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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...atagttgacagaccacacttatttttgaaatcaagttgtcaccctttgacatttctgtagttctatatgatgaccgcagttctatgattttattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGCAAGCATTTATGTTCTGAACTAGAGCAGATACATGATGCTCTGTGTGGTT

Basic information

species Oryza sativa
transcript LOC_Os05g33810.1
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g33810.1 (Oryza sativa), 3'ss of exon 8
lower sequence: GRMZM2G109354_T01 (Zea mays), 3'ss of exon 8
atagttgaca-gaccacacttatttttgaaatcaagttgtcaccctttgacatttctgtagttctatatgatgaccgcagttctatga--ttttattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGCAAGCATTTATGTTCTGAACTAGAGCAGATACATGATGCTCTGTGTGGTT
 | ||| ||| ||  |   |    | | |    ||    | |   ||  |   |   || |  || | | |||     | || ||||   | || |||||||||| ||||||||||||||| ||||||||||||||||||||||| |||| ||||| || || |||||||||||||||  ||||||||||||||| |||||||
-tggtttacatgatgatgatccactgtcagtctaataaattatgtttgcagtatatggttgaact-tttaatgtttaga-ttgtatggattctttttttgcagGTTGAAAACATGTTTGGGTTATCAAATTTTGTTCCTATCCTTTTTGGAAGCAAACAGTTGTGTTCTGAACTAGAGAGGATACATGATGCTCTTTGTGGTT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|66925673|gb|CX112521.1|CX112521
EST:     TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAA                         GTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
genomic: TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
EST: gi|29682373|gb|CB678648.1|CB678648
EST:     TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAA                         GTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
genomic: TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
EST: gi|218482951|gb|FG948113.1|FG948113
EST:     TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAA                         GTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
genomic: TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
EST: gi|29619149|gb|CB624161.1|CB624161
EST:     TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAA                         GTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
genomic: TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
EST: gi|313084053|gb|HO213836.1|HO213836
EST:     TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAA                         GTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
genomic: TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
EST: gi|218498191|gb|FG955475.1|FG955475
EST:     TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAA                         GTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC
genomic: TAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 72 (upstream exon), 57 (downstream exon)
Score: 30

TATTGGAAGTGGGGAACATATACTTGATTCTCAACATGAAATATTCCGGATAAATATTACCACATCAAAGCTGGATACTCATGGACCCGCATTTGTGGAAgtaagtatct ... tattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGCAAGCATTTATGTTCTGAACTAGAGCAGATACATGATGCTCTGTGTGGTT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttgacatttctgtagttctatatgatgaccgcagttctatgattttattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGCAAGCATTTATGTTCTGAACTAGAGCAGATACATGATGCTCTGTGTGGTT
 ctttgac  putative branch site (score: 30)
                                          ttttatttt  putative PPT
                                  ttctatgattttattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
atagttgacagaccacacttatttttgaaatcaagttgtcaccctttgacatttctgtagttctatatgatgaccgcagttctatgattttattttgcagGTGGAAAACATGTTTGGGCTATCAAATTTTGTTCCTATCCTTGTTGGTAGCAAGCATTTATGTTCTGAACTAGAGCAGATACATGATGCTCTGTGTGGTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGAT
- - - - - - - - - - - - - -aaatcaa
- - - - - - - - - - - - - - - - - - - - - - - - - - ttctgta
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - atgatga