ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaaatgaaatattactacttgtcacacattataatttgcattttttgggatcattttttgtgaggttttctgttgtttagttcgaactaatatctccttggctcataatttatttctgcaaaacgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG

Basic information

species	Oryza sativa
transcript	LOC_Os03g38590.1
intron #	8
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os03g38590.1 (Oryza sativa), 3'ss of exon 8
lower sequence: GRMZM2G124918_T01 (Zea mays), 3'ss of exon 8

gtaaatgaaatattactacttgtcacacattataatttgcattttttgggatcatttt--ttgtgaggttttctgttgtttagttcgaactaatatctccttggctcataatttatttctgcaaaacgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
                ||  ||  | | |||| |   |    |   |  ||  |||    || | |  | |    ||| | || ||    ||| ||     |     |||   |   | ||         | ||||||| ||||| ||||| |||||||||||||||||||| |||||||||
---------------gtaaatgcaatagattaaatcgtttcctccctatgaatattacagttattatatctctacttggtgagctccccgtaaaattattatccactataccgtgcatgtgt--------ttacagGAACGAGAGCGCTCTTAAATTTGGATGGTTTTTCCTATTCTACTTG

upper sequence: LOC_Os03g38590.1 (Oryza sativa), 3'ss of exon 8
lower sequence: GRMZM2G011078_T01 (Zea mays), 3'ss of exon 8

gtaaatgaaatattactacttgtcacacattataatttgcattttttgggatcattttttgtgaggttttctgttgtttagttcgaactaatatctccttggctcataa-tttatttctgcaaaacgtat-cacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
                                          |   || ||| ||        | ||  | |  |   ||     || ||| || ||  |  |  |||| ||| | ||||||| || | |   |||||| ||||| ||||| || || |||||||||||||| |||||||||
-----------------------------------------gtaaatgcgatgatacccac--aagtcgtttcctcccta----caaataacatttcactctcatataagtttctgtctgcaacacatgtgtgcagGAACGAGAGCGCTCTCAAGTTCGGATGGTTTTTCCTCTTCTACTTG

upper sequence: LOC_Os03g38590.1 (Oryza sativa), 3'ss of exon 8
lower sequence: GLYMA07G31090.1 (Glycine max), 3'ss of exon 8

gtaaatgaaatattactacttgtcacacattataatttgcattttttgggatcattttttgtgaggttttctgttgtttagttcgaactaatatctccttggctcataatttatttctgcaaaacgtatca-cagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
                            |||  |    |||||  |  | |  ||        | |  | |   | || |  |     | || |  ||||       |  ||   | |||    | | |   ||||||||| |||||| ||||||||||||||||||||||||| ||  ||
----------------------------attgaa----gcattcatgagaacaataggcaaatatgcatgcatattttcatctt---tttattttgccttactgttgatcttgaatatgctgctctttttttcagGAATGAAAGTGCTTTGAAATTTGGATGGTTTTTCCTGTTTTATCTG

upper sequence: LOC_Os03g38590.1 (Oryza sativa), 3'ss of exon 8
lower sequence: GLYMA13G25460.1 (Glycine max), 3'ss of exon 7

gtaaatgaaatattactacttgtcacacattataatttgcattttttgggatcattt--tttgtgaggttttctgttgtttagttcgaactaatatctc--cttggctcataat-ttatttctgcaaaacgtatc-acagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
                                        |  | | |   ||| ||  | | | |  |||  | ||| | ||    ||  || |  |     |  ||  | || ||   | |||    | | |   ||||||||| |||||| |||||||||||||||||||| |||| ||  ||
----------------------------------------acctctggtactcaattaatatataatatttatttttgctgagcaaaaaaaaaaaatttggtcttactgttgatcttgaatatgctgttcttttttgcagGAATGAAAGTGCTTTGAAATTTGGATGGTTTTTCATGTTTTATCTG

upper sequence: LOC_Os03g38590.1 (Oryza sativa), 3'ss of exon 8
lower sequence: Vv00s0301g00030.t01 (Vitis vinifera), 3'ss of exon 8

gtaaatgaaatattactacttgtcacacattataatttgcattttttgggatcattttttgtgaggttttctgttgtttagttcgaactaatatctccttggctcataatttatttctgcaaaacgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
                               |  ||    || | |  |  |   | | |||  |  | | |||| | | |||  | || | |  |   ||     | ||      || ||| |  | | |||||||||| |||||| |||| ||||||||||||||| |||| || |||
-----------------------------ccaccatccatatgtgt--gtgtgcatgtatgtttgtatgtgtgtt-tatggttttacctgacaattttctgttatctgatca--cactacaacatttcttacagGAATGAAAGTGCTTTGAAGTTTGGATGGTTTTTCATGTTTTATTTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|29662769|gb|CB659044.1|CB659044

EST:     CGACCACTTTACCGTGCCATGAG                         GAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCGGTTCTACTGG
genomic: CGACCACTTTACCGTGCCATGAGgtaaatgaaa ... cgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG

EST: gi|25799630|gb|CA755591.1|CA755591

EST:     TGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAG                         GAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
genomic: TGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAGgtaaatgaaa ... cgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG

EST: gi|29662682|gb|CB658957.1|CB658957

EST:     TGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAG                         GAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
genomic: TGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAGgtaaatgaaa ... cgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG

EST: gi|29662718|gb|CB658993.1|CB658993

EST:     TGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAG                         GAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG
genomic: TGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAGgtaaatgaaa ... cgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 71 (upstream exon), 34 (downstream exon)
Score: 2

GTCCCAAGATCTGGTTTCTTGCTGTCATCTATTTCATTCTTGGATGCCCAGGTGCCTACTATCTGTGGTACCGACCACTTTACCGTGCCATGAGgtaaatgaaa ... cgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG

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atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

agttcgaactaatatctccttggctcataatttatttctgcaaaacgtatcacagGAATGAGAGTGCTCTGAAATTTGGATGGTTTTTCCTGTTCTACTTG