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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tatacatagtatttcacataatcaggtgttgagtagacttttaaagagtatatgttgttactgcatggcatttaatcatcttgaatggaatgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGGAAGGGCAGTGTCAGGAG

Basic information

species Physcomitrella patens
transcript PP1S479_10V6.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S479_10V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: Vv17s0053g00620.t01 (Vitis vinifera), 3'ss of exon 1
--tatacatagtatttcacataatcaggtgttgagtagacttttaaagagt-atatgttgttactgcatg-gcatttaatcatcttgaatggaatgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGGAAGGGCAGTGTCAGGAG
  | |||||   ||||  | |    |  |  | || |||| |  |  |  |  |||  |   | | |||  | ||     ||  ||        ||    ||||||| |||| |||||||| |||||||||||| ||||||| ||||| ||||| ||||| ||  | || ||
cttttacattcaattt-gcctgtatatttcattagaagacctggattgccttgtatagtagaaattcatttgtatccttacactttcctctctttgt---gcagATGAATAGGGAGAAGCTTATGAAGATGGCTGGTGCAGTTCGCACCGGTGGAAAGGGTAGCATGAGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67571898|gb|BJ944722.1|BJ944722
EST:     CCTCNGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: -GTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162274367|gb|FC450394.1|FC450394
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162244329|gb|FC421007.1|FC421007
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18342856|gb|BJ174892.1|BJ174892
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18353276|gb|BJ185331.1|BJ185331
EST:     AGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: AGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162240484|gb|FC414373.1|FC414373
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162224828|gb|FC400828.1|FC400828
EST:     CCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: CCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18337154|gb|BJ169178.1|BJ169178
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162279458|gb|FC455574.1|FC455574
EST:     CGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: CGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162224827|gb|FC400827.1|FC400827
EST:     CCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: CCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|37833743|gb|BJ591755.1|BJ591755
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCNTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18328912|gb|BJ160918.1|BJ160918
EST:     GGCCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGATGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162229541|gb|FC405875.1|FC405875
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67507846|gb|BJ940722.1|BJ940722
EST:     TCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: TCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18328944|gb|BJ160950.1|BJ160950
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67575474|gb|BJ948298.1|BJ948298
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67698944|gb|BJ959177.1|BJ959177
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162235488|gb|FC411311.1|FC411311
EST:     GGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162235489|gb|FC411312.1|FC411312
EST:     GGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67576767|gb|BJ949591.1|BJ949591
EST:     GTCAGCTCGGCATCCCTCGCCGAACCAGTGTTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GTCAGCTCGGCATCCCTCGCCGAACCAGTG-TCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67579122|gb|BJ951273.1|BJ951273
EST:     CCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: CCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18356803|gb|BJ188862.1|BJ188862
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162222208|gb|FC397644.1|FC397644
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18357996|gb|BJ190055.1|BJ190055
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67695264|gb|BJ955497.1|BJ955497
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162227817|gb|FC403967.1|FC403967
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162274368|gb|FC450395.1|FC450395
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162239783|gb|FC417182.1|FC417182
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162274496|gb|FC450731.1|FC450731
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67699627|gb|BJ959860.1|BJ959860
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|67576136|gb|BJ948960.1|BJ948960
EST:     TCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: TCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162279459|gb|FC455575.1|FC455575
EST:     CGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: CGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162241574|gb|FC414696.1|FC414696
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCTGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTATTGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|18353616|gb|BJ185671.1|BJ185671
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162159448|gb|FC336237.1|FC336237
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|37822000|gb|BJ580066.1|BJ580066
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162229542|gb|FC405876.1|FC405876
EST:     GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GGTCAGCTCGGCATCCCTCGCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
EST: gi|162203658|gb|FC379144.1|FC379144
EST:     GCCGAACCAGTGTCGCCGACGGCCTTCCAAG                         ATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG
genomic: GCCGAACCAGTGTCGCCGACGGCCTTCCAAGgtacgcgtct ... tgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGG


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gagtatatgttgttactgcatggcatttaatcatcttgaatggaatgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGGAAGGGCAGTGTCAGGAG
                        atttaat  putative branch site (score: 4)
                        atttaatcat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tatacatagtatttcacataatcaggtgttgagtagacttttaaagagtatatgttgttactgcatggcatttaatcatcttgaatggaatgccgagcagATGGATAGAGAGAAGCTAATGAAGATGGCTAGTGCAGTGCGCACTGGTGGGAAGGGCAGTGTCAGGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG