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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgatggtttctttgatcatgtgtacaatgtaaaacttggcgttatttttgtttgtcctccctgcaccgcattgtctgccgttttccttgacctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG

Basic information

species Physcomitrella patens
transcript PP1S25_89V6.2
intron # 2
splice site 3'
intron type U12 (go to U12 genes for details)

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S25_89V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA10G33550.2 (Glycine max), 3'ss of exon 2
--------------------------------------------------tgatggtttctttgatcatg----tgtacaatgtaaaact-tggcgtt---atttttgtttgtcc-----------tccctgcaccgcattgtctgccgttttccttg-accta-----------ttacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG
                                                  |  | |||| || | |||      | |  |||||||| ||   |  ||   |||||| ||| |             | |    |     ||   | | |  ||||  | || |            || ||| || || || || || |||  ||| ||||||||||| ||||||||||| |||||||| |||||||||   || |||||||||||||||||| | ||||||
gtgagtcagttattagcaactatacatatccagttatcgatatcaaatgttttttgttttttagctcaaaatgttttttaatgtaaagctgcagttttttaatttttattttttggggattgggggttctgagaattttttaattactgaattccacgcacgtggttattatgacttgcagGGCGAGTGAGAAATGCCAGAAGGAGAAGAGAAAGACAATCAATGGAGACGATTTACTATGGGCCATGGCAACTTTAGGGTTTGAAGACTACATTGAGCCG

upper sequence: PP1S25_89V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA20G34050.1 (Glycine max), 3'ss of exon 1
---------------------------------------------------------------------------tgatggtttct-ttgatcatgtgtacaatgtaaaacttggcgttatttttgtttgtcctccctgcaccgcattgtctgc-cgttttccttgacctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG
                                                                            |||| || |  ||  | || | ||   | |     |||  | | | |  |  | |  |   | |          |  |  ||||   | | | |    ||| || || || || || |||  ||| ||||||||||| ||||||||||| ||||| || |||||||||  ||| ||||| |||||||||||| | ||||||
gtcagttagcaactatacatatccatatcaaatgttttgtttttatttagctcaaaatgtctttttttttttaatggatgattgcagttttttatttttattttttggggattgagggtttctgagaatttgtttatttctgaattccacccacgtgtttatttcggcgtg---cagGGCGAGTGAGAAATGCCAGAAGGAGAAGAGAAAGACAATCAATGGAGACGATTTGCTATGGGCCATGGCCACTTTAGGATTTGAAGACTACATAGAGCCG

upper sequence: PP1S25_89V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA19G36220.1 (Glycine max), 3'ss of exon 2
-----tgatggtttctttgatcatgtgtacaatgtaaaacttggcgttatttttgtttgtcctccctgcaccgcattgtctgccgttttccttgacctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG
     ||    |||||||  |  |   ||  ||  |     |  |||| ||| | ||  |    | | |   || ||   | |   |    || | |   |  || ||    || ||||||||| |||||||||||||||| || || || |||||||| || |||||||||  |||  | || ||||| || ||  |||| || 
gttagtgtctttttcttttctttttcttatgattcattctcttccgttttttctcttattatatcatcccgtgctttaatttcgaatccgtttaatcg--tgtagGGCCTCTGATAAGTGTCAGAGGGAAAAGAGAAAGACTATTAACGGTGATGATTTGCTCTGGGCCATGGCCACTCTTGGTTTTGAGGATTATATCGATCCT

upper sequence: PP1S25_89V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: AT3G53340.1 (Arabidopsis thaliana), 3'ss of exon 2
-----------------------------------------------tgatggtttc----tttgatcatgtgtacaatgtaaaact----tggcgttatttttgtttgtcctccctgca------ccgcattg--tctgccgttttccttgacctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG
                                               || |||||      | | |     | | |  ||| ||  |    ||  ||||| ||  || |        |||      |    | |  | ||   | ||  ||  ||| |||  | ||||| || ||||| ||  | || || || ||||| ||||||||||||||||| |||||||| |||  ||| ||||| || ||||| ||| |||| || 
gtaatttctttctctactttctcaaaaatgttacctttttgttgctttggtggttatagcgtgtaaagccctcttctttgttaagttagattgatgttatattcattgggttgttaagcaaagtcactatgtcgattttggaatattgttttgcctttta--gGGCTAGTGATAAGTGCCAAAGAGAGAAAAGGAAGACCATCAATGGAGATGATTTGCTTTGGGCTATGGCCACTTTAGGATTCGAAGATTACATCGATCCC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67697971|gb|BJ958204.1|BJ958204
EST:     GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
EST: gi|162273472|gb|FC449617.1|FC449617
EST:     GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
EST: gi|208354191|gb|DC957411.1|DC957411
EST:     GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
EST: gi|208361426|gb|DC935113.1|DC935113
EST:     GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
EST: gi|162273471|gb|FC449616.1|FC449616
EST:     CAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: CAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
EST: gi|37828975|gb|BJ586987.1|BJ586987
EST:     TCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: TCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
EST: gi|208387454|gb|DC949239.1|DC949239
EST:     GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGA                         AGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA
genomic: GAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 31 (upstream exon), 31 (downstream exon)
Score: 8

TCATGAAGAAGGCGTTGCCTGCTAATGCCAAGATTGCCAAAGATGCGAAGGAGACAGTTCAAGAGTGTGTTTCCGAATTCATCAGTTTCATCACGAGCGAgtatccattt ... cctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttttgtttgtcctccctgcaccgcattgtctgccgttttccttgacctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG
                                     ttccttgac  putative branch site 
                                               tattaca  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgatggtttctttgatcatgtgtacaatgtaaaacttggcgttatttttgtttgtcctccctgcaccgcattgtctgccgttttccttgacctattacagAGCTAGCGACAAGTGTCAGCGGGAAAAGAGAAAGACGATCAATGGAGATGATTTACTTTGGGCCATGAGCACATTAGGGTTTGAAGACTACGTCGAGCCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA