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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgaggtggacttgcgatctggtgcatttggttgggtgatcattaatgctgcttatttatgatgattctggagcatctggctggatgattaatgctgcttatttataatgattttttttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC

Basic information

species Oryza sativa
transcript LOC_Os06g02380.2
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os06g02380.2 (Oryza sativa), 3'ss of exon 7
lower sequence: GRMZM2G095252_T02 (Zea mays), 3'ss of exon 1
gtgaggtggacttgcgatctggtgcatttggttgggtgatcattaatgctgcttatttatgatgattctggagcatctggctggatgattaatgctgcttatttataatgattttttttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC
                                               |    |||   |      |||| |||||||    || || ||  ||||| ||| |||||||  ||| ||| | |||||||||||||||||||| ||||||||||||||||||||||||||||| |||||||||||  | ||||| || |||||||| |||||||  |||||||| |||||
-----------------------------------------------gtcagttaa--acttccgttctagagcatccttatg-atcatgtatgctccttgtttataagcattctttgtttctgcagCAACTGTAATCAGAGATGAAGTTGGGCTGTCACTTGACAAGGCTGACAAATCAGTCCTTGGAACAGCAGCAAAGGTTGTCCTTACTAAAGAGTCGACAAC

upper sequence: LOC_Os06g02380.2 (Oryza sativa), 3'ss of exon 7
lower sequence: GRMZM2G083716_T04 (Zea mays), 3'ss of exon 6
gtgaggtggacttgcgatctggtgcatttggttgggtgatcattaatgctgcttatttatgatgattctggagcatctggctggatgattaatgctgcttatttataatgattttttttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC
                                               |     ||   |     |||||  ||| ||    | ||| ||  ||||  |||||  ||||  ||  ||| | |||||||||||||||||||| ||||||||||||||||||||||||||||| |||||||||||| | ||||| ||||||||||| |||||||  |||||||| |||||
-----------------------------------------------gtcagctaa--acttccattctaaagcttc-attttgatcatgtatgcaccttatccataaacatactttgtttctgcagCAACTGTAATCAGAGATGAAGTTGGGCTGTCACTTGACAAGGCCGACAAATCAGTTCTTGGAACAGCTGCAAAGGTTGTCCTTACGAAAGAGTCGACAAC

upper sequence: LOC_Os06g02380.2 (Oryza sativa), 3'ss of exon 7
lower sequence: GRMZM2G065023_T01 (Zea mays), 3'ss of exon 2
gtgaggtggacttgcgatctggtgcatttggttgggtgatcattaatgctgcttatttatgatgattctggagcatctggctggatgattaatgctgcttatttataatgattttttttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC
                                               |     ||   |     |||||  ||| ||    | ||| ||  ||||  |||||  ||||  ||  ||| | |||||||||||||||||||| ||||||||||||||||||||||||||||| |||||||||||| | ||||| ||||||||||| |||||||  |||| ||| |||||
-----------------------------------------------gtcagctaa--acttccattctaaagcttc-attttgatcatgtatgcaccttatccataaacatactttgtttctgcagCAACTGTAATCAGAGATGAAGTTGGGCTGTCACTTGACAAGGCCGACAAATCAGTTCTTGGAACAGCTGCAAAGGTTGTCCTTACGAAAGGGTCGACAAC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|7212768|gb|D46006.2|D46006
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|6934900|gb|AU082437.1|AU082437
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|87011216|gb|CI287893.1|CI287893
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|29616041|gb|CB621053.1|CB621053
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|88325601|gb|CI648035.1|CI648035
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|218493375|gb|FG949729.1|FG949729
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|58687616|gb|CK076303.1|CK076303
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|86906130|gb|CI292661.1|CI292661
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
EST: gi|58690567|gb|CK079254.1|CK079254
EST:     TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAG                         CAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT
genomic: TGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 75 (upstream exon), 75 (downstream exon)
Score: 217

AACAAGCTTAGAGGGTCATTGAAAATTGCCGCTATCAAAGCCCCTGGTTTTGGTGAGCGCAAGACCCAGTACTTGGATGATATTGCCATTCTAACTGGAGgtgaggtgga ... ttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gcatctggctggatgattaatgctgcttatttataatgattttttttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC
              gattaat  putative branch site (score: 217)
                                       ttttttttctctgc  putative PPT
                          ttatttataatgattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgaggtggacttgcgatctggtgcatttggttgggtgatcattaatgctgcttatttatgatgattctggagcatctggctggatgattaatgctgcttatttataatgattttttttctctgcagCAACTGTAATCAGGGATGAAGTTGGGCTGTCACTTGACAAGGCAGACAAATCAGTTTTAGGAACGGCTGCAAAGGTGGTCCTTAACAAAGAGTCAACAAC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAAG