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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...aagttttattcttctctgaaactgtaactagaaaaaattatctggaagtggcagttcttatgctcatgtttgttttgtatcctttaatcactgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA

Basic information

species Physcomitrella patens
transcript PP1S12_178V6.2
intron # 20
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S12_178V6.2 (Physcomitrella patens), 3'ss of exon 20
lower sequence: LOC_Os12g04990.3 (Oryza sativa), 3'ss of exon 17
aagttttattcttctctgaaactgtaactagaaaaaat-tatctggaagtggcagttcttatgct---catgtttgttttgtatcctttaatcactgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
       ||| |  |  | || |  | |      || | ||| ||        |||| |  |  |    |  ||  |  |  ||  | |  | |  ||||  ||||| ||||||||||||||||||||||||||||||||||| || || || |||||||||||  | |||  |    |   ||||||| ||||||  ||  ||
----cacattttcttaggtaatttaagcccctggaactgtatttgatctgattagttttcttattaaataaattattaattgattgtgttttaatggtctcaagGAAAAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATAGCTCCCGAGAAGATTGAGAATGTCTATGCTAAGTGCAAGTTTATTGCGCAGTCCTTTATA

upper sequence: PP1S12_178V6.2 (Physcomitrella patens), 3'ss of exon 20
lower sequence: LOC_Os11g04980.1 (Oryza sativa), 3'ss of exon 18
aagttttattct---tctctgaaactgtaactagaaaaaattatctggaagtggcagttcttatgctcatgtttgttttgtatcctttaatcactgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
|  |||||  ||   | | | ||  | |  |     ||   | | | |       | || |  |  | |  |||  ||  |    |||  |   | |||  ||||| ||||||||||||||||||||||||||||||||||| || || || |||||||||||  | |||  |    |   ||||||| ||||||| ||  ||
attttttaggctatctattttaatttattcccctggaactctgtttcgtctgattacttttcttattaaattttaattgatgcaattttct---tttctcaagGAAAAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATAGCTCCCGAGAAGATTGAGAATGTCTATGCTAAGTGCAAGTTTATTGCGCAGTGCTTTATA

upper sequence: PP1S12_178V6.2 (Physcomitrella patens), 3'ss of exon 20
lower sequence: GRMZM2G059381_T03 (Zea mays), 3'ss of exon 5
aagttttattcttctctgaaac-tgtaactagaaaaaattatctggaagtggcagttcttatgctcatgtt-tgttttgtatcctttaatcactgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
  || ||  |||     |  || | |   |  ||  |||| | | |   || |   |   ||  |  |||  |   |||| |       |    |  || ||||| ||||||||||||||||||||||||||||||||||| || || || |||||||||||  | |||  |    |   ||| ||| | || || ||  ||
--gtgttggtctgactagttacatttttgtgtaattaattgtgttggtctgactagttacatttttgtgtaattaattgtttttcacttttctgggttgaagGAAAAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATAGCTCCAGAGAAGATTGAGAATGTCTATGCTAAGTGCAAGTTCATTGCTCAATGCTTTATA

upper sequence: PP1S12_178V6.2 (Physcomitrella patens), 3'ss of exon 20
lower sequence: GRMZM2G176546_T01 (Zea mays), 3'ss of exon 9
--------------------------------------------------aagttttattcttctctgaaactgtaactagaaaaaattatctggaagtggcagttcttatgctcatgtttgttt-tgtat--------cctttaatcact----gtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
                                                         | | |||  ||    | |   |     | ||| ||   | | |    ||  | |   | | ||| ||  | |||        |||| |||  ||    |  |  ||||| ||||||||||||||||||||||||||||||||||| || || || |||||||| ||  | |||       |   ||| ||| | || |||||  ||
gtaaaaacgtgcactgttttatattttatccattcagaacttgtgttcttgcagaacacttttccttggctatattgttgatttacattttccctaggcgctgtttgatctcaccgtttttcttcctatattattaatgccttaaatttctcctggattttagGAAAAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATAGCTCCAGAGAAGATTGAAAATGTCTATGCCAAATGCAAGTTCATTGCCCAATGTTTTATA

upper sequence: PP1S12_178V6.2 (Physcomitrella patens), 3'ss of exon 20
lower sequence: AT5G27600.1 (Arabidopsis thaliana), 3'ss of exon 18
aagttttattcttctctgaaactgtaactagaaaaaattatctggaagtggcagttcttatgctcatgtttgttttgtatcctttaatcactgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
  |||||||   ||    | | | |   ||        | |  |||||    ||||   |||      ||||||    |  ||| |||     |   ||||||||||||||| || ||||| || ||||||||||| || ||||| || ||||| || ||  |||||       || | ||  |||||||||||||| | 
--gttttataaatcaa-aacattcttgttatttctttctttgcggaagaaaaagttgaaatg------tttgtta---aaacttcaatgtgcatg-acagGAAGAAGAACATATTTAAGTTGGCGCAAGGAGAATATATAGCACCAGAGAAGATCGAAAATGTTTATACCAAATGTAGATTCGTTTCGCAGTGTTTCATT

upper sequence: PP1S12_178V6.2 (Physcomitrella patens), 3'ss of exon 20
lower sequence: Vv05s0020g03080.t01 (Vitis vinifera), 3'ss of exon 18
------------------------------------------------------------------------------------aagttttattctt----ctctgaaactgtaactagaaaaa---attat--ctggaagtggcagttcttatgctcatgtttgttttgtatcctttaatcactgtctg-cagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
                                                                                    | |||    ||||    || |||   ||| | |   | ||   | |||   |  |     || | | | |||   | || | |||||||     | |   | | || |||||||||||||||||| ||||| || |||||||| ||||| || || || || ||||||||  | |||  |    |    ||| || | ||||| || |||
gtaaagaccatatcatttttgttgacagttcttagacgaattaataatgactcctctctagttttggatgactcaattgactgtatgttcatatcttggtcctgtgattttgttattgatataatcgactataattacatacttcattacctgtgcatttcttagctttgtattaaa-agttgttatatgacagGAAGAAGAACATTTTTAAGTTGGCACAAGGAGAGTACATAGCTCCAGAGAAAATTGAGAATGTATATGCTAAATGCAAATTTGTTGCCCAGTGCTTTGTA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|162234487|gb|FC409518.1|FC409518
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|37847723|gb|BJ605731.1|BJ605731
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|162231381|gb|FC406804.1|FC406804
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|162272001|gb|FC448419.1|FC448419
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|162272000|gb|FC448418.1|FC448418
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|162159914|gb|FC336167.1|FC336167
EST:     CGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGGGAATACATTGCACCGGAAAA
genomic: CGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|37846424|gb|BJ604432.1|BJ604432
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
EST: gi|162192790|gb|FC369628.1|FC369628
EST:     GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAG                         GAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA
genomic: GGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 33 (upstream exon), 30 (downstream exon)
Score: 9

ACGGGAAATTTTTGATGAAGAAGGATGGTTACATACTGGCGATATTGGGTGTTGGTTGCCAGGTGGTCGTCTCAAGATCATTGACAGgtactttcga ... ctgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aagtggcagttcttatgctcatgtttgttttgtatcctttaatcactgtctgcagGAAGAAGAACATTTTCAAGTTAGCTCAAGGAGAATACATTGCACCGGAAAAGATTGAGAACATTTATCTTCGCAGTCGGTTTATTTCGCAGTGTTTCGTA
                                    ctttaat  putative branch site (score: 9)
                       tttgtttt  TA-rich tract