgatctggtcttaattgttgtcctcgcctcgatcggcatgcaatattgtgccttgcatgcatctgtcgtcgcgatcgatccatttaactgatcgatggatatatggatctgcatgcgtgaacgaactaacacaccgcatgcgtgcagGGGTCCCGGCGTGGTCGCCATGGTCCTGTCTTGGGTGATCACCCTGTACACGCTGTGGCAGATGGTCGAGATGCACGAGTGCGTCCCCGGGAAGCGTTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GRMZM2G429322_T01 (Zea mays), 3'ss of exon 2
-----------------------gtgtcacccg-caatcctcaagaacttt------cacag-ccccatgttcttgacaaatcgttttccaactt--ttccaagcaact--ttaaagatttgtttttcg--ttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
                        | ||  ||  | || |  | |  || |      || ||       | | | |    || | |   |  |||  |||| | |  |      |  |     ||||||  | |||||||    || |||  ||| || || || |||||||| |||||  | |||||||||||||| |||||||| ||||||||    || ||||||||| | || 
gtccgtctcgtcatccaccatatatatcttccatccatgcatatgggctctgccaagcatagattttgggctttcggtgcatggctcgtcgccttccttcctaccttccggccgatcaccgagttttcgattcccagGGGCGTGGGCGTCACGGTGCTGGTCCTGTCGTGGGTCATCACGGTGTACACGCTGTGGCAGATGGTGGAGATGCACGAGTGCGTGCCGGGGAAGCGGTTC

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GLYMA16G06740.1 (Glycine max), 3'ss of exon 1
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcg--ttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
 ||  ||      |  | |  |     || |     ||  | ||     |||  ||     |   || ||  || | |  | |||  | ||  |   |||||||| || |||||  || |||||||| | || ||| | |||||||||||||| || |||||||||||||| ||||| || |||||||| ||||| || |||
ctgctactttttttttttataagaaa-caaattataatagtattttggcatc-cttgagggccagtcaaaaaaattgttgatattaatattgtgatctccagGGGTCCTGGGGTGGTGATTCTTGTATTGTCATGGATAATCACACTCTACACACTATGGCAAATGGTGGAGATGCATGAGATGGTCCCTGGGAAAAGGTTT

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GLYMA11G37340.1 (Glycine max), 3'ss of exon 1
---gtgtcacccgcaa--tcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
   |  |||      |  |  ||   ||| |||    ||   || | || |   | | |  |      |||| || ||   |||   ||   ||     |   |||||  | ||   |   || ||| |  | || ||||| ||||| |||||||| |||||||||||||| || |||||||||||||| || || || ||||||
ttcggatcatgaattactttttcttaaaccttctt--ccatttgattttaattgaacatactt--gtgtttctaatcattattatc-atcatttaaaaataatagGGGTGCCGGTTCCACCGTGCTTATCTTGTCATGGGTAATCACCCTCTACACCCTGTGGCAAATGGTTGAGATGCACGAAATGGTGCCCGGAAAAAGATTT

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GLYMA18G01300.1 (Glycine max), 3'ss of exon 1
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
 | |     | |      || || |         || |    ||||       |   |  |||| |      ||   | |  ||   ||     |   ||||| || || |     || ||| |  | || ||||| ||||| |||||||| || ||||||||||| || |||||||||||||| || ||||| ||||||
atatgctttggatcatgaaataattcctctttaaccttccatttgattttatttgaacatacttgtgtttctaatcattattatcatttaaaaataatagGGGCCCCGGTGCTACCGTGCTTATCTTGTCATGGGTTATCACCCTCTACACCCTATGGCAAATGGTTGAGATGCACGAAATGGTGCCAGGGAAAAGATTT

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: GLYMA19G24540.1 (Glycine max), 3'ss of exon 1
---------gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
          | ||  |     |||  |  |  |||    | ||  | ||  | | |||  |||     |  ||   | |  | |||   ||| |  | |  ||| |||||| || || ||  || |||||||  | || ||| | |||||||||||||| ||||||||||||||||| ||||| || ||| |||| ||||| || |||
aacaactaattttcttcttttttccaaattattttttgtggtcc--tttttataagaaaaagtt-----atattcgtatgttaattttgtgatctcctgt--gttacagGGGTCCTGGTGTGGTGATTCTTGTGTTGTCATGGATAATCACACTCTACACACTGTGGCAAATGGTGGAGATGCATGAGATGATCCCTGGGAAAAGGTTT

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: AT5G40780.2 (Arabidopsis thaliana), 3'ss of exon 2
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
           ||| | |   |||    || |    || ||    | |     |||  ||| | ||  || |   |||||   ||||||||   || ||||||||| ||  |    ||  | ||  | || ||||| || ||||| |||||  | |||||||||||||||||||| |||||||| || || ||| | |||
--------gtaaattcccctaaaccaaaaccggtttattttacctaaaccggatttatcaaatctt--aaccggttttaa---tttgttttct-tttcagGGGACCGGGAATTGCAGTGTTGGTTTTGTCATGGGTCATAACACTATACACATTATGGCAAATGGTGGAAATGCATGAAATGGTTCCTGGAAAGCGTTTT

upper sequence: EFJ25874 (Selaginella moellendorffii), 3'ss of exon 2
lower sequence: PP1S5_176V6.1 (Physcomitrella patens), 3'ss of exon 2
------gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttc--caacttttccaagcaactttaaagatttgtttttcgt-tccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
      |   | | ||   |    ||    | ||| || | ||   | |         |||| |  ||       |||| |||      || | | | |||   | ||||||||| || ||  |||| || ||  | |||||| ||||||| || |||||||| ||||| ||||||||||||||||| ||||||  ||||||| | || 
gtgagggctcctcacgttttgtctaatccgtctcagagtcacacagtttcatgttgcagtttgcggcagtgggcacaagtaac-----ggaatggctattcacatgcagGGGACCGGGAGTGGTGGTGCTGGTGGTGTCGTGGATGATCACGCTGTACACGCTCTGGCAGATGGTGGAAATGCACGAGATGGTCGAGGGGAAGCGTTTC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|169100469|gb|FE502683.1|FE502683
EST:     GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTG                         GGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
genomic: GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTGgtaagttctt ... ttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
EST: gi|169053550|gb|FE463319.1|FE463319
EST:     GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTG                         GGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
genomic: GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTGgtaagttctt ... ttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
EST: gi|169083876|gb|FE492788.1|FE492788
EST:     GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTG                         GGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
genomic: GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTGgtaagttctt ... ttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
EST: gi|169072771|gb|FE484349.1|FE484349
EST:     GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTG                         GGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC
genomic: GTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTGgtaagttctt ... ttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 22

CCAGGAACGCCAAGTGGTGGTACTCGGCCTTCCATAATGTCACCGCCATGGTTGGAGCTGGAGTTCTTAGCCTCCCCTCGGCGATGGTCTATCTTGGCTGgtaagttctt ... ttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT




<











<


<











 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

acaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT
 tcttgac  putative branch site (score: 22)
                                      tttgtttttcgttcc  putative PPT
                              tttaaagatttgtttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgtcacccgcaatcctcaagaactttcacagccccatgttcttgacaaatcgttttccaacttttccaagcaactttaaagatttgtttttcgttccagGGGACCAGGGGTCCTGGTTCTTGTACTCTCGTGGGTGATCACACTCTACACGCTGTGGCAAATGGTGGAAATGCACGAAATGGTCCCGGGGAAGAGATTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGA
- - -acccgca
- - - - - - - - - - - -ctttcac
- - - - - - - - - - - - - - - agcccca
- - - - - - - - - - - - - - - - - - -tgttctt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tttgttt