Skip to another splice site:
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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ccttagagactacaaggttttggtcgttccaaccctggtcccatctttgccaatattagcaactaattttttgtttttttgaatgccacggtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACGAGTGCCCCGTGTGCACCCAGGTTTTTGAG

Basic information

species Physcomitrella patens
transcript PP1S100_61V6.1
intron # 4
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67728148|gb|BJ978407.1|BJ978407
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18368762|gb|BJ200847.1|BJ200847
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGAACGTGGTTCACTTTGG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGA-CGTGGTTCACTTTGG
EST: gi|162267194|gb|FC442606.1|FC442606
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|162209502|gb|FC386135.1|FC386135
EST:     TGTGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|162261088|gb|FC436949.1|FC436949
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67508592|gb|BJ941454.1|BJ941454
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67578360|gb|BJ950527.1|BJ950527
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18338320|gb|BJ170344.1|BJ170344
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18362314|gb|BJ194384.1|BJ194384
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67578398|gb|BJ950565.1|BJ950565
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|15262199|gb|BI437509.1|BI437509
EST:     CTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: CTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67691811|gb|BJ952044.1|BJ952044
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|208376809|gb|DC918750.1|DC918750
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCACGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|37848504|gb|BJ606512.1|BJ606512
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|37850045|gb|BJ608053.1|BJ608053
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67719884|gb|BJ970143.1|BJ970143
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|37853304|gb|BJ611312.1|BJ611312
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67701294|gb|BJ961527.1|BJ961527
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67691886|gb|BJ952119.1|BJ952119
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18367944|gb|BJ200028.1|BJ200028
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTT
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTT
EST: gi|18330512|gb|BJ162518.1|BJ162518
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGG
EST: gi|18372924|gb|BJ204526.1|BJ204526
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18359903|gb|BJ191964.1|BJ191964
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|208400671|gb|DC904857.1|DC904857
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCACGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|208390277|gb|DC949517.1|DC949517
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67695988|gb|BJ956221.1|BJ956221
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|15261952|gb|BI437262.1|BI437262
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|162209503|gb|FC386136.1|FC386136
EST:     TGTGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|37843440|gb|BJ601448.1|BJ601448
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|37851377|gb|BJ609385.1|BJ609385
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18363668|gb|BJ195740.1|BJ195740
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGA
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGA
EST: gi|162267193|gb|FC442605.1|FC442605
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|208365559|gb|DC935712.1|DC935712
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67701245|gb|BJ961478.1|BJ961478
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67571191|gb|BJ944015.1|BJ944015
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67727167|gb|BJ977426.1|BJ977426
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|37840814|gb|BJ598822.1|BJ598822
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67572616|gb|BJ945440.1|BJ945440
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|67508661|gb|BJ941522.1|BJ941522
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
EST: gi|18371213|gb|BJ202815.1|BJ202815
EST:     TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTG                         AGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG
genomic: TGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 16

GAAGCTCCTGCAATCATTGAGTCGAACTACGATGAACGGATCGTTGGATGTTCAGGAGGTCTCGGTGgtaaggttct ... gtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACGAGTGCCCCGTGTGCACCCAGGTTTTTGAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttgccaatattagcaactaattttttgtttttttgaatgccacggtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACGAGTGCCCCGTGTGCACCCAGGTTTTTGAG
               aactaat  putative branch site (score: 16)
      aatattagcaactaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ccttagagactacaaggttttggtcgttccaaccctggtcccatctttgccaatattagcaactaattttttgtttttttgaatgccacggtatggacagAGGACGAGCATGATGTGACGTGGTTCACTTTGGAGAAGGGCCAAACGCACGAGTGCCCCGTGTGCACCCAGGTTTTTGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAA