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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagtatgattcttagctcgtacttttaccaagtgcattatgtatgggtcgtgcaacgaggtaactttattcttttcgctttgaatgacctttgattaatctcggagccttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTGGTAGTGGTGTCACCAGATGTTGGGGGAGTAGCTCGTGCCCGTGCATTTG

Basic information

species Physcomitrella patens
transcript PP1S42_193V6.4
intron # 4
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|100383515|gb|BY950299.1|BY950299
EST:     CAGTNCA-TGGGATATTTTGACATTTCCGTTGATNCACGTT-ATGGGCAG                         CCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
genomic: CAGT-CAATGGGATATTTTGACATTTCCGTTGAT-CACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
EST: gi|100356373|gb|BY946332.1|BY946332
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAAAACTCGACTACCTGGCAAGTAAA-TGATATCATCAAACGACTTG
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
EST: gi|100399937|gb|BY990696.1|BY990696
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
EST: gi|162261958|gb|FC436817.1|FC436817
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAATACTCGACTACCTGGCAAGTANAATGATATCATCAAACGACTTG
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
EST: gi|100353033|gb|BY945573.1|BY945573
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAATACTCGACTACCTGGCA-GTAAAATGATATCATCAAACGACTT
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTT
EST: gi|208380333|gb|DC907658.1|DC907658
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAACCGACTNG
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
EST: gi|100372888|gb|BY948750.1|BY948750
EST:     GCAGTCAATGGGATATTTTGACATTTCCCGT-GATCACGTTTATGGTCAG                         CCTGGTA-TACTCGACTACCCTGGCA-GTAAA-TGATATCATCAAACGACT
genomic: GCAGTCAATGGGATATTTTGACATTTCC-GTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTG-TAATACTCGACTACC-TGGCAAGTAAAATGATATCATCAAACGACT
EST: gi|100353317|gb|BY964745.1|BY964745
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATC
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATC
EST: gi|162258694|gb|FC434389.1|FC434389
EST:     GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAG                         CCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG
genomic: GGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 33 (upstream exon), 35 (downstream exon)
Score: 25

AATCACTGAGGCTGGAGCCAATAGAGTGCTGGCTTGTGATCTTCATTCAGGGCAGTCAATGGGATATTTTGACATTTCCGTTGATCACGTTTATGGTCAGgtgagtatga ... ttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTGGTAGTGGTGTCACCAGATGTTGGGGGAGTAGCTCGTGCCCGTGCATTTG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ctttattcttttcgctttgaatgacctttgattaatctcggagccttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTGGTAGTGGTGTCACCAGATGTTGGGGGAGTAGCTCGTGCCCGTGCATTTG
                             gattaat  putative branch site (score: 25)
                          tttgattaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagtatgattcttagctcgtacttttaccaagtgcattatgtatgggtcgtgcaacgaggtaactttattcttttcgctttgaatgacctttgattaatctcggagccttgtgtgcagCCTGTAATACTCGACTACCTGGCAAGTAAAATGATATCATCAAACGACTTGGTAGTGGTGTCACCAGATGTTGGGGGAGTAGCTCGTGCCCGTGCATTTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGATG