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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgatgctgcacttcacctagggctggcctagttctgtgtatcactagcaagttttgtactgctccttgacatggtggcaagtggggctgtgcaactcctaccattcacttacgcataggctttttggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG

Basic information

species Physcomitrella patens
transcript PP1S32_198V6.1
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S32_198V6.1 (Physcomitrella patens), 3'ss of exon 7
lower sequence: GLYMA18G02440.1 (Glycine max), 3'ss of exon 9
gtgatgctgcacttcacctagggctggcctagttctgtgtatcactagcaagttttgtactgctccttgacatggtggcaagtggggctgtgc-------aactcctaccattcacttacgcataggctttttggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG
                                             ||  || ||| ||        |  | |   | |||  ||  ||| |         || | |||| |  ||      |       |  |  |  ||  ||| |||||  || |||||||||| || ||||| ||||||||||| ||||| |||||||| || ||||||||| | |  || |||||||
--------------------------------------------gtaagaaatttggttgaa----taaaaaaaattgcatatgaagctttcttttccaaaatttctacaaaacatggtaactgtttactcctataaattttagAGCTCGGTATTCTAGTTGGGAAAGGAGGGCTGCATGGAAATGTTTTTAGGATCAAGCCACCAATGTGTTTCACCAAAGATGATGCAG

upper sequence: PP1S32_198V6.1 (Physcomitrella patens), 3'ss of exon 7
lower sequence: GLYMA11G36000.2 (Glycine max), 3'ss of exon 7
gtgatgctgcacttcacctagggctggcctagttctgtgtatcactagcaagttttgtactgctccttgacatggtggcaagtggggctgtgcaactcctaccattcacttacgcatag--gcttttt------ggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG-------------
                                             ||  || ||| ||        |  | |   | |||  ||  ||| | |   ||| |   |||    |  |||||   || |||        |  ||| ||| |||||  || ||||||| || || ||||| ||||||||||| ||||| |||||||| || ||||||||| | |  || |||||||             
--------------------------------------------gtaagaaatttggttgaa----taaaaagaattgcatatgaagcttt-cttttccaaaatttctacaaaacatagtaactgtttactcctataaattgtagAGCTCGGTATTCTAGTTGGAAAAGGAGGGCTGCATGGAAATGTTTTTAGGATCAAGCCACCAATGTGTTTCACCAAAGATGATGCAGGTATATAAATAAA

upper sequence: PP1S32_198V6.1 (Physcomitrella patens), 3'ss of exon 7
lower sequence: GLYMA11G36000.3 (Glycine max), 3'ss of exon 7
gtgatgctgcacttcacctagggctggcctagttctgtgtatcactagcaagttttgtactgctccttgacatggtggcaagtggggctgtgcaactcctaccattcacttacgcatag--gcttttt------ggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG
                                             ||  || ||| ||        |  | |   | |||  ||  ||| | |   ||| |   |||    |  |||||   || |||        |  ||| ||| |||||  || ||||||| || || ||||| ||||||||||| ||||| |||||||| || ||||||||| | |  || |||||||
--------------------------------------------gtaagaaatttggttgaa----taaaaagaattgcatatgaagcttt-cttttccaaaatttctacaaaacatagtaactgtttactcctataaattgtagAGCTCGGTATTCTAGTTGGAAAAGGAGGGCTGCATGGAAATGTTTTTAGGATCAAGCCACCAATGTGTTTCACCAAAGATGATGCAG

upper sequence: PP1S32_198V6.1 (Physcomitrella patens), 3'ss of exon 7
lower sequence: Vv07s0031g01330.t01 (Vitis vinifera), 3'ss of exon 7
gtgatgctgcacttcacctagggctggcctagttctgtgtatcactagcaagttttgtactgctccttgacatggtggcaagtggggctgtgcaactcctaccattcacttacgcataggctttttggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG
                                                   ||||   ||| | || |||    |    || |     |||      || ||     |||||   ||    ||||       || |||  ||||  || |  | || ||||| || || |||||||||||||| ||||| ||||| |||||||||||| | |  || ||||| |
---------------------------------------------------gtttgcaactccaccatgattaagaaaaaattaa--ttgtaatttaccaacacactacttatttattaatttttaa----ctgtagAGATCGGTATTCTTATCGGAAAGGGAGGACTACATGGAAATGTCTTCAGAATAAAGCCACCTATGTGTTTCACCAAAGATGATGCTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67576495|gb|BJ949319.1|BJ949319
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|162186136|gb|FC361340.1|FC361340
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|162234389|gb|FC410135.1|FC410135
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|37844993|gb|BJ603001.1|BJ603001
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|208398602|gb|DC916123.1|DC916123
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|37823581|gb|BJ581647.1|BJ581647
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|162183482|gb|FC358774.1|FC358774
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|37848881|gb|BJ606889.1|BJ606889
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|37828828|gb|BJ586840.1|BJ586840
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
EST: gi|67700022|gb|BJ960255.1|BJ960255
EST:     CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAG                         ACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA
genomic: CAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 32 (upstream exon), 29 (downstream exon)
Score: 11

CGGGGCCGGGGTCTCATGCTGGGCGTTGAGTTGGTAAAAGACCGAACAACCAAGGCTCCAGCGAAAGAAGAGACTCTTCTTCTTTTTGAGCAACTGAAAGgtgatgctgc ... gatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tggggctgtgcaactcctaccattcacttacgcataggctttttggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG
                        cacttac  putative branch site (score: 11)
                                       ttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgatgctgcacttcacctagggctggcctagttctgtgtatcactagcaagttttgtactgctccttgacatggtggcaagtggggctgtgcaactcctaccattcacttacgcataggctttttggatgttgcagACCTCGGAGTTTTAGTTGGGAAGGGCGGGCTTCATGGAAATGTCTTTAGAATCAAGCCTCCTATGTGTTTCTCAATGGAAGATGCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG