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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG

Basic information

species Physcomitrella patens
transcript PP1S372_38V6.1
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: LOC_Os11g06390.6 (Oryza sativa), 3'ss of exon 2
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcg---------cagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                  || | | ||  | ||| || ||||| | | |   || | ||  ||  |||  |       || | |   ||    |   || | | |||         |||||||| || || || |||||||| ||| ||||||||||||| || ||||||||||| ||  | |||||||| |||||||| || ||||| ||||| || |
----------------------------------------------------------------------------------gttcgtggt--gctcattcgtagtatt-tctttgtagcagttatccccatgtt-tatgtgatttaatcgtatactttacttttcttgcgaattgctggcagGCTGGGTTTGCTGGGGACGATGCGCCCAGGGCTGTTTTCCCTAGTATCGTGGGGCGCCCCCGTCACACCGGTGTGATGGTTGGTATGGGGCAGAAGGATG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: LOC_Os03g50885.1 (Oryza sativa), 3'ss of exon 1
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                  |||   ||| ||     |||  ||  | |     || ||     ||||||  | |     | |  || | ||||  ||||| | |    |||||||| ||||||||||| ||||| ||| ||||||| ||||||||||| || || || ||  | |||||||| || ||||| || ||||| ||||| ||||
----------------------------------------------------------------------------------gtaagctgtttggatc-tcagggtggtttccgtttaccgaaatgctgcat--ttcttggtagcaaaactgaggt--ggtttgtgt----cagGCTGGGTTCGCCGGAGATGATGCGCCCAGGGCTGTCTTCCCCAGCATTGTCGGCCGCCCTCGCCACACCGGTGTCATGGTCGGAATGGGCCAGAAGGACG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: LOC_Os12g06660.1 (Oryza sativa), 3'ss of exon 2
gtatgcatgcttaccgcat--atacgactgtaattctcccatgcaaatcgagcatgctaacaa-tcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
||   |||  | | |   |  || | | |      ||| ||   |   | |  |    |||||  |   ||||   ||         | |   |    | ||  | |  |  | |   |       ||  || |||||    | ||||  |||||                   ||||| || ||||| || ||||| || || ||||| || ||||| ||||||||||| || ||  ||||||| || ||||||||||| ||||| ||||| ||||
gtcatcatcatcatcaactcaatccaattaaccacctctcacaaatcacaacaacaacaacaaaccataccttatgttcttgcgttcaaaaaattcagtaaacaacaaca--acagcaacgataaaccattg-ataata---ataatccaaaatt-------------------cagGCCGGGTTCGCGGGCGACGACGCGCCGCGGGCGGTGTTCCCGAGCATCGTGGGTCGCCCCCGGCACACGGGGGTGATGGTGGGGATGGGGCAGAAGGACG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G047055_T01 (Zea mays), 3'ss of exon 2
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                              |||| |||   | |   |   ||   | |||| | || |||      ||| || ||| | | ||     | |  |  |   |          ||||||||| |||||||| ||||| |||||  |||| || |||||||||||||| ||||| ||| | |||||||| ||||||||||| ||||| ||||| || |
-----------------------------------------------------------------------------gtaagctac--cgccgatttgttccctgaactaaacacttat------tcttcagaatcccctaa---caacaccaacttctg----------gcagGCTGGGTTCGCCGGCGACGACGCCCCGAGGGCCGTCTTCCCCAGCATCGTTGGGCGCCCGCGCCACACCGGTGTGATGGTGGGGATGGGGCAGAAGGATG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G478779_T02 (Zea mays), 3'ss of exon 7
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                               | |  ||  |   | |   || ||| || ||| |     |||       || | |  ||  |     |       ||||||||| ||||| || ||||| |||||  |||| || |||||||||||||||||||| ||| | ||||| || || ||||| || ||||| ||||| ||||
----------------------------------------------------------------------------------------------gtgagccaccgg--tttggtcacatctgttctcggtg-gctgctggggccctaacaacagcaccaacctcg-----gcagGCTGGGTTCGCTGGCGACGACGCCCCGAGGGCCGTCTTCCCCAGCATCGTGGGGCGCCCGCGCCACACTGGTGTCATGGTCGGGATGGGGCAGAAGGACG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G152328_T02 (Zea mays), 3'ss of exon 1
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                              ||||  ||   || || |||    |  |  |    |   |  ||| ||| |     |||       || | |  |   |     |       ||||||||| ||||| || ||||| |||||  |||| || |||||||||||||||||||| ||| | ||||| || || ||||| || ||||| ||||| ||||
-----------------------------------------------------------------------------gtaagccac-cggtttggtcacatctgttcttgagaatgcaa-ggttctcggtg-gctgctggggccctaacaacaacaccaacctcg-----gcagGCTGGGTTCGCTGGCGACGACGCCCCGAGGGCCGTCTTCCCCAGCATCGTGGGGCGCCCGCGCCACACTGGTGTCATGGTCGGGATGGGGCAGAAGGACG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G478779_T01 (Zea mays), 3'ss of exon 5
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagt----actatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                       || | |  || ||| |  |  |  || | |  | |  || ||||    ||       | |   || ||| || ||| |     |||       || | |  ||  |     |       ||||||||| ||||| || ||||| |||||  |||| || |||||||||||||||||||| ||| | ||||| || || ||||| || |               
-------------------------------------------------------tatcca--gcccctcgtctgcgacaacggaactggcatggtcaaggtgagccaccggtttggtcacatctgttctcggtg-gctgctggggccctaacaacagCACCAACCTCG-----GCAGGCTGGGTTCGCTGGCGACGACGCCCCGAGGGCCGTCTTCCCCAGCATCGTGGGGCGCCCGCGCCACACTGGTGTCATGGTCGGGA---------------

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM5G859691_T01 (Zea mays), 3'ss of exon 7
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                              ||||  ||   || || |||    |  |  |    |   |  ||| ||| |     |||       || | |  ||  |     |       ||||||||| ||||| || ||||| |||||  |||  || |||||||||||||||||||| ||| | ||||| || || ||||| || ||||| ||||| ||||
-----------------------------------------------------------------------------gtaagccac-cggtttggtcacatctgttcttgagaatgcaa-ggttctcggtg-gctgctggggccctaacaacagcaccaacctcg-----gcagGCTGGGTTCGCTGGCGACGACGCCCCGAGGGTCGTCTTCCCCAGCATCGTGGGGCGCCCGCGCCACACTGGTGTCATGGTCGGGATGGGGCAGAAGGACG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G006765_T02 (Zea mays), 3'ss of exon 2
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                         || ||   | |  | || |    | | |   | ||     |||  | |    ||| |  ||| |  ||         ||||  |||||||||| || || |||||||| ||  ||||||||||||| ||||| || || || ||  | ||||| || |||||||| |||||||| ||||| || |
-----------------------------------------------------------------------------------------gtttg--tagttcttgttcacgctgcttcatgttcagtaacattgttctgttaattt--aatcacatcacaaactactggc--agGCTGGTTTTGCTGGTGACGATGCGCCAAGGGCTGTTTTCCCAAGCATTGTTGGACGCCCACGTCACACTGGCGTGATGGTTGGCATGGGGCAGAAGGATG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G126190_T02 (Zea mays), 3'ss of exon 2
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgt-ctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                  ||   | || ||  |  ||  | |||||  |||     |   | | | | |  |     ||        ||   |      |||| |   ||||||||||| || || |||||||| ||  ||||||||||||| ||||| || || || ||  | ||||| || |||||||| |||||||| ||||| || |
----------------------------------------------------------------------------------gtttgtagttcttgttcatgctggtacttcatattctgcaacctttttc-tgctaatttaatcacacgttttcttacaaactattgg---cagGCTGGTTTTGCTGGTGACGATGCGCCAAGGGCTGTTTTCCCAAGCATTGTCGGACGCCCCCGTCACACTGGTGTGATGGTTGGCATGGGGCAGAAGGATG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA03G30110.1 (Glycine max), 3'ss of exon 2
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                       || ||        | | |||||| |   |   |    | |||| |||    ||  ||||         || ||  ||| || |  ||   ||   || ||     ||| | ||    |||||||||| |||||||| ||||| ||  |||| || ||||||||||| |||||||| ||  | ||||| ||||||||||| || ||||| ||||| || |
-------------------------------------------------------tagcagagataa-tcgaatttgcgatcttt--tcccttaatcattcaacccaccttatat-------cccccttaatagtgtgttaatgttg---ttggg----atttgtgtgat--agGCTGGTTTTGCCGGAGATGATGCGCCGAGGGCGGTGTTCCCCAGCATTGTGGGGCGTCCACGTCACACGGGAGTGATGGTTGGGATGGGGCAGAAGGATG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: EFJ17401 (Selaginella moellendorffii), 3'ss of exon 1
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggc---gcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                                                      |  |  |   ||    |        | || | || | || ||    | ||     ||||||||| || |||||||||||||| ||| |||| || || |||||||| ||||| ||||| | |||||| || || ||||| || ||||| ||||| || |
---------------------------------------------------------------------------------------------------------------------gtgcgctcctggcgcggtttct----tgtggattttgatccggggggtgtgttcttgcagGCTGGATTTGCCGGAGACGATGCTCCCAGGGCCGTGTTTCCCAGCATTGTGGGCCGGCCCAAGCACACGGGCGTCATGGTTGGAATGGGGCAGAAGGATG

upper sequence: PP1S372_38V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: EFJ35077 (Selaginella moellendorffii), 3'ss of exon 1
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgc---gcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                                                                                                      |  |  |   ||   ||     |   | | |||     ||   ||| | |||   ||||||||| || |||||||||||||| ||| |||| || || |||||||| ||||| ||||| | |||||| || || ||||| || ||||| ||||| || |
---------------------------------------------------------------------------------------------------------------------gtgcgctcctggcgcgatttcttgtggattttgatccgggg---ggtgtgtttt-tgcagGCTGGATTTGCCGGAGACGATGCTCCCAGGGCCGTGTTTCCCAGCATTGTGGGCCGGCCCAAGCACACGGGCGTCATGGTTGGAATGGGGCAGAAGGATG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|208377765|gb|DC940877.1|DC940877
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
EST: gi|100409313|gb|BY991949.1|BY991949
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
EST: gi|100379823|gb|BY949962.1|BY949962
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
EST: gi|162156866|gb|FC333020.1|FC333020
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
EST: gi|100417941|gb|BY993260.1|BY993260
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
EST: gi|67703871|gb|BJ964104.1|BJ964104
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCG
EST: gi|100360875|gb|BY965946.1|BY965946
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
EST: gi|100380171|gb|BY968883.1|BY968883
EST:     GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAG                         GCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG
genomic: GTGAGGAAGTCCAGCCTTTGGTGTGCGACAATGGCTCTGGAATGGTTAAGgtatgcatgc ... tttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTG


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG
                                         tttattt  CT-rich tract
                    aaaatctgaaatta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgcatgcttaccgcatatacgactgtaattctcccatgcaaatcgagcatgctaacaatcgcgccttgcatttgctaaggtacattgtctgatcaatcatagtactatatacatatcggtcctctgcataatgcgcaaaatctgaaattaggtctggtttatttggcgcagGCTGGTTTCGCCGGAGACGATGCCCCCCGGGCTGTTTTCCCCAGCATCGTGGGGCGGCCGAGGCACACCGGAGTGATGGTGGGCATGGGACAGAAAGACG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG