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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgacaccgatatgtttcttttgaattcaactgttgtgtacccaagagctgaaaatataaataccctattgagtgcataacctggctgaactctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT

Basic information

species Oryza sativa
transcript LOC_Os02g35470.4
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os02g35470.3 (Oryza sativa), 3'ss of exon 7
lower sequence: GRMZM2G054109_T01 (Zea mays), 3'ss of exon 7
tgacaccgatatgtttcttttgaattcaactgttgtgtacccaagagctgaaaatataaa-taccctattga---gtgcataacctggctgaactcta-ttgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT
     |    || | |    | |||  ||  | | || ||||||   ||  |   |   | |   ||| |||   ||  |||     |||    |  |  |||||  |||||| |||| |||||| ||||||||||||||||| |||||||| ||||||||||||||||||||||||||| ||||||||||||||||||||||||
-----ctaggatctattagctaaatcaaaagggtttggacccaaatcctctatccaaggagtggtctactgacatgtttatatttctgctcgttttaaactgcagGGCTATGCTGATGCATTGGTAAAGAAAGCATATGAGGACTGGATGCAAGTTGTAGAATATGATGGCAAGGCACTCTTGAGCTTCAAGCAGAAAAAGAAAT

upper sequence: LOC_Os02g35470.3 (Oryza sativa), 3'ss of exon 7
lower sequence: GRMZM2G113453_T01 (Zea mays), 3'ss of exon 7
---tgacaccgatatgtttcttttgaattcaactgttgtgtacccaagagctgaaaatataaataccctattgagtgcataacctggctgaactcta-ttgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT
    |   |    || | |    | |||||||        | | | |    |  ||  |    |   ||     ||   ||     ||| | ||  |   |||| ||||||| |||| |||||| ||||||||||||||||| |||||||| ||||||||||||||||||||||||||| ||||||||||||||||||||||| 
ggaagtttctaggatctattagctaaattcaaagagcaaattctcta----ttcaaggagtggtctactgagatgtttgtatttgtgctcatcttaaaccgcagGTCTATGCTGATGCATTGGTAAAGAAAGCATATGAGGACTGGATGCAAGTTGTAGAATATGATGGCAAGGCACTCTTGAGCTTCAAGCAGAAAAAGAAAA

upper sequence: LOC_Os02g35470.3 (Oryza sativa), 3'ss of exon 7
lower sequence: GLYMA15G07330.2 (Glycine max), 3'ss of exon 7
tgacaccgatatgtttcttttgaattcaactgttgtgtacccaagagctgaaaatataaataccctattgagtgcataacctg-gctgaactctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT
     ||  | |     | |||   |    |    | | | | | |  |     || |  |     | |   || | ||  || |     |  | ||| || | ||||  ||   |||||| ||||| ||||||||| |||||||||||||| | || ||||||||| || | || || |  | ||| ||||| ||||  |
-tttgccagttttaaaatgttgctctggcataggatatccaccatatttttttttaaacttgtgtaaatttctgaaaaatgtgagtctctcattcttgtagGTTTATGTGGACACATTGGTGAAGAAGGCATATGAGAATTGGATGCATGTTATTGAGTATGATGGCGAGTCTCTGCTAAATTACAATCAGAATAAGACTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|29667009|gb|CB663284.1|CB663284
EST:     TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
genomic: TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
EST: gi|58607528|gb|CK035561.1|CK035561
EST:     TCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTGAGAAAGCATATGAGGATTGGATGCATGTT
genomic: TCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
EST: gi|29666753|gb|CB663028.1|CB663028
EST:     TGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
genomic: TGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
EST: gi|84117303|gb|CD151070.1|CD151070
EST:     TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
genomic: TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
EST: gi|29668182|gb|CB664457.1|CB664457
EST:     TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
genomic: TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
EST: gi|87007707|gb|CI287551.1|CI287551
EST:     TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
genomic: TTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
EST: gi|3761547|gb|C98795.1|C98795
EST:     GCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAG                         ATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT
genomic: GCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 57 (upstream exon), 71 (downstream exon)
Score: 47

AAGAAATGTCGGTGCAATATTCAATAACATCTATGAGTTCACTGGTTTGATTGCTGACGACCAGTTCATTTCAGCAGAAAATCTCACTGACAACCAGAAGgttggcatct ... tctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agctgaaaatataaataccctattgagtgcataacctggctgaactctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT
                            gcataac  putative branch site (score: 47)
                                            ctctatt  putative PPT
     aaaatataaata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgacaccgatatgtttcttttgaattcaactgttgtgtacccaagagctgaaaatataaataccctattgagtgcataacctggctgaactctattgcagATCTATGCAGATGGATTGGTTAAGAAAGCATATGAGGATTGGATGCATGTTGTAGAATATGATGGCAAGGCACTCCTGAGCTTCAAGCAGAAAAAGAAAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA
- - - - - -tgtttct
- - - - - - - - - - - - - - -ctgttgt
- - - - - - - - - - - - - - - - - - - - - - -agctgaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gtgcata
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -cctggct