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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagaacgaagatgagaagatagtttcttctgtctttctatactgaatttgaatttgaaccactcaaatttgacgagaatggctgttccttcttttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCCGATCATGGCAACGCCGAGGATATGGTGAAGAGGAACAAATCCGGCCAGC

Basic information

species Oryza sativa
transcript LOC_Os05g40420.1
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g40420.1 (Oryza sativa), 3'ss of exon 8
lower sequence: GLYMA09G40690.1 (Glycine max), 3'ss of exon 7
gtgagaacgaagatgagaagatagtttcttctgtctttctatactgaatttgaatttgaaccactcaaatttgacgagaatggctgttccttcttttgtg-----atagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCCGATCATGGCAACGCCGAGGATATGGTGAAGAGGAACAAATCCGGCCAGC
          |  | || |  || |    |          | |    |  ||||    ||  |   |    |       ||   |||   ||    ||||       |||||||||| ||||| || |||||||||||||| || ||| | |||||||| || ||||| || || ||||||||||| |||||| | ||||| ||  | |
---------caattaagcaattactgctataaagaaacaaacaacagagatgaaggctaaatagctactcattgtattgattaatgtatatttacatgtgtaattgcagATGATTCTTGATGCAATAGAGCAAGTGGGTGGAATTTATGTTGTCACCGCTGACCATGGGAATGCAGAGGATATGGTCAAGAGGGATAAATCTGGAAAAC

upper sequence: LOC_Os05g40420.1 (Oryza sativa), 3'ss of exon 8
lower sequence: Vv08s0056g00200.t01 (Vitis vinifera), 3'ss of exon 7
gtgagaacgaagatgagaagatagtttcttctgtctttctatactgaatttgaatttgaaccactcaaatttgacgagaatggctgttcct------tcttttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCCGATCATGGCAACGCCGAGGATATGGTGAAGAGGAACAAATCCGGCCAGC
         ||||  |  | | |||||     || | |  | |   ||  ||    | ||    | | | |   ||| | | |||| |  |      | | |  |   |||| || || ||||| || |||||||||||||| || ||| | ||||| || |||||||| || || ||||| |||||||||||||| || || || ||||
---------aagagaaacatagagttttggtggtgtatggacagaaaaagtgctactaaatt-tttataatgagcgaaattagctgattataaaaactgtatgattgcagATCATCCTTGATGCAATAGAGCAAGTGGGTGGAATATATGTTGTCACTGCAGATCATGGAAATGCTGAGGACATGGTGAAGAGGAATAAGTCTGGTCAGC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88839805|gb|CI017570.1|CI017570
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|86592517|gb|CI253198.1|CI253198
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATCTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89168322|gb|CI109526.1|CI109526
EST:     AAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: AAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|87022092|gb|CI259164.1|CI259164
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|87026872|gb|CI259525.1|CI259525
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|86478225|gb|CI120858.1|CI120858
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|86251274|gb|CI055407.1|CI055407
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|88837678|gb|CI079038.1|CI079038
EST:     AGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: AGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|88847922|gb|CI006108.1|CI006108
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|8527713|gb|AU092528.1|AU092528
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATNCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|55409364|gb|CV721740.1|CV721740
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|3763744|gb|AU030496.1|AU030496
EST:     ACATTGAGCCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGNATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|38475605|gb|CF959738.1|CF959738
EST:     ACATTGAGGCCACAGTGGGTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89198567|gb|CI016652.1|CI016652
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|33812546|gb|CF332161.1|CF332161
EST:     CATTGAGGCNCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: CATTGAGGC-CACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|58592226|gb|CF990534.1|CF990534
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|86907723|gb|CI298640.1|CI298640
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|218489939|gb|FG958028.1|FG958028
EST:     ACATTGAGGCCACAGTGGTTGCATGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|33812548|gb|CF332162.1|CF332162
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|87024582|gb|CI259297.1|CI259297
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89192294|gb|CI049755.1|CI049755
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89175858|gb|CI042879.1|CI042879
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|29684030|gb|CB680305.1|CB680305
EST:     ACATTGAGGCC-CCGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|86261542|gb|CI017284.1|CI017284
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|58589221|gb|CF987529.1|CF987529
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89195422|gb|CI051535.1|CI051535
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89194679|gb|CI117116.1|CI117116
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|88215087|gb|CI214936.1|CI214936
EST:     GGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: GGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|88991606|gb|CI019784.1|CI019784
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|88990572|gb|CI107959.1|CI107959
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|88216968|gb|CI216672.1|CI216672
EST:     GGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: GGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|86420372|gb|CI056361.1|CI056361
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
EST: gi|89175355|gb|CI042376.1|CI042376
EST:     ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAG                         ATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC
genomic: ACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 70 (upstream exon), 74 (downstream exon)
Score: 42

GTCCGCGTTAACCTCCCAAATGGTGATATGGTGGGTCACACTGGTGACATTGAGGCCACAGTGGTTGCTTGCAAAGCTGCTGATGAGGCTGTCAAGgtgagaacga ... tttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCCGATCATGGCAACGCCGAGGATATGGTGAAGAGGAACAAATCCGGCCAGC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttgaatttgaaccactcaaatttgacgagaatggctgttccttcttttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCCGATCATGGCAACGCCGAGGATATGGTGAAGAGGAACAAATCCGGCCAGC
                   atttgac  putative branch site (score: 42)
                                  ctgttccttcttttgt  putative PPT
               tcaaattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagaacgaagatgagaagatagtttcttctgtctttctatactgaatttgaatttgaaccactcaaatttgacgagaatggctgttccttcttttgtgatagATGATTCTGGATGCCATTGAGCAAGTGGGTGGCATCTATTTGGTCACCGCCGATCATGGCAACGCCGAGGATATGGTGAAGAGGAACAAATCCGGCCAGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGGA