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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtacacatttttcttttaagtcatccctctcttaatactctaggattcagtttgtgacttggaacattagtgtaatcgttctactcgtagtagtttgatttgctccctaatctattttgcatttccatctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACTGTGACTAGTCCTGAAGACACAATTAGACAGTGCTCCGAGCTTTTTCTGT

Basic information

species Physcomitrella patens
transcript PP1S137_89V6.1
intron # 8
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|37827978|gb|BJ585990.1|BJ585990
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37836492|gb|BJ594500.1|BJ594500
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|67695499|gb|BJ955732.1|BJ955732
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208364031|gb|DC950379.1|DC950379
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208364746|gb|DC954476.1|DC954476
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37828269|gb|BJ586281.1|BJ586281
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208370169|gb|DC944394.1|DC944394
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208386575|gb|DC952770.1|DC952770
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208347945|gb|DC921202.1|DC921202
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208368085|gb|DC947645.1|DC947645
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37834823|gb|BJ592835.1|BJ592835
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162275643|gb|FC451645.1|FC451645
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162211194|gb|FC386906.1|FC386906
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208362674|gb|DC947165.1|DC947165
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162201426|gb|FC377152.1|FC377152
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208380771|gb|DC919459.1|DC919459
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37837928|gb|BJ595936.1|BJ595936
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208357863|gb|DC918625.1|DC918625
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37851718|gb|BJ609726.1|BJ609726
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37832314|gb|BJ590326.1|BJ590326
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208360951|gb|DC946640.1|DC946640
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|18338754|gb|BJ170778.1|BJ170778
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208346101|gb|DC917054.1|DC917054
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208356208|gb|DC946256.1|DC946256
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|18334781|gb|BJ166798.1|BJ166798
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208366441|gb|DC947609.1|DC947609
EST:     TTGAGAACGAATTTTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208385973|gb|DC956811.1|DC956811
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162250055|gb|FC427364.1|FC427364
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208358539|gb|DC946500.1|DC946500
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208360140|gb|DC950169.1|DC950169
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162250054|gb|FC427363.1|FC427363
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162243889|gb|FC423206.1|FC423206
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37827898|gb|BJ585910.1|BJ585910
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208399394|gb|DC924824.1|DC924824
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|208397251|gb|DC920880.1|DC920880
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37844098|gb|BJ602106.1|BJ602106
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|162208934|gb|FC385278.1|FC385278
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37844701|gb|BJ602709.1|BJ602709
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
EST: gi|37826210|gb|BJ584222.1|BJ584222
EST:     TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAG                         GGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT
genomic: TTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 34 (downstream exon)
Score: 11

GTGTTTCCCAAGGGATGGGATAAGACATTCTGCCTGCAATTCGTTGAGAACGAATTCTCCGAGATCCATTTCTTTGGTGACAAAACCTACCAGgtacacattt ... ctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACTGTGACTAGTCCTGAAGACACAATTAGACAGTGCTCCGAGCTTTTTCTGT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ctcgtagtagtttgatttgctccctaatctattttgcatttccatctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACTGTGACTAGTCCTGAAGACACAATTAGACAGTGCTCCGAGCTTTTTCTGT
                     ccctaat  putative branch site (score: 11)
                               ttttgcatttccatct  putative PPT
                        taatctatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtacacatttttcttttaagtcatccctctcttaatactctaggattcagtttgtgacttggaacattagtgtaatcgttctactcgtagtagtttgatttgctccctaatctattttgcatttccatctctttctagGGTGGCAACGACCACGAAATCTTTGAGTCCGAGAAGACCATCGGTCACACTGTGACTAGTCCTGAAGACACAATTAGACAGTGCTCCGAGCTTTTTCTGT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCTT