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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagttaactgtcatgtctgattctttggtattcttctgcaggagtcattttgtaacatattaaataatgtgtattagacttgagctaatttgcttgctgttgctactactttccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG

Basic information

species Oryza sativa
transcript LOC_Os08g42560.1
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os08g42560.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM5G821551_T03 (Zea mays), 3'ss of exon 6
gtgagttaactgtcatgtctgattctttggtattcttctgcaggagtcattttgtaacatattaaataatgtgtattagacttgagctaatttgcttgctgttgctactactttccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG
               || |  |||   | |  ||   ||  |    |||  ||| ||  | |||  | ||| | |||| || || ||| || || ||| ||       || ||  |||||||||||||||| ||||| ||||| ||||||||||||||||| ||||||||||| ||||||||||||||  | ||||||||||||
-------------agtgccctattacatagattttccctttatccttcaaattgaaat-tgttat-ttatg-gcatta-acatgtgcttatatgtttggtga------tatttatcattgcagAAACTTGTCATAGTTCCCTCGAAGAAAGACCTGGGGCTGGCTTTTAAGGGCAACCAAAAGATGGTCCTGGAAGCATTGGAG

upper sequence: LOC_Os08g42560.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G041797_T01 (Zea mays), 3'ss of exon 6
--------------------------------------------gtgagttaactgtcatgtctgattctt----tggtatt-----------cttctgcaggagtcattttgtaacatattaaataatgtgtatta--gacttgagctaatttgcttgctgttgctactactttccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG
                                                ||||| |  ||||  || || ||     ||||              |  |  |    |  ||||  |  | | | || | |   ||||   | | | | |   |     |    |||||  || ||   ||||||||||||||| ||||| ||||| ||||||||||||||||| ||||||||||| ||||||||||||||  ||||||| ||||||
gtgagatatcatatccagtttctgcagtagctatatcttagttttcatgttaaata-catgcatggtttttaaagcggtagcgagacaaggcgttgttatatagatttttttccattaaaatgaaaagttgttatttatggcattaacatgtgcttatatgtttgctgatatttattattgcagAAACTTGTCATAGTTCCCTCGAAGAAAGACCTGGGGCTGGCTTTTAAGGGCAACCAAAAGATGGTCCTTGAAGCTTTGGAG

upper sequence: LOC_Os08g42560.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GLYMA08G47890.1 (Glycine max), 3'ss of exon 4
-----gtgagttaactgtcatgtctgattctttggtattcttctgcaggagtcattttgtaacatattaaataatgtgtattagacttgagctaatttgcttgctgttg-----ctactactttccattg---cagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG
     ||    |   |   ||||    || |||   | | | |   |   |||  ||     ||  || |       | |  |  || ||    |||||        ||     ||  | || | |||||   |||||| | ||||||   ||   ||||||||| ||||| || || || ||||||||||| | |||||||||||||||| | |||
gtactgtttaatgcttaagatgttgatttgtttatgaatatacatgacttgtcccttcccttcaacttcatgctcaagcacaactctagaaaatatttggacatcactgattgacttgttctgtacattgtttcagAAATTGGTTATAACTCCAGTAAAGAAAGAGCTGGGTCTGGCATTCAAGGGGAACCAGAGGATGGTGGTTGAAGCACTTGAG

upper sequence: LOC_Os08g42560.1 (Oryza sativa), 3'ss of exon 6
lower sequence: Vv01s0150g00150.t01 (Vitis vinifera), 3'ss of exon 6
gtgagttaactgtcatgtctgattctttggtattcttctgcaggagtcattttgt-aacatattaaataatgtgtattagacttgagctaatttgcttgctgttgctactactttccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG
                                            |  | || ||  |||||  ||   |  ||    |||||  ||  |   | ||||| ||||         |   ||| |||||| | |||||||   |   |||||| || ||||| |||||||| | ||| || |||||||||||||||||||| ||||||
-----------------------------------------gtaatgctttatgccaacatcatagcgatagttctatagacacga-ttgcatggcttgatgtt----tggtgtgtgattccagAAATTGGTTATAGCTACAGTAAAGAAGGAACTGGGTCTTGCTTTCAGGGGTAATCAAAAGATGGTGGTTGAAGCTTTGGAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|58668733|gb|CK057419.1|CK057419
EST:     GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAG                         AAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGG
genomic: GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGG
EST: gi|86903796|gb|CI302442.1|CI302442
EST:     GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAG                         AAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGG
genomic: GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGG
EST: gi|58684734|gb|CK073421.1|CK073421
EST:     GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAG                         AAACTTGTTATAGTGCCCTCAA-GAAAGACCTGGGGCTTGCTTTTAAGGGG
genomic: GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGG
EST: gi|88300311|gb|CI584730.1|CI584730
EST:     GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAG                         AAACTTGTTATAGTGCCCTCAAAGAAAGACCTGG
genomic: GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGG
EST: gi|4715424|gb|AU056541.1|AU056541
EST:     GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAG                         AAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGG
genomic: GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGG
EST: gi|58662078|gb|CK050758.1|CK050758
EST:     GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAG                         AAACTTGTTATAGTGCCCTCGGGGAAAGACCTGGGGCTTGCTTTTAAGGGG
genomic: GTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 60 (upstream exon), 74 (downstream exon)
Score: 28

GACAAAAGTGGTGTTCCACTTGTTGCTCATGAAAAATTCTCAAAGCCTAGAGAGGTTGAGgtgagttaac ... tccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atgtgtattagacttgagctaatttgcttgctgttgctactactttccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG
                agctaat  putative branch site (score: 28)
                                       ctactttcc  putative PPT
                   taattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagttaactgtcatgtctgattctttggtattcttctgcaggagtcattttgtaacatattaaataatgtgtattagacttgagctaatttgcttgctgttgctactactttccattgcagAAACTTGTTATAGTGCCCTCAAAGAAAGACCTGGGGCTTGCTTTTAAGGGGAACCAAAAGATGGTGGTTGAAGCATTGGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGGAG