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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaatttgactgacctatgaactctgatgtgagcatttgcaaagcctgagcgacttttttcatgcccccatttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATCTCAGAGCTTGGCTATGGAAGAACACCGGGCGCTGATCAATCCTAGCCCC

Basic information

species Oryza sativa
transcript LOC_Os05g46360.1
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os05g46360.1 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G021406_T01 (Zea mays), 3'ss of exon 6
-------gtaatttgactgacctatgaac---tctgatgtgagca------tttgcaaagcctgagcgacttttttcatgcccccatttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATCTCAGAGCTTGGCTATGGAAGAACACCGGGCGCTGATCAATCCTAGCCCC
       |  |  |  ||     ||| ||   |  ||| |    |      |||      | |  || |  | ||      |      ||    ||||||||||| ||| ||||||||||||| ||||| || || |||||||||||||| ||||| ||||| || |||  |   | ||| |      ||  ||| |
gtggcgagctagctagctagtacatgtacatcttcgatatacatacgctttttttgttcacgtacgctagctgttgtggctctttgcctgtgtccagCAATACACGCGGGTGCACCAGGAGGTAGCCCCAGCGACGAGCTGGGAGATCTCGGAGCTCGGCTACGGCAGAGGAGGAGACGCCGGCGCCGCCACGCCGC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|86435016|gb|CI106249.1|CI106249
EST:     AAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAG                         CAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATC
genomic: AAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAGgtaatttgac ... ttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATC
EST: gi|88515323|gb|CI442977.1|CI442977
EST:     AAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAG                         CAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATC
genomic: AAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAGgtaatttgac ... ttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATC
EST: gi|5005166|gb|AU070328.1|AU070328
EST:     AATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGCAG                         CAATACACTC-GTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGAT
genomic: AATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGG-AGgtaatttgac ... ttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGAT
EST: gi|112786818|gb|EE591496.1|EE591496
EST:     AAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAG                         CAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATC
genomic: AAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAGgtaatttgac ... ttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATC
EST: gi|86866134|gb|CI336840.1|CI336840
EST:     AATATTTGTATCTGCACCAACTGCAAGTGAAGTTGCTGACAAAGATGGAG                         CAATACACTCGGTTGCACCAGGAGGGTGGCCCCTGCAACAAGCTGGGAGAT
genomic: AATATTTGTATCTGCACCAACTGCAAGTGAA-TTGCTGACAAAGATGGAGgtaatttgac ... ttggactcagCAATACACTCGGTTGCACCAGGA-GGTGGCCCCTGCAACAAGCTGGGAGAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 65 (upstream exon), 65 (downstream exon)
Score: 8

GTTGTTCGACAAAGGCGAGGCGGAGGGCTTCATCAACTCTGATTGTAGACAAATATTTGTATCTGCACCAACTGCAAGTGAATTGCTGACAAAGATGGAGgtaatttgac ... ttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATCTCAGAGCTTGGCTATGGAAGAACACCGGGCGCTGATCAATCCTAGCCCC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atgtgagcatttgcaaagcctgagcgacttttttcatgcccccatttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATCTCAGAGCTTGGCTATGGAAGAACACCGGGCGCTGATCAATCCTAGCCCC
 ctctgat  putative branch site (score: 8)
                                      cccccattt  putative PPT
                            ttttttcat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaatttgactgacctatgaactctgatgtgagcatttgcaaagcctgagcgacttttttcatgcccccatttggactcagCAATACACTCGGTTGCACCAGGAGGTGGCCCCTGCAACAAGCTGGGAGATCTCAGAGCTTGGCTATGGAAGAACACCGGGCGCTGATCAATCCTAGCCCC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGAT