ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgcttgctccgagataattcggaatttgttgtagatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC

Basic information

species	Physcomitrella patens
transcript	PP1S15_262V6.1
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: PP1S15_262V6.1 (Physcomitrella patens), 3'ss of exon 5
lower sequence: LOC_Os03g57130.1 (Oryza sativa), 3'ss of exon 5

gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgc-ttgctccgagataattcggaatttgttgtagatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC
                                                                                            |   ||  |||||    |||   |||||   |    | |  || |||||    || |  |    ||| |    |||   | ||        | ||   || || ||||| |||||  ||||||| |||   ||||| ||   ||| || || |||||||||||||| || |||||||| ||||| ||||||||||| || || 
--------------------------------------------------------------------------------------------tcgatgatttattaatgatcaagttgcttttaccagactttgattttgtcaatga-gcatactcaataagttaattcggttttg------ttttgcaatatatgcagGTTGATGATTCTGAAGTCATTGGGTTTCTGCTGCAAACTGAAATAATTCCTCTGTGTCTCCGTACTATGGAGATGGGCAGTGAACTTTCGAAAACG

upper sequence: PP1S15_262V6.1 (Physcomitrella patens), 3'ss of exon 5
lower sequence: GLYMA08G43220.1 (Glycine max), 3'ss of exon 5

gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgcttgctccgagataattcggaatttgttgtagatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC
                                                                                              | ||  || ||  |  |   ||  |   |  | ||     || | || | | | ||||   |||   || ||||| | |      || | |  |  ||    |||| ||||| || |||||||| | ||| || || ||||||||||| ||||| ||||| || || || ||||||||||| |||||||| ||||| || 
----------------------------------------------------------------------------------------------attgt-ttcttgaattttttttttttgtaattcatatttcatctattcttgtttttttatcaattgtgacattttttttctgtctgatttttattcac---tagGTTGATGATACAGAAGTTATAAGTTTCCTTCTTTCAACAGAGATAATTCCCCTGTGCCTGCGCACCATGGAAATGGGCAGTGAACTATCCAAAACA

upper sequence: PP1S15_262V6.1 (Physcomitrella patens), 3'ss of exon 5
lower sequence: GLYMA18G10400.1 (Glycine max), 3'ss of exon 5

gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgcttgctccgagataattcggaatttgttgtagatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC
                                                                                |||    | ||||||     || ||||  ||| ||   ||       ||    | | | ||||     ||||  ||||   || ||||| |  |  | |   | | ||| ||   ||||| ||||| || |||||||| | ||| || || ||||||||||| |||||||| ||  | ||||| ||||||||||| ||||| || ||||| || 
-------------------------------------------------------------------------------attatcattgtttttaaatttttttttgtaatccctatttc-------atct--attcttttgt-----ttttgttaattctgacatttt-tcctggctgatttttattc-ac---cagGTTGATGATACAGAAGTTATAAGTTTCCTTCTTTCAACAGAGATAATTCCTCTATGCTTGCGAACCATGGAAATGGGCAGTGAGCTATCCAAAACA

upper sequence: PP1S15_262V6.1 (Physcomitrella patens), 3'ss of exon 5
lower sequence: GLYMA02G46250.1 (Glycine max), 3'ss of exon 5

gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgcttgctccgagataattcggaatttgttgtagatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC
                                                                       | |||  | | |     || |||  |   ||  |||   || | ||  |    ||| || |   ||||| ||   |  |||            ||||| | || |        | ||  ||   ||||| ||||| || || ||||| | ||| || || ||||||||||||||||| ||||| || || | |||||||||||| |||||| | || || || 
-----------------------------------------------------------------------ctagtgggatgaagctattgttttttttgttgcttg---tcacatgtacaattatactt-gagttttgtcgtgtttaattt------------attttctgttga--------tatttcac---cagGTTGATGATACAGAGGTTATAAGTTTCCTTCTTTCAACAGAGATTATTCCGCTGTGCCTGCGCAGTATGGAAATGGGCAGTGAATTGTCAAAAACT

upper sequence: PP1S15_262V6.1 (Physcomitrella patens), 3'ss of exon 5
lower sequence: AT3G20800.1 (Arabidopsis thaliana), 3'ss of exon 5

----gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgcttgctccg-agataattcggaatttgttgtagatgtttttata----atgaagagattttatattaacgggactctgttctacatac-agGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC
       ||| | ||  ||  | ||  ||     |     |      |  |||| |    ||    |    |||   |  ||    ||| |   ||     |  ||| | | | | | ||     | ||| ||||   | | | || | |  |||  | ||    || |  |   || ||   | |   | |    | |   | | |||| ||||| || || || ||||  || || || ||||| || |||||||||||||| || || || ||||||||||||||||| || || || || 
gtacaccatcccgtcccttttctgttccttgtttctc---tttggttttgatgttccccacctactttttgctga--aagcaaaggactaaaaatt-----gtgttagtggtcttggtttctaaagtagagaatttatattcttttttggtggttagtgtatgaaataagaaaggttcatcggatccttatttcactttgttttccagGTTGATGATACAGAGGTCATTAGCTTCCTTCTTTCAACTGAAATTATTCCTCTGTGCCTCCGTACCATGGAAATGGGGAGTGAGCTGTCAAAAACA

upper sequence: PP1S15_262V6.1 (Physcomitrella patens), 3'ss of exon 5
lower sequence: Vv19s0090g00930.t01 (Vitis vinifera), 3'ss of exon 7

gtgatcacttctattgccagtcgctcagaaccggagtggaagctgaatgtgcaggccccgtatcggtctgcccagtcagtttacttatttttttagtgcattatttgacatcgcttgctccgagataattcggaatttgttgtagatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC
                                                                                        ||  ||  |  ||| ||||  || | |||       |   || |    ||  | | |   ||   |  ||     | | |||  ||        | |   ||   | ||||| ||||| || |||||||||| ||| || ||||||||||| || ||||| ||||| ||||| || ||||| |||||||||||| | || || || 
---------------------------------------------------------------------------------------attaattgctatattgtttg-tattgtttgt------aaggtttg----ttactcttgtgattccaagtattgctggttattaccttttttattttttcccctctttccagGTTGATGATACAGAAGTTATTAGTTTCCTTCTCTCAACAGAAATAATTCCGCTGTGCCTACGCACCATGGAGATGGGGAGTGAATTGTCAAAAACA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|18356452|gb|BJ188511.1|BJ188511

EST:     TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAG                         GTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAG
genomic: TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAGgtgatcactt ... ctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAG

EST: gi|208348349|gb|DC905549.1|DC905549

EST:     TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAG                         GTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCT
genomic: TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAGgtgatcactt ... ctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCT

EST: gi|100356823|gb|BY965298.1|BY965298

EST:     TCGAGTATCTTCGATTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAG                         GTGGATGACACTGAAGTTATTTATTTTCTACTCTCAACAGAGATTATTCCT
genomic: TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAGgtgatcactt ... ctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCT

EST: gi|100368244|gb|BY948190.1|BY948190

EST:     TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAG                         GTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCT
genomic: TCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAGgtgatcactt ... ctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 9 (upstream exon), 32 (downstream exon)
Score: 2

TCCTCTTTATCTCTATCCATTTCTGAACACAGTAAGCAAGACAAGACCGTTCGAGTATCTTCGACTTACGAGCCTAGGAGTCATTGGTGCTCTTGTTAAGgtgatcactt ... ctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

agatgtttttataatgaagagattttatattaacgggactctgttctacatacagGTGGATGACACTGAAGTTATTAATTTTCTACTCTCAACAGAGATTATTCCTCTGTGTCTACGAACTATGGAAATGGGGAGTGAACTTTCCAAGACC